Gene description for PTK2
Gene name protein tyrosine kinase 2
Gene symbol PTK2
Other names/aliases FADK
FAK
FAK1
FRNK
PPP1R71
p125FAK
pp125FAK
Species Homo sapiens
 Database cross references - PTK2
ExoCarta ExoCarta_5747
Entrez Gene 5747
HGNC 9611
MIM 600758
UniProt Q05397  
 PTK2 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for PTK2
Molecular Function
    JUN kinase binding GO:0008432 IDA
    protein kinase activity GO:0004672 TAS
    actin binding GO:0003779 IDA
    signal transducer activity GO:0004871 IEA
    SH2 domain binding GO:0042169 IPI
    ATP binding GO:0005524 IEA
    receptor binding GO:0005102 IBA
    protein tyrosine kinase activity GO:0004713 ISS
    protein kinase binding GO:0019901 IPI
    protein binding GO:0005515 IPI
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IDA
Biological Process
    positive regulation of protein kinase B signaling GO:0051897 IMP
    positive regulation of cell migration GO:0030335 IDA
    establishment of cell polarity GO:0030010 TAS
    regulation of cell proliferation GO:0042127 IMP
    programmed cell death GO:0012501 TAS
    positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:2000060 ISS
    cell adhesion GO:0007155 IBA
    regulation of protein phosphorylation GO:0001932 IGI
    regulation of cell adhesion mediated by integrin GO:0033628 IDA
    negative regulation of axonogenesis GO:0050771 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 ISS
    regulation of cytoskeleton organization GO:0051493 TAS
    regulation of endothelial cell migration GO:0010594 TAS
    regulation of GTPase activity GO:0043087 TAS
    embryo development GO:0009790 TAS
    vasculogenesis GO:0001570 IEA
    regulation of osteoblast differentiation GO:0045667 IMP
    cell migration GO:0016477 IBA
    positive regulation of protein phosphorylation GO:0001934 IMP
    negative regulation of cell-cell adhesion GO:0022408 IDA
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    establishment of nucleus localization GO:0040023 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 IDA
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    innate immune response GO:0045087 TAS
    extracellular matrix organization GO:0030198 IEA
    angiogenesis GO:0001525 TAS
    apoptotic process GO:0006915 TAS
    platelet activation GO:0030168 TAS
    placenta development GO:0001890 TAS
    protein autophosphorylation GO:0046777 IDA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    cytoskeleton organization GO:0007010 IBA
    small GTPase mediated signal transduction GO:0007264 TAS
    microtubule cytoskeleton organization GO:0000226 IEA
    growth hormone receptor signaling pathway GO:0060396 IDA
    netrin-activated signaling pathway GO:0038007 TAS
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IMP
    blood coagulation GO:0007596 TAS
    cell motility GO:0048870 TAS
    positive regulation of cell proliferation GO:0008284 ISS
    epidermal growth factor receptor signaling pathway GO:0007173 IBA
    negative regulation of anoikis GO:2000811 IMP
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 TAS
    regulation of cell shape GO:0008360 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    signal complex assembly GO:0007172 IEA
    axon guidance GO:0007411 TAS
    heart morphogenesis GO:0003007 TAS
    regulation of focal adhesion assembly GO:0051893 TAS
    negative regulation of synapse assembly GO:0051964 IEA
    ephrin receptor signaling pathway GO:0048013 TAS
    neuron migration GO:0001764 IEA
    cell differentiation GO:0030154 IBA
    central nervous system neuron axonogenesis GO:0021955 IEA
    endothelial cell migration GO:0043542 IEA
    negative regulation of organ growth GO:0046621 IEA
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    positive regulation of protein kinase activity GO:0045860 IMP
    regulation of epithelial cell migration GO:0010632 IGI
    regulation of substrate adhesion-dependent cell spreading GO:1900024 IGI
    integrin-mediated signaling pathway GO:0007229 TAS
Subcellular Localization
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    cytosol GO:0005829 TAS
    apical plasma membrane GO:0016324 IEA
    cytoskeleton GO:0005856 TAS
    stress fiber GO:0001725 IDA
    focal adhesion GO:0005925 IDA
    plasma membrane GO:0005886 IDA
    lamellipodium GO:0030027 IEA
    nucleus GO:0005634 IDA
    cell cortex GO:0005938 IEA
    microtubule organizing center GO:0005815 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified PTK2 in exosomes
1
Experiment ID 256
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 258
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 258
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 259
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 260
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PTK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Affinity Capture-MS Homo sapiens
2 PTPRH  
Invivo Homo sapiens
3 TGFB1I1  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
4 GSN 2934
Affinity Capture-Western Homo sapiens
5 FGR 2268
Affinity Capture-Western Homo sapiens
6 PXN  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 PPP1CB 5500
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
8 NCK1 4690
Reconstituted Complex Homo sapiens
9 CRKL 1399
Two-hybrid Homo sapiens
10 APG12L  
Two-hybrid Homo sapiens
11 CXCR4 7852
Affinity Capture-Western Homo sapiens
12 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
13 SHC1 6464
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
14 GSK3B 2932
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
15 CSK 1445
Invivo Homo sapiens
Invitro Homo sapiens
16 TSC2 7249
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 PCSK1  
Affinity Capture-Western Homo sapiens
18 ARHGAP26 23092
Reconstituted Complex Homo sapiens
19 NEDD9 4739
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 ITGB3 3690
Reconstituted Complex Homo sapiens
21 TSC1  
Affinity Capture-Western Homo sapiens
22 BCAR1 9564
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 CD47 961
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
24 PLCG1 5335
Affinity Capture-Western Homo sapiens
25 EFS  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
26 EGFR 1956
Affinity Capture-Western Homo sapiens
27 RB1CC1 9821
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
28 PTK2 5747
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
29 PDGFRB 5159
Affinity Capture-MS Homo sapiens
30 ERBB3 2065
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
31 APC  
Invivo Homo sapiens
32 NCK2 8440
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
33 PTPN11 5781
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
34 GRB7 2886
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
35 PTPN12  
Invivo Homo sapiens
Invitro Homo sapiens
36 TRIP6  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
37 STAT1 6772
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
38 GIT1 28964
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
39 FYN 2534
Invitro Homo sapiens
40 SOCS2  
Two-hybrid Homo sapiens
41 IGF1R 3480
Affinity Capture-Western Homo sapiens
42 PTEN  
Affinity Capture-Western Homo sapiens
43 CD4 920
Affinity Capture-MS Homo sapiens
44 CCR5 1234
Affinity Capture-Western Homo sapiens
45 DOCK1 1793
Affinity Capture-Western Homo sapiens
46 TLN1 7094
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
47 DCC  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
48 ERBB2 2064
Invivo Homo sapiens
49 EPHA2 1969
Affinity Capture-Western Homo sapiens
50 CIB1 10519
Affinity Capture-Western Homo sapiens
51 EZR 7430
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
52 MAPK8IP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
53 SRC 6714
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
54 PIK3R1 5295
Invivo Homo sapiens
55 SYK 6850
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
56 ITGB5 3693
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
57 BMX  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
58 ITGB2 3689
Affinity Capture-MS Homo sapiens
59 JAK2 3717
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
60 IRS1 3667
Two-hybrid Homo sapiens
61 BIN1 274
Invitro Homo sapiens
62 SELE  
Invitro Homo sapiens
63 ITGA4 3676
Affinity Capture-Western Homo sapiens
64 CRK 1398
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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