Gene description for ISCU
Gene name iron-sulfur cluster assembly enzyme
Gene symbol ISCU
Other names/aliases 2310020H20Rik
HML
ISU2
NIFU
NIFUN
hnifU
Species Homo sapiens
 Database cross references - ISCU
ExoCarta ExoCarta_23479
Vesiclepedia VP_23479
Entrez Gene 23479
HGNC 29882
MIM 611911
UniProt Q9H1K1  
 ISCU identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for ISCU
Molecular Function
    iron ion binding GO:0005506 TAS
    protein binding GO:0005515 IPI
    ferrous iron binding GO:0008198 IBA
    zinc ion binding GO:0008270 IDA
    protein homodimerization activity GO:0042803 IDA
    2 iron, 2 sulfur cluster binding GO:0051537 IBA
    molecular adaptor activity GO:0060090 IDA
Biological Process
    intracellular iron ion homeostasis GO:0006879 IBA
    intracellular iron ion homeostasis GO:0006879 IDA
    iron-sulfur cluster assembly GO:0016226 IMP
    iron-sulfur cluster assembly GO:0016226 NAS
    iron-sulfur cluster assembly GO:0016226 TAS
    [2Fe-2S] cluster assembly GO:0044571 IDA
    [4Fe-4S] cluster assembly GO:0044572 IDA
    [4Fe-4S] cluster assembly GO:0044572 IMP
    positive regulation of mitochondrial electron transport, NADH to ubiquinone GO:1902958 IMP
    positive regulation of aconitate hydratase activity GO:1904234 IMP
    negative regulation of iron ion import across plasma membrane GO:1904439 IMP
Subcellular Localization
    nucleus GO:0005634 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 NAS
    mitochondrial matrix GO:0005759 IBA
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    mitochondrial [2Fe-2S] assembly complex GO:0099128 IDA
    iron-sulfur cluster assembly complex GO:1990229 NAS
 Experiment description of studies that identified ISCU in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ISCU
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 BANP  
Two-hybrid Homo sapiens
3 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
4 SFN 2810
Affinity Capture-MS Homo sapiens
5 CS 1431
Proximity Label-MS Homo sapiens
6 NECAB2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 THEM5  
Affinity Capture-MS Homo sapiens
8 DVL2 1856
Affinity Capture-MS Homo sapiens
9 MDH2 4191
Proximity Label-MS Homo sapiens
10 GOLGA6L9  
Two-hybrid Homo sapiens
11 YWHAE 7531
Affinity Capture-MS Homo sapiens
12 CCDC172  
Two-hybrid Homo sapiens
13 LONRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TMEM184A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 KRT34  
Two-hybrid Homo sapiens
16 PDHA1 5160
Proximity Label-MS Homo sapiens
17 NFS1 9054
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 CUL3 8452
Affinity Capture-MS Homo sapiens
19 YWHAZ 7534
Affinity Capture-MS Homo sapiens
20 EGFR 1956
Negative Genetic Homo sapiens
21 IKZF1  
Two-hybrid Homo sapiens
22 GOLGA2 2801
Two-hybrid Homo sapiens
23 AGTRAP 57085
Two-hybrid Homo sapiens
24 HSCB 150274
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 LNX1  
Two-hybrid Homo sapiens
26 SPERT  
Two-hybrid Homo sapiens
27 NUP62 23636
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 HPRT1 3251
Two-hybrid Homo sapiens
29 KRT40  
Two-hybrid Homo sapiens
30 KRAS 3845
Negative Genetic Homo sapiens
31 LYRM4 57128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 FAM9B  
Two-hybrid Homo sapiens
33 IMPA1 3612
Co-fractionation Homo sapiens
34 CCDC136  
Two-hybrid Homo sapiens
35 MID2 11043
Two-hybrid Homo sapiens
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