Gene description for HSPB1
Gene name heat shock 27kDa protein 1
Gene symbol HSPB1
Other names/aliases CMT2F
HEL-S-102
HMN2B
HS.76067
HSP27
HSP28
Hsp25
SRP27
Species Homo sapiens
 Database cross references - HSPB1
ExoCarta ExoCarta_3315
Vesiclepedia VP_3315
Entrez Gene 3315
HGNC 5246
MIM 602195
UniProt P04792  
 HSPB1 identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 19415654    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Keratinocytes 19530224    
Keratinocytes 19530224    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
Thymus 23844026    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for HSPB1
Molecular Function
    RNA binding GO:0003723 HDA
    protein kinase C binding GO:0005080 ISS
    protein binding GO:0005515 IPI
    protein kinase C inhibitor activity GO:0008426 ISS
    protein kinase binding GO:0019901 IPI
    identical protein binding GO:0042802 IMP
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    ubiquitin binding GO:0043130 ISS
    protein folding chaperone GO:0044183 IMP
    unfolded protein binding GO:0051082 IBA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
Biological Process
    regulation of protein phosphorylation GO:0001932 IMP
    regulation of translational initiation GO:0006446 TAS
    negative regulation of protein kinase activity GO:0006469 ISS
    response to unfolded protein GO:0006986 NAS
    response to heat GO:0009408 IBA
    response to virus GO:0009615 IEP
    regulation of autophagy GO:0010506 NAS
    positive regulation of interleukin-1 beta production GO:0032731 ISS
    positive regulation of tumor necrosis factor production GO:0032760 ISS
    intracellular signal transduction GO:0035556 IMP
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IMP
    protein refolding GO:0042026 IBA
    negative regulation of apoptotic process GO:0043066 IBA
    negative regulation of apoptotic process GO:0043066 TAS
    regulation of canonical NF-kappaB signal transduction GO:0043122 ISS
    positive regulation of blood vessel endothelial cell migration GO:0043536 IMP
    positive regulation of angiogenesis GO:0045766 IMP
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IMP
    chaperone-mediated protein folding GO:0061077 IMP
    platelet aggregation GO:0070527 HMP
    anterograde axonal protein transport GO:0099641 IMP
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 ISS
    positive regulation of endothelial cell chemotaxis GO:2001028 IMP
Subcellular Localization
    proteasome complex GO:0000502 ISS
    cornified envelope GO:0001533 IEA
    extracellular space GO:0005615 HDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 TAS
    spindle GO:0005819 IEA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 TAS
    focal adhesion GO:0005925 HDA
    Z disc GO:0030018 IEA
    extracellular exosome GO:0070062 HDA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified HSPB1 in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 65
MISEV standards
✘ Biophysical techniques
✔
HSP60|HSP27|GAPDH|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 19415654    
Organism Homo sapiens
Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7.
Authors "Staubach S, Razawi H, Hanisch FG."
Journal name PROTEOMICS
Publication year 2009
Sample Breast cancer cells
Sample name MCF-7
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
7
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
8
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
9
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
15
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
19
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
20
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
29
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
30
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
31
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
32
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
33
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
34
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
35
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
37
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
38
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
39
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
40
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
41
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 219
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name C666-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
43
Experiment ID 220
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP69
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
44
Experiment ID 221
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP460
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
45
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
46
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
47
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
48
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
49
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
50
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
51
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
52
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
53
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
54
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
55
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
56
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
57
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
58
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
59
Experiment ID 136
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name PC3 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
60
Experiment ID 143
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
61
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
62
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
63
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
64
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
65
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
66
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
67
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
68
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
69
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
70
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
71
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HSPB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ISG15 9636
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 OXCT1 5019
Co-fractionation Homo sapiens
3 UBL4A 8266
Affinity Capture-MS Homo sapiens
4 CRYGC  
Reconstituted Complex Bos taurus
5 PKM 5315
Co-fractionation Homo sapiens
6 CRK 1398
Co-fractionation Homo sapiens
7 EBNA-LP  
Affinity Capture-MS
8 MAP4 4134
Co-fractionation Homo sapiens
9 KIF20A 10112
Affinity Capture-MS Homo sapiens
10 UBC 7316
Affinity Capture-MS Homo sapiens
11 PRPF40A 55660
Two-hybrid Homo sapiens
12 HDAC4  
Affinity Capture-MS Homo sapiens
13 IGBP1 3476
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 CRNKL1 51340
Two-hybrid Homo sapiens
15 STRN 6801
Affinity Capture-MS Homo sapiens
16 KIF23 9493
Affinity Capture-MS Homo sapiens
17 OSBPL9 114883
Two-hybrid Homo sapiens
18 CTGLF10P  
Two-hybrid Homo sapiens
19 ZFAT  
Two-hybrid Homo sapiens
20 C15orf57  
Two-hybrid Homo sapiens
21 PPM1A 5494
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 AMD1  
Two-hybrid Homo sapiens
23 UCHL5 51377
Reconstituted Complex Homo sapiens
24 BTRC 8945
Affinity Capture-MS Homo sapiens
25 GNPAT 8443
Two-hybrid Homo sapiens
26 EEF1A1 1915
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
27 RAD23A 5886
Two-hybrid Homo sapiens
28 KIF14 9928
Affinity Capture-MS Homo sapiens
29 GOLGA8A 23015
Two-hybrid Homo sapiens
30 ZYX 7791
Co-fractionation Homo sapiens
31 HN1L 90861
Co-fractionation Homo sapiens
32 MTCH2 23788
Affinity Capture-MS Homo sapiens
33 USP14 9097
Co-fractionation Homo sapiens
34 PELO 53918
Two-hybrid Homo sapiens
35 LUZP1 7798
Two-hybrid Homo sapiens
36 TARBP2  
Two-hybrid Homo sapiens
37 SUPT5H 6829
Two-hybrid Homo sapiens
38 SRSF3 6428
Co-fractionation Homo sapiens
39 BVES  
Two-hybrid Homo sapiens
40 PABPC1 26986
Co-fractionation Homo sapiens
41 SERTAD1  
Two-hybrid Homo sapiens
42 YAP1 10413
Affinity Capture-MS Homo sapiens
43 TYK2 7297
Affinity Capture-MS Homo sapiens
44 TRIM21 6737
Affinity Capture-MS Homo sapiens
45 SLX4  
Affinity Capture-MS Homo sapiens
46 NOS2  
Affinity Capture-MS Homo sapiens
47 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
48 CRYGC  
Two-hybrid Homo sapiens
49 HECTD1 25831
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
50 DDX39B 7919
Affinity Capture-MS Homo sapiens
51 HSPB8 26353
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
52 TRIM63  
Two-hybrid Homo sapiens
53 CCNT1  
Affinity Capture-MS Homo sapiens
54 FAM71D  
Two-hybrid Homo sapiens
55 GTSF1  
Two-hybrid Homo sapiens
56 YWHAE 7531
Two-hybrid Homo sapiens
57 ANLN 54443
Affinity Capture-MS Homo sapiens
58 EPB41L1 2036
Two-hybrid Homo sapiens
59 ZBTB17  
Two-hybrid Homo sapiens
60 RBM39 9584
Two-hybrid Homo sapiens
61 DNAJC21  
Two-hybrid Homo sapiens
62 MTIF2 4528
Two-hybrid Homo sapiens
63 SDHA 6389
Co-fractionation Homo sapiens
64 CAST 831
Co-fractionation Homo sapiens
65 GRIPAP1 56850
Two-hybrid Homo sapiens
66 RSPH3  
Two-hybrid Homo sapiens
67 CRYAB 1410
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
68 IQCB1  
Affinity Capture-MS Homo sapiens
69 KCMF1 56888
Two-hybrid Homo sapiens
70 PRKCZ 5590
Affinity Capture-MS Homo sapiens
71 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 DES 1674
Two-hybrid Homo sapiens
73 EEF1B2 1933
Co-fractionation Homo sapiens
74 SBF1 6305
Two-hybrid Homo sapiens
75 DUSP16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 FN1 2335
Affinity Capture-MS Homo sapiens
77 PITPNA 5306
Co-fractionation Homo sapiens
78 GLO1 2739
Co-fractionation Homo sapiens
79 ARMC6 93436
Affinity Capture-MS Homo sapiens
80 UBA2 10054
Co-fractionation Homo sapiens
81 MFAP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
82 RPA3 6119
Affinity Capture-MS Homo sapiens
83 LSM3 27258
Two-hybrid Homo sapiens
84 C11orf98  
Two-hybrid Homo sapiens
85 DLST 1743
Affinity Capture-MS Homo sapiens
86 HSPA1A 3303
Co-fractionation Homo sapiens
87 HSPA4 3308
Two-hybrid Homo sapiens
88 STMN1 3925
Co-fractionation Homo sapiens
89 CARHSP1 23589
Co-fractionation Homo sapiens
90 ILK 3611
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 AKT1 207
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
92 STUB1 10273
Two-hybrid Homo sapiens
93 PSMB2 5690
Two-hybrid Homo sapiens
94 ERICH6  
Two-hybrid Homo sapiens
95 AATK  
Affinity Capture-MS Homo sapiens
96 PSAT1 29968
Co-fractionation Homo sapiens
97 WDR66  
Two-hybrid Homo sapiens
98 ARHGDIA 396
Co-fractionation Homo sapiens
99 RBM25 58517
Two-hybrid Homo sapiens
100 WWOX 51741
Affinity Capture-MS Homo sapiens
101 DFFA 1676
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
102 USP1 7398
Affinity Capture-MS Homo sapiens
103 CETN3  
Two-hybrid Homo sapiens
104 PNISR  
Two-hybrid Homo sapiens
105 TKT 7086
Co-fractionation Homo sapiens
106 HSP90AA1 3320
Two-hybrid Homo sapiens
107 TARS 6897
Two-hybrid Homo sapiens
108 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
109 GAPDH 2597
Co-fractionation Homo sapiens
110 EIF4B 1975
Co-fractionation Homo sapiens
111 LUCAT1  
Affinity Capture-RNA Homo sapiens
112 ITPA 3704
Co-fractionation Homo sapiens
113 IGSF21  
Two-hybrid Homo sapiens
114 CEP78 84131
Two-hybrid Homo sapiens
115 EPHA2 1969
Proximity Label-MS Homo sapiens
116 PTGES3 10728
Two-hybrid Homo sapiens
117 SNW1 22938
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
118 SMURF2 64750
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
119 MRPL28 10573
Two-hybrid Homo sapiens
120 NVL  
Two-hybrid Homo sapiens
121 EIF4G3 8672
Two-hybrid Homo sapiens
122 PSMA6 5687
Two-hybrid Homo sapiens
123 PDHA1 5160
Affinity Capture-MS Homo sapiens
124 ZBTB37  
Two-hybrid Homo sapiens
125 IARS2 55699
Two-hybrid Homo sapiens
126 SPRTN  
Affinity Capture-MS Homo sapiens
127 ACAP2 23527
Two-hybrid Homo sapiens
128 PTPN22  
Affinity Capture-MS Homo sapiens
129 ZNRF3  
Two-hybrid Homo sapiens
130 TARDBP 23435
Co-fractionation Homo sapiens
131 RPA2 6118
Affinity Capture-MS Homo sapiens
132 ALB 213
Co-fractionation Homo sapiens
133 HSP90AB1 3326
Two-hybrid Homo sapiens
134 SNRPF 6636
Two-hybrid Homo sapiens
135 UAP1 6675
Co-fractionation Homo sapiens
136 SAFB 6294
Co-fractionation Homo sapiens
137 RBM48  
Two-hybrid Homo sapiens
138 S100A4 6275
Two-hybrid Homo sapiens
139 B3GNT2 10678
Affinity Capture-MS Homo sapiens
140 CDC123 8872
Two-hybrid Homo sapiens
141 SPIN1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
142 CSRP1 1465
Co-fractionation Homo sapiens
143 WDR83  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
144 PRC1 9055
Affinity Capture-MS Homo sapiens
145 MRPS23 51649
Two-hybrid Homo sapiens
146 SRRM2 23524
Affinity Capture-MS Homo sapiens
147 CCDC53 51019
Two-hybrid Homo sapiens
148 ARMT1 79624
Two-hybrid Homo sapiens
149 OBSL1 23363
Two-hybrid Homo sapiens
150 STIP1 10963
Co-fractionation Homo sapiens
151 ADRBK2  
Two-hybrid Homo sapiens
152 YWHAH 7533
Two-hybrid Homo sapiens
153 VCAM1 7412
Affinity Capture-MS Homo sapiens
154 PALLD 23022
Co-fractionation Homo sapiens
155 AMBRA1  
Affinity Capture-MS Homo sapiens
156 CDC5L 988
Affinity Capture-MS Homo sapiens
157 NPAS1  
Affinity Capture-MS Homo sapiens
158 TBC1D1 23216
Two-hybrid Homo sapiens
159 YWHAB 7529
Two-hybrid Homo sapiens
160 KANSL1  
Two-hybrid Homo sapiens
161 MYL9 10398
Two-hybrid Homo sapiens
162 SPACA7  
Two-hybrid Homo sapiens
163 SEPHS1 22929
Co-fractionation Homo sapiens
164 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
165 RBX1 9978
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
166 KLC1 3831
Two-hybrid Homo sapiens
167 PALM3  
Two-hybrid Homo sapiens
168 OPTN 10133
Two-hybrid Homo sapiens
169 PSMD14 10213
Affinity Capture-MS Homo sapiens
170 LCE3A  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
171 AHNAK 79026
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
172 TRIM24  
Two-hybrid Homo sapiens
173 SNRNP200 23020
Two-hybrid Homo sapiens
174 TUBA1C 84790
Affinity Capture-MS Homo sapiens
175 PPA1 5464
Two-hybrid Homo sapiens
176 HAUS8  
Two-hybrid Homo sapiens
177 TOX4  
Two-hybrid Homo sapiens
178 PPP2R3C  
Two-hybrid Homo sapiens
179 ASB10  
Affinity Capture-MS Homo sapiens
180 GMPS 8833
Co-fractionation Homo sapiens
181 ARHGAP28 79822
Two-hybrid Homo sapiens
182 KCTD3  
Two-hybrid Homo sapiens
183 CYLD  
Affinity Capture-MS Homo sapiens
184 HNRNPD 3184
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
185 UFD1L 7353
Two-hybrid Homo sapiens
186 TSNAXIP1 55815
Two-hybrid Homo sapiens
187 PSMA3 5684
Two-hybrid Homo sapiens
188 AGAP4  
Two-hybrid Homo sapiens
189 CCNC  
Two-hybrid Homo sapiens
190 XPO1 7514
Two-hybrid Homo sapiens
191 GLMN 11146
Two-hybrid Homo sapiens
192 RC3H2  
Affinity Capture-MS Homo sapiens
193 SEC13 6396
Two-hybrid Homo sapiens
194 ADCY9 115
Two-hybrid Homo sapiens
195 JADE1  
Affinity Capture-MS Homo sapiens
196 EGFR 1956
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
197 FTH1 2495
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
198 LGALS7 3963
Affinity Capture-MS Homo sapiens
199 RIPK4  
Affinity Capture-MS Homo sapiens
200 SPTBN1 6711
Two-hybrid Homo sapiens
201 G6PD 2539
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
202 TOMM70A 9868
Two-hybrid Homo sapiens
203 EFTUD2 9343
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
204 DMAP1 55929
Two-hybrid Homo sapiens
205 HSPB6 126393
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
206 HNRNPH1 3187
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
207 NRD1 4898
Co-fractionation Homo sapiens
208 SLC7A9  
Two-hybrid Homo sapiens
209 lwr  
Two-hybrid Drosophila melanogaster
210 F13A1 2162
Co-localization Homo sapiens
211 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
212 KRT18 3875
Two-hybrid Homo sapiens
213 TES 26136
Co-fractionation Homo sapiens
214 TAF6 6878
Co-fractionation Homo sapiens
215 HNRNPH2 3188
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
216 ILF3 3609
Co-fractionation Homo sapiens
217 ARRDC5  
Two-hybrid Homo sapiens
218 TP53 7157
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
219 FOLR1 2348
Affinity Capture-MS Homo sapiens
220 CCHCR1  
Two-hybrid Homo sapiens
221 PEX19 5824
Co-fractionation Homo sapiens
222 EIF1AX 1964
Two-hybrid Homo sapiens
223 FXR1 8087
Two-hybrid Homo sapiens
224 HDAC6 10013
Affinity Capture-Western Homo sapiens
225 PTBP1 5725
Co-fractionation Homo sapiens
226 SQSTM1 8878
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
227 FKBP4 2288
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
228 MAP2K1 5604
Affinity Capture-MS Homo sapiens
229 AARSD1 80755
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
230 ESR1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
231 EPB41L3 23136
Two-hybrid Homo sapiens
232 BCL2L1 598
Affinity Capture-MS Homo sapiens
233 EEF1D 1936
Co-fractionation Homo sapiens
234 GAPVD1 26130
Two-hybrid Homo sapiens
235 PUM1 9698
Co-fractionation Homo sapiens
236 KRAS 3845
Affinity Capture-MS Homo sapiens
237 CYCS 54205
Affinity Capture-Western Homo sapiens
238 PSMD1 5707
Two-hybrid Homo sapiens
239 IKBKB 3551
Affinity Capture-Western Homo sapiens
240 CPSF3 51692
Two-hybrid Homo sapiens
241 MYL12B 103910
Two-hybrid Homo sapiens
242 GATA1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
243 RPSA 3921
Two-hybrid Homo sapiens
244 CBX1 10951
Two-hybrid Homo sapiens
245 PRKCD 5580
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
246 SPATA7 55812
Two-hybrid Homo sapiens
247 NAPA 8775
Two-hybrid Homo sapiens
248 PHYHIPL  
Two-hybrid Homo sapiens
249 MME 4311
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
250 NBAS 51594
Two-hybrid Homo sapiens
251 ANK1 286
Two-hybrid Homo sapiens
252 BUB1B  
Affinity Capture-MS Homo sapiens
253 ADD3 120
Two-hybrid Homo sapiens
254 UQCRB 7381
Two-hybrid Homo sapiens
255 NCKIPSD 51517
Two-hybrid Homo sapiens
256 TRIM54  
Two-hybrid Homo sapiens
257 ERCC5  
Two-hybrid Homo sapiens
258 UGDH 7358
Co-fractionation Homo sapiens
259 MCM2 4171
Affinity Capture-MS Homo sapiens
260 CASP3 836
Affinity Capture-Western Homo sapiens
261 KPNA3 3839
Two-hybrid Homo sapiens
262 LIG1 3978
Two-hybrid Homo sapiens
263 LASP1 3927
Co-fractionation Homo sapiens
264 MCM6 4175
Two-hybrid Homo sapiens
265 ITGA4 3676
Affinity Capture-MS Homo sapiens
266 CALR 811
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
267 CCNDBP1 23582
Two-hybrid Homo sapiens
268 SMARCA2 6595
Two-hybrid Homo sapiens
269 UQCC2  
Two-hybrid Homo sapiens
270 PABPC4 8761
Co-fractionation Homo sapiens
271 PDLIM1 9124
Co-fractionation Homo sapiens
272 EHD1 10938
Two-hybrid Homo sapiens
273 RBPJ 3516
Two-hybrid Homo sapiens
274 HSPA8 3312
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
275 FOXP1 27086
Protein-RNA Homo sapiens
276 GUCA1A  
Two-hybrid Homo sapiens
277 CENPK  
Two-hybrid Homo sapiens
278 LONRF1  
Two-hybrid Homo sapiens
279 IRS4 8471
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
280 ATP5A1 498
Co-fractionation Homo sapiens
281 IRAK1  
Affinity Capture-MS Homo sapiens
282 RALA 5898
Two-hybrid Homo sapiens
283 TBC1D3B  
Two-hybrid Homo sapiens
284 ALPI  
Two-hybrid Homo sapiens
285 MAPKAPK3 7867
Co-localization Homo sapiens
286 RASSF9  
Two-hybrid Homo sapiens
287 ANKRD35  
Affinity Capture-MS Homo sapiens
288 BTF3 689
Affinity Capture-MS Homo sapiens
289 MSN 4478
Co-fractionation Homo sapiens
290 NTRK1 4914
Affinity Capture-MS Homo sapiens
291 ARNT 405
Affinity Capture-MS Homo sapiens
292 ZNF131  
Two-hybrid Homo sapiens
293 FLNB 2317
Two-hybrid Homo sapiens
294 CHMP4B 128866
Affinity Capture-MS Homo sapiens
295 PHF12  
Affinity Capture-MS Homo sapiens
296 MED31  
Two-hybrid Homo sapiens
297 SAT1  
Two-hybrid Homo sapiens
298 MYL12A 10627
Two-hybrid Homo sapiens
299 TMCO3 55002
Two-hybrid Homo sapiens
300 RYBP  
Two-hybrid Homo sapiens
301 SF3A3 10946
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
302 CAMK1 8536
Two-hybrid Homo sapiens
303 EIF4A2 1974
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
304 ACTN4 81
Two-hybrid Homo sapiens
305 HNRNPDL 9987
Co-fractionation Homo sapiens
306 ACAT1 38
Co-fractionation Homo sapiens
307 OLA1 29789
Co-fractionation Homo sapiens
308 MRPL40 64976
Two-hybrid Homo sapiens
309 EFNA4  
Affinity Capture-MS Homo sapiens
310 GLOD4 51031
Co-fractionation Homo sapiens
311 SLC25A12 8604
Proximity Label-MS Homo sapiens
312 CDC73  
Affinity Capture-MS Homo sapiens
313 PNPT1 87178
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
314 SMARCD1 6602
Two-hybrid Homo sapiens
315 ECT2 1894
Affinity Capture-MS Homo sapiens
316 HN1 51155
Co-fractionation Homo sapiens
317 CRYAA 1409
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
318 PTPRA 5786
Two-hybrid Homo sapiens
319 HYPK 25764
Affinity Capture-MS Homo sapiens
320 USP38 84640
Affinity Capture-MS Homo sapiens
321 PRKD1 5587
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
322 ATXN10 25814
Two-hybrid Homo sapiens
323 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
324 TGFB1I1 7041
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
325 METTL21B  
Affinity Capture-MS Homo sapiens
326 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
327 MAP3K7  
Affinity Capture-Western Homo sapiens
328 INPP5K 51763
Two-hybrid Homo sapiens
329 PRPF19 27339
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
330 MSL1 339287
Two-hybrid Homo sapiens
331 MAPKAPK5 8550
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
332 ACTN2 88
Two-hybrid Homo sapiens
333 HDLBP 3069
Two-hybrid Homo sapiens
334 Kctd5  
Affinity Capture-MS Mus musculus
335 NSMCE4A  
Two-hybrid Homo sapiens
336 KIAA0368 23392
Two-hybrid Homo sapiens
337 SIK2  
Affinity Capture-MS Homo sapiens
338 ASXL1  
Affinity Capture-MS Homo sapiens
339 HSPA9 3313
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
340 ARAF 369
Affinity Capture-MS Homo sapiens
341 CHUK 1147
Affinity Capture-Western Homo sapiens
342 ALG9 79796
Affinity Capture-MS Homo sapiens
343 XRCC5 7520
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
344 ATIC 471
Co-fractionation Homo sapiens
345 TPM3 7170
Co-fractionation Homo sapiens
346 ARHGEF7 8874
Two-hybrid Homo sapiens
347 EIF2S1 1965
Two-hybrid Homo sapiens
348 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
349 RARA 5914
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
350 SMN1 6606
Affinity Capture-Western Homo sapiens
351 JUP 3728
Affinity Capture-MS Homo sapiens
352 PRRC2C 23215
Co-fractionation Homo sapiens
353 RPA1 6117
Affinity Capture-MS Homo sapiens
354 KIAA1429 25962
Affinity Capture-MS Homo sapiens
355 G3BP1 10146
Affinity Capture-MS Homo sapiens
356 HSPB1 3315
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
357 GRPEL1 80273
Co-fractionation Homo sapiens
358 CREBBP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
359 AGAP5  
Two-hybrid Homo sapiens
360 TTC39C 125488
Two-hybrid Homo sapiens
361 EEF2 1938
Co-fractionation Homo sapiens
362 NEK10 152110
Two-hybrid Homo sapiens
363 LRRK2 120892
Affinity Capture-Western Homo sapiens
364 HNRNPH3 3189
Co-fractionation Homo sapiens
365 PRDX6 9588
Co-fractionation Homo sapiens
366 HNRNPF 3185
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
367 ENPP2  
Two-hybrid Homo sapiens
368 SERBP1 26135
Affinity Capture-MS Homo sapiens
369 AR 367
Affinity Capture-MS Homo sapiens
370 RC3H1 149041
Affinity Capture-MS Homo sapiens
371 ATXN3 4287
Affinity Capture-MS Homo sapiens
372 VIM 7431
Two-hybrid Homo sapiens
373 CCDC70  
Two-hybrid Homo sapiens
374 LBP 3929
Two-hybrid Homo sapiens
375 EIF5 1983
Two-hybrid Homo sapiens
376 UBE2H 7328
Affinity Capture-MS Homo sapiens
377 AP2B1 163
Two-hybrid Homo sapiens
378 STAT2 6773
Affinity Capture-Western Homo sapiens
379 WWP1 11059
Two-hybrid Homo sapiens
380 SYCE1  
Two-hybrid Homo sapiens
381 IPO7 10527
Co-fractionation Homo sapiens
382 PAICS 10606
Co-fractionation Homo sapiens
383 MPO 4353
Co-fractionation Homo sapiens
384 TRIM55  
Two-hybrid Homo sapiens
385 ZBTB1  
Affinity Capture-MS Homo sapiens
386 AGAP3 116988
Two-hybrid Homo sapiens
387 HSPA5 3309
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
388 ASB17  
Affinity Capture-MS Homo sapiens
389 C2orf73  
Two-hybrid Homo sapiens
390 TGFB1 7040
Affinity Capture-MS Homo sapiens
391 PITPNB 23760
Co-fractionation Homo sapiens
392 COPS5 10987
Affinity Capture-MS Homo sapiens
393 ZNF197  
Two-hybrid Homo sapiens
394 AHCY 191
Co-fractionation Homo sapiens
395 ASB4  
Affinity Capture-MS Homo sapiens
396 ANAPC7 51434
Two-hybrid Homo sapiens
397 CDK1 983
Co-fractionation Homo sapiens
398 CEP57  
Affinity Capture-MS Homo sapiens
399 MYCN  
Affinity Capture-MS Homo sapiens
400 SARS 6301
Co-fractionation Homo sapiens
401 HSPD1 3329
Co-fractionation Homo sapiens
402 TPT1 7178
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
403 NFU1 27247
Two-hybrid Homo sapiens
404 GARS 2617
Two-hybrid Homo sapiens
405 POLD1 5424
Affinity Capture-MS Homo sapiens
406 DUT 1854
Co-fractionation Homo sapiens
407 CLCN3 1182
Two-hybrid Homo sapiens
408 PSMC1 5700
Two-hybrid Homo sapiens
409 RAB41  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
410 LRIF1  
Two-hybrid Homo sapiens
411 ATF2  
Affinity Capture-MS Homo sapiens
412 UBE2I 7329
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
413 SMARCA4 6597
Two-hybrid Homo sapiens
414 PFDN2 5202
Co-fractionation Homo sapiens
415 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
416 AGAP7P  
Two-hybrid Homo sapiens
417 ERP29 10961
Co-fractionation Homo sapiens
418 C12orf10 60314
Two-hybrid Homo sapiens
419 PCSK9 255738
Affinity Capture-MS Homo sapiens
420 EIF4G2 1982
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
421 ELANE 1991
Co-fractionation Homo sapiens
422 BRAF  
Affinity Capture-MS Homo sapiens
423 DAXX  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
424 CCNO  
Two-hybrid Homo sapiens
425 TCEAL4  
Co-fractionation Homo sapiens
426 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
427 KRT72 140807
Two-hybrid Homo sapiens
428 CCDC64  
Two-hybrid Homo sapiens
429 ACTN1 87
Two-hybrid Homo sapiens
430 UBE2N 7334
Co-fractionation Homo sapiens
431 ANKRD7  
Two-hybrid Homo sapiens
432 FANCD2  
Affinity Capture-MS Homo sapiens
433 OTUD1 220213
Affinity Capture-MS Homo sapiens
434 YWHAZ 7534
Two-hybrid Homo sapiens
435 CRYBB2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
436 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
437 Bmpr1a  
Affinity Capture-MS Mus musculus
438 ASB6 140459
Affinity Capture-MS Homo sapiens
439 ISG20L2 81875
Two-hybrid Homo sapiens
440 UNG  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
441 TTC3 7267
Two-hybrid Homo sapiens
442 PDIA3 2923
Co-fractionation Homo sapiens
443 THOC5 8563
Two-hybrid Homo sapiens
444 MRGBP  
Two-hybrid Homo sapiens
445 NAP1L1 4673
Two-hybrid Homo sapiens
446 GDI2 2665
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
447 AURKB 9212
Affinity Capture-MS Homo sapiens
448 GSTO1 9446
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
449 MAPK6  
Affinity Capture-MS Homo sapiens
450 KNSTRN  
Two-hybrid Homo sapiens
451 TXNL1 9352
Two-hybrid Homo sapiens
452 RPAP3 79657
Two-hybrid Homo sapiens
453 ANXA1 301
Two-hybrid Homo sapiens
454 ADSS 159
Affinity Capture-MS Homo sapiens
455 SF3B3 23450
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
456 RNF114 55905
Co-fractionation Homo sapiens
457 RARS 5917
Co-fractionation Homo sapiens
458 C9orf72  
Affinity Capture-MS Homo sapiens
459 DHX15 1665
Co-fractionation Homo sapiens
460 MAPKAPK2 9261
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
461 NEXN 91624
Two-hybrid Homo sapiens
462 MYLK 4638
Two-hybrid Homo sapiens
463 HSPB2  
Affinity Capture-MS Homo sapiens
464 HAUS1  
Affinity Capture-MS Homo sapiens
465 MPHOSPH6 10200
Two-hybrid Homo sapiens
466 TNF  
Affinity Capture-RNA Homo sapiens
467 HNRNPA2B1 3181
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
468 HMGN5 79366
Co-fractionation Homo sapiens
469 TUBA4A 7277
Co-fractionation Homo sapiens
470 DMWD  
Affinity Capture-MS Homo sapiens
471 SRP72 6731
Two-hybrid Homo sapiens
472 PTPN3  
Two-hybrid Homo sapiens
473 EPB41L2 2037
Two-hybrid Homo sapiens
474 ADPRHL2 54936
Two-hybrid Homo sapiens
475 TCPT  
Affinity Capture-Western Homo sapiens
476 NAMPT 10135
Co-fractionation Homo sapiens
477 PGM2 55276
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
478 PDLIM5 10611
Co-fractionation Homo sapiens
479 MAGED1 9500
Affinity Capture-MS Homo sapiens
480 EZH2  
Affinity Capture-MS Homo sapiens
481 SNCA 6622
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
482 PHLDA1 22822
Affinity Capture-Western Homo sapiens
483 HSF1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
484 ACBD6 84320
Two-hybrid Homo sapiens
485 CDK5RAP3 80279
Two-hybrid Homo sapiens
486 TJP2 9414
Two-hybrid Homo sapiens
487 SRMS  
Affinity Capture-MS Homo sapiens
488 HNRNPU 3192
Two-hybrid Homo sapiens
489 EIF3M 10480
Two-hybrid Homo sapiens
490 PAF1 54623
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
491 CFTR 1080
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
492 LIMK2 3985
Affinity Capture-MS Homo sapiens
493 ARIH1 25820
Two-hybrid Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here