Gene description for GNA14
Gene name guanine nucleotide binding protein (G protein), alpha 14
Gene symbol GNA14
Other names/aliases -
Species Homo sapiens
 Database cross references - GNA14
ExoCarta ExoCarta_9630
Entrez Gene 9630
HGNC 4382
MIM 604397
UniProt O95837  
 GNA14 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for GNA14
Molecular Function
    GTPase activity GO:0003924 IEA
    GTP binding GO:0005525 IEA
    G-protein coupled receptor binding GO:0001664 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    metal ion binding GO:0046872 IEA
    signal transducer activity GO:0004871 IBA
    protein binding GO:0005515 IPI
Biological Process
    metabolic process GO:0008152 TAS
    signal transduction GO:0007165 TAS
    blood coagulation GO:0007596 TAS
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IBA
    phospholipase C-activating dopamine receptor signaling pathway GO:0060158 IBA
    platelet activation GO:0030168 TAS
Subcellular Localization
    heterotrimeric G-protein complex GO:0005834 TAS
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified GNA14 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNA14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCR1  
Invitro Homo sapiens
Invivo Homo sapiens
2 OPRL1 4987
Affinity Capture-MS Homo sapiens
3 ADRB2  
Invivo Homo sapiens
4 CHRM2  
Invivo Homo sapiens
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