Gene description for TRMT6
Gene name tRNA methyltransferase 6
Gene symbol TRMT6
Other names/aliases CGI-09
GCD10
Gcd10p
Species Homo sapiens
 Database cross references - TRMT6
ExoCarta ExoCarta_51605
Vesiclepedia VP_51605
Entrez Gene 51605
HGNC 20900
UniProt Q9UJA5  
 TRMT6 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TRMT6
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    mRNA processing GO:0006397 IDA
    tRNA methylation GO:0030488 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    tRNA (m1A) methyltransferase complex GO:0031515 IBA
    tRNA (m1A) methyltransferase complex GO:0031515 IPI
 Experiment description of studies that identified TRMT6 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TRMT6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZRANB1 54764
Affinity Capture-MS Homo sapiens
2 ATP6V1B2 526
Co-fractionation Homo sapiens
3 VPS35 55737
Co-fractionation Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 SNW1 22938
Co-fractionation Homo sapiens
6 CKAP5 9793
Affinity Capture-MS Homo sapiens
7 STK24 8428
Co-fractionation Homo sapiens
8 QARS 5859
Co-fractionation Homo sapiens
9 NCSTN 23385
Affinity Capture-MS Homo sapiens
10 FBXL6  
Affinity Capture-MS Homo sapiens
11 MSH2 4436
Co-fractionation Homo sapiens
12 TTC4  
Co-fractionation Homo sapiens
13 NAPA 8775
Co-fractionation Homo sapiens
14 PSME3 10197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ACAA1 30
Affinity Capture-MS Homo sapiens
16 FOXJ1  
Two-hybrid Homo sapiens
17 VPS37C 55048
Affinity Capture-MS Homo sapiens
18 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
19 SRP9 6726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 RDX 5962
Co-fractionation Homo sapiens
21 UTP14A 10813
Co-fractionation Homo sapiens
22 CUL3 8452
Affinity Capture-MS Homo sapiens
23 DCP1A 55802
Affinity Capture-MS Homo sapiens
24 NTNG1  
Affinity Capture-MS Homo sapiens
25 AP1G1 164
Co-fractionation Homo sapiens
26 SSB 6741
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
27 CAND1 55832
Affinity Capture-MS Homo sapiens
28 HNRNPR 10236
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
29 PTK2 5747
Co-fractionation Homo sapiens
30 EPRS 2058
Co-fractionation Homo sapiens
31 SIRT1  
Affinity Capture-MS Homo sapiens
32 MARS 4141
Co-fractionation Homo sapiens
33 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
34 LRRC8E 80131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ZC3H10  
Affinity Capture-MS Homo sapiens
36 KIAA1598 57698
Co-fractionation Homo sapiens
37 DUS3L 56931
Co-fractionation Homo sapiens
38 GRSF1 2926
Proximity Label-MS Homo sapiens
39 TRMT61A 115708
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CAPZB 832
Affinity Capture-MS Homo sapiens
41 FKBP5 2289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 OGFR 11054
Co-fractionation Homo sapiens
43 INPP4B 8821
Co-fractionation Homo sapiens
44 RAPGEF2 9693
Affinity Capture-MS Homo sapiens
45 FYN 2534
Affinity Capture-MS Homo sapiens
46 TLN2 83660
Co-fractionation Homo sapiens
47 DDX5 1655
Co-fractionation Homo sapiens
48 NTRK1 4914
Affinity Capture-MS Homo sapiens
49 IFI16 3428
Affinity Capture-MS Homo sapiens
50 PPARD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 NHP2L1 4809
Co-fractionation Homo sapiens
52 PTGES3 10728
Affinity Capture-MS Homo sapiens
53 USP43  
Affinity Capture-MS Homo sapiens
54 KLHL22 84861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 NETO2  
Affinity Capture-MS Homo sapiens
56 SKIV2L2 23517
Co-fractionation Homo sapiens
57 NMD3 51068
Co-fractionation Homo sapiens
58 DDI2 84301
Co-fractionation Homo sapiens
59 DNAJC17  
Co-fractionation Homo sapiens
60 POU5F1  
Affinity Capture-MS Homo sapiens
61 EIF2B1 1967
Co-fractionation Homo sapiens
62 HHEX  
Two-hybrid Homo sapiens
63 Ncstn 59287
Affinity Capture-MS Mus musculus
64 NANOG  
Affinity Capture-MS Homo sapiens
65 PUS1 80324
Co-fractionation Homo sapiens
66 CBWD1 55871
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 Dlgap4  
Affinity Capture-MS Mus musculus
68 GRIA2  
Affinity Capture-MS Homo sapiens
69 MDM2  
Affinity Capture-MS Homo sapiens
70 OGT 8473
Reconstituted Complex Homo sapiens
71 RAP2A 5911
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TRMT6 is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA modification in the nucleus and cytosol TAS Reactome
tRNA processing TAS Reactome





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