Gene description for NFU1
Gene name NFU1 iron-sulfur cluster scaffold
Gene symbol NFU1
Other names/aliases CGI-33
HIRIP
HIRIP5
MMDS1
NIFUC
Nfu
NifU
Species Homo sapiens
 Database cross references - NFU1
ExoCarta ExoCarta_27247
Vesiclepedia VP_27247
Entrez Gene 27247
HGNC 16287
MIM 608100
UniProt Q9UMS0  
 NFU1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NFU1
Molecular Function
    iron ion binding GO:0005506 IDA
    protein binding GO:0005515 IPI
    2 iron, 2 sulfur cluster binding GO:0051537 IDA
    4 iron, 4 sulfur cluster binding GO:0051539 IBA
    4 iron, 4 sulfur cluster binding GO:0051539 IDA
Biological Process
    iron-sulfur cluster assembly GO:0016226 IDA
    protein maturation by [2Fe-2S] cluster transfer GO:0106034 IDA
    protein maturation by [4Fe-4S] cluster transfer GO:0106035 IBA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified NFU1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NFU1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MORN3  
Two-hybrid Homo sapiens
2 PDHA1 5160
Proximity Label-MS Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
4 CS 1431
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
5 SOD2 6648
Co-fractionation Homo sapiens
6 COIL  
Two-hybrid Homo sapiens
7 HTRA2 27429
Co-fractionation Homo sapiens
8 CUTA 51596
Co-fractionation Homo sapiens
9 PITRM1 10531
Co-fractionation Homo sapiens
10 DESI2  
Two-hybrid Homo sapiens
11 YWHAB 7529
Co-fractionation Homo sapiens
12 SH3BP4 23677
Two-hybrid Homo sapiens
13 ABHD10 55347
Co-fractionation Homo sapiens
14 PTEN 5728
Negative Genetic Homo sapiens
15 DLD 1738
Co-fractionation Homo sapiens
16 MTG2  
Proximity Label-MS Homo sapiens
17 AGTRAP 57085
Two-hybrid Homo sapiens
18 MDK 4192
Two-hybrid Homo sapiens
19 SUCLG1 8802
Co-fractionation Homo sapiens
20 HSPB1 3315
Two-hybrid Homo sapiens
21 ECI1 1632
Co-fractionation Homo sapiens
22 HMGCS1 3157
Co-fractionation Homo sapiens
23 SUCLA2 8803
Co-fractionation Homo sapiens
24 APOC4  
Two-hybrid Homo sapiens
25 C21orf33  
Proximity Label-MS Homo sapiens
26 OAT 4942
Co-fractionation Homo sapiens
27 DUSP13  
Affinity Capture-MS Homo sapiens
28 APOC1  
Two-hybrid Homo sapiens
29 ZSCAN5A  
Two-hybrid Homo sapiens
30 EPM2A  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
31 TRIM23 373
Two-hybrid Homo sapiens
32 CMTM5  
Two-hybrid Homo sapiens
33 ACO2 50
Affinity Capture-MS Homo sapiens
34 REXO2 25996
Co-fractionation Homo sapiens
35 AUH 549
Proximity Label-MS Homo sapiens
36 HIRA  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
37 MDH1 4190
Co-fractionation Homo sapiens
38 CALCOCO2  
Two-hybrid Homo sapiens
39 CIDEB  
Two-hybrid Homo sapiens
40 TFIP11  
Two-hybrid Homo sapiens
41 Prkaa1  
Affinity Capture-MS Mus musculus
42 SDCBP2 27111
Two-hybrid Homo sapiens
43 MCUR1 63933
Proximity Label-MS Homo sapiens
44 APOC2 344
Two-hybrid Homo sapiens
45 ZNF688  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NFU1 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Post-translational protein modification TAS Reactome
Protein lipoylation TAS Reactome





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