Gene description for AGTRAP
Gene name angiotensin II receptor-associated protein
Gene symbol AGTRAP
Other names/aliases ATRAP
Species Homo sapiens
 Database cross references - AGTRAP
ExoCarta ExoCarta_57085
Vesiclepedia VP_57085
Entrez Gene 57085
HGNC 13539
MIM 608729
UniProt Q6RW13  
 AGTRAP identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for AGTRAP
Molecular Function
    angiotensin type II receptor activity GO:0004945 IEA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    response to hypoxia GO:0001666 IEA
    regulation of blood pressure GO:0008217 IBA
    angiotensin-activated signaling pathway GO:0038166 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IBA
    cell cortex GO:0005938 IEA
    cytoplasmic vesicle membrane GO:0030659 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified AGTRAP in exosomes
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for AGTRAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF391  
Two-hybrid Homo sapiens
2 TMEM14B  
Two-hybrid Homo sapiens
3 ARHGAP1 392
Two-hybrid Homo sapiens
4 MBLAC2 153364
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ADCK3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 HIST1H4G 8369
Two-hybrid Homo sapiens
7 PTPN9 5780
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 ZFYVE21  
Two-hybrid Homo sapiens
9 HSFX2  
Two-hybrid Homo sapiens
10 CD79A  
Two-hybrid Homo sapiens
11 MRM1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 PBX3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 CELA3A  
Two-hybrid Homo sapiens
14 DDA1  
Two-hybrid Homo sapiens
15 SMG9 56006
Two-hybrid Homo sapiens
16 STX1A 6804
Two-hybrid Homo sapiens
17 TFAM 7019
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 SSX5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 TMPPE  
Two-hybrid Homo sapiens
20 HARS2 23438
Two-hybrid Homo sapiens
21 FAM114A1 92689
Two-hybrid Homo sapiens
22 YARS2  
Two-hybrid Homo sapiens
23 DDX58 23586
Affinity Capture-RNA Homo sapiens
24 AGTR1  
Reconstituted Complex Homo sapiens
25 TCEANC2  
Two-hybrid Homo sapiens
26 CPLX1 10815
Two-hybrid Homo sapiens
27 LYRM5  
Two-hybrid Homo sapiens
28 KIR2DL3  
Two-hybrid Homo sapiens
29 GPX8 493869
Two-hybrid Homo sapiens
30 ACSF2 80221
Two-hybrid Homo sapiens
31 MTERF3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 NFU1 27247
Two-hybrid Homo sapiens
33 KIR3DL3  
Two-hybrid Homo sapiens
34 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 FARS2 10667
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 AP3M1 26985
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 FAM96A  
Two-hybrid Homo sapiens
38 SPG21 51324
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 STAR  
Two-hybrid Homo sapiens
40 SENP2 59343
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
41 ISCU 23479
Two-hybrid Homo sapiens
42 CYB5D2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 TMEM47 83604
Affinity Capture-MS Homo sapiens
44 TCEA2  
Two-hybrid Homo sapiens
45 SCAND1  
Two-hybrid Homo sapiens
46 HSCB 150274
Two-hybrid Homo sapiens
47 MUTYH  
Two-hybrid Homo sapiens
48 AGTRAP 57085
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
49 DIABLO 56616
Two-hybrid Homo sapiens
50 ANKS6 203286
Two-hybrid Homo sapiens
51 ALDH18A1 5832
Two-hybrid Homo sapiens
52 UBE2I 7329
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 RAB30 27314
Two-hybrid Homo sapiens
54 TUBA1B 10376
Two-hybrid Homo sapiens
55 GTF2H1 2965
Two-hybrid Homo sapiens
56 PMAIP1  
Affinity Capture-MS Homo sapiens
57 PDHX 8050
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 HSBP1L1  
Two-hybrid Homo sapiens
59 CD160  
Two-hybrid Homo sapiens
60 C12orf10 60314
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 PRPF18  
Two-hybrid Homo sapiens
62 MRPL11 65003
Two-hybrid Homo sapiens
63 PITPNC1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
64 C17orf62 79415
Two-hybrid Homo sapiens
65 NDUFAF3 25915
Two-hybrid Homo sapiens
66 TXN2 25828
Two-hybrid Homo sapiens
67 SNX1 6642
Two-hybrid Homo sapiens
68 PCTP  
Two-hybrid Homo sapiens
69 FNDC9  
Two-hybrid Homo sapiens
70 TMEM106B 54664
Affinity Capture-MS Homo sapiens
71 MRRF  
Two-hybrid Homo sapiens
72 FFAR2  
Two-hybrid Homo sapiens
73 LRRC59 55379
Two-hybrid Homo sapiens
74 HSFX1  
Two-hybrid Homo sapiens
75 GTF3C1  
Two-hybrid Homo sapiens
76 SSX3  
Two-hybrid Homo sapiens
77 ERGIC3 51614
Two-hybrid Homo sapiens
78 MMD  
Two-hybrid Homo sapiens
79 LRRC4C  
Two-hybrid Homo sapiens
80 HSD17B13  
Two-hybrid Homo sapiens
81 GPR152  
Two-hybrid Homo sapiens
82 FAM209A 200232
Two-hybrid Homo sapiens
83 NDRG4 65009
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
84 IL7R  
Two-hybrid Homo sapiens
85 FAM134C 162427
Two-hybrid Homo sapiens
86 TPD52L3  
Two-hybrid Homo sapiens
87 FAM64A 54478
Two-hybrid Homo sapiens
88 ATP5E 514
Two-hybrid Homo sapiens
89 HIBADH 11112
Two-hybrid Homo sapiens
90 CPLX4  
Two-hybrid Homo sapiens
91 DGCR6  
Two-hybrid Homo sapiens
92 TNFRSF10C  
Affinity Capture-MS Homo sapiens
93 HSPA4 3308
Two-hybrid Homo sapiens
94 PNPO 55163
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
95 RMDN2 151393
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
96 KIAA0368 23392
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
97 RBFA  
Two-hybrid Homo sapiens
98 NKG7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
99 FATE1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 ARAF 369
Two-hybrid Homo sapiens
101 TMEM63A 9725
Affinity Capture-MS Homo sapiens
102 DDX55  
Two-hybrid Homo sapiens
103 TTPA  
Two-hybrid Homo sapiens
104 PPIF 10105
Two-hybrid Homo sapiens
105 MIEF1  
Two-hybrid Homo sapiens
106 FAM160A2 84067
Two-hybrid Homo sapiens
107 GAD2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
108 AGTR2  
Reconstituted Complex Homo sapiens
109 HMGB1 3146
Two-hybrid Homo sapiens
110 SYT16  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
111 CHRNB1  
Affinity Capture-MS Homo sapiens
112 LAMP5  
Affinity Capture-MS Homo sapiens
113 GAD1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
114 PDHA1 5160
Affinity Capture-MS Homo sapiens
115 GABARAPL2 11345
Two-hybrid Homo sapiens
116 LMO2  
Two-hybrid Homo sapiens
117 TMEM139  
Two-hybrid Homo sapiens
118 CAPNS1 826
Two-hybrid Homo sapiens
119 SUCLA2 8803
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
120 PAFAH2 5051
Affinity Capture-MS Homo sapiens
121 BPIFA2 140683
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
122 ARFIP2 23647
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
123 APOA4 337
Two-hybrid Homo sapiens
124 TP53 7157
Affinity Capture-MS Homo sapiens
125 STAG3L1  
Two-hybrid Homo sapiens
126 FASTKD3  
Two-hybrid Homo sapiens
127 NFYB  
Two-hybrid Homo sapiens
128 TBRG4 9238
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
129 THAP4 51078
Two-hybrid Homo sapiens
130 MIEF2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
131 KCNK5  
Two-hybrid Homo sapiens
132 DNAJC1 64215
Two-hybrid Homo sapiens
133 GDNF  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
134 GNB2L1 10399
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
135 TMEM31  
Two-hybrid Homo sapiens
136 STARD4  
Two-hybrid Homo sapiens
137 MCEE 84693
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
138 PCYT1A 5130
Two-hybrid Homo sapiens
139 CCDC70  
Two-hybrid Homo sapiens
140 LNX1  
Two-hybrid Homo sapiens
141 MGST3 4259
Two-hybrid Homo sapiens
142 CRCT1  
Two-hybrid Homo sapiens
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