| Experiment ID |
12 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
MHCI
|
Enriched markers |
|
✔
CANX|cytochrome c
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
15478216
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. |
| Authors |
"Mears R, Craven RA, Hanrahan S, Totty N, Upton C, Young SL, Patel P, Selby PJ, Banks RE" |
| Journal name |
PROTEOMICS
|
| Publication year |
2004 |
| Sample |
Melanoma cells |
| Sample name |
MeWo SK-MEL-28 |
| Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
| Flotation density |
1.13-1.20 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [4700 Proteomics Analyzer] Western blotting |