Gene description for SNW1
Gene name SNW domain containing 1
Gene symbol SNW1
Other names/aliases Bx42
NCOA-62
PRPF45
Prp45
SKIIP
SKIP
Species Homo sapiens
 Database cross references - SNW1
ExoCarta ExoCarta_22938
Vesiclepedia VP_22938
Entrez Gene 22938
HGNC 16696
MIM 603055
UniProt Q13573  
 SNW1 identified in exosomes derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for SNW1
Molecular Function
    retinoic acid receptor binding GO:0042974 IDA
    transcription corepressor activity GO:0003714 IDA
    vitamin D receptor binding GO:0042809 IDA
    protein binding GO:0005515 IPI
    SMAD binding GO:0046332 IDA
    transcription coactivator activity GO:0003713 IDA
    Notch binding GO:0005112 IPI
    poly(A) RNA binding GO:0044822 IDA
    nuclear hormone receptor binding GO:0035257 IDA
Biological Process
    viral process GO:0016032 IEA
    transcription, DNA-templated GO:0006351 TAS
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IMP
    cellular response to retinoic acid GO:0071300 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 IDA
    positive regulation of histone H3-K4 methylation GO:0051571 IMP
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    mRNA splicing, via spliceosome GO:0000398 IDA
    positive regulation by host of viral transcription GO:0043923 IMP
    regulation of retinoic acid receptor signaling pathway GO:0048385 IDA
    Notch signaling pathway GO:0007219 TAS
    regulation of vitamin D receptor signaling pathway GO:0070562 IDA
    gene expression GO:0010467 TAS
    positive regulation of vitamin D receptor signaling pathway GO:0070564 IDA
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IMP
    retinoic acid receptor signaling pathway GO:0048384 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 TAS
    positive regulation of neurogenesis GO:0050769 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    positive transcription elongation factor complex b GO:0008024 IDA
    SMAD3-SMAD4 protein complex GO:0071146 IDA
    spliceosomal complex GO:0005681 IDA
    catalytic step 2 spliceosome GO:0071013 IDA
    nucleoplasm GO:0005654 TAS
    nuclear matrix GO:0016363 IDA
    chromatin GO:0000785 IEA
 Experiment description of studies that identified SNW1 in exosomes
1
Experiment ID 426
MISEV standards
✘ EV Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
✘ EV Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 419
MISEV standards
✘ EV Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 419
MISEV standards
✘ EV Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 420
MISEV standards
✘ EV Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 420
MISEV standards
✘ EV Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 434
MISEV standards
✘ EV Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 435
MISEV standards
✘ EV Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
EV Enriched markers
✔
CANX
EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SNW1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PABPN1 8106
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 RB1 5925
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
3 RBL1  
Reconstituted Complex Homo sapiens
4 SMAD2 4087
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 ASCC2  
Two-hybrid Homo sapiens
6 VDR  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 RBPJ 3516
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
8 NOTCH3 4854
Reconstituted Complex Homo sapiens
9 CIR  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
10 HDAC2 3066
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 SND1 27044
Affinity Capture-Western Homo sapiens
12 NOTCH1 4851
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
13 SIN3A  
Reconstituted Complex Homo sapiens
14 Vdr  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
15 SMAD3 4088
Invitro Homo sapiens
Two-hybrid Homo sapiens
16 NCOR2  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
17 RBL2 5934
Reconstituted Complex Homo sapiens
18 SKI 6497
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
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