Gene description for PTPN12
Gene name protein tyrosine phosphatase, non-receptor type 12
Gene symbol PTPN12
Other names/aliases PTP-PEST
PTPG1
Species Homo sapiens
 Database cross references - PTPN12
ExoCarta ExoCarta_5782
Vesiclepedia VP_5782
Entrez Gene 5782
HGNC 9645
MIM 600079
UniProt Q05209  
 PTPN12 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PTPN12
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 IDA
    protein tyrosine phosphatase activity GO:0004725 EXP
    protein tyrosine phosphatase activity GO:0004725 IBA
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IPI
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 IDA
    peptidyl-tyrosine dephosphorylation GO:0035335 IDA
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 IBA
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 IMP
    tissue regeneration GO:0042246 IEA
    cellular response to epidermal growth factor stimulus GO:0071364 IMP
    negative regulation of ERBB signaling pathway GO:1901185 TAS
    negative regulation of platelet-derived growth factor receptor-beta signaling pathway GO:2000587 TAS
Subcellular Localization
    podosome GO:0002102 IEA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 IEA
    cell projection GO:0042995 IEA
 Experiment description of studies that identified PTPN12 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PTPN12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GSN 2934
Affinity Capture-Western Homo sapiens
2 PSG9  
Two-hybrid Homo sapiens
3 ARHGAP10 79658
Proximity Label-MS Homo sapiens
4 CSK 1445
Proximity Label-MS Homo sapiens
5 SMAD5 4090
Two-hybrid Homo sapiens
6 TNS3 64759
Co-fractionation Homo sapiens
7 CGA  
Two-hybrid Homo sapiens
8 AATK  
Two-hybrid Homo sapiens
9 NCK2 8440
Proximity Label-MS Homo sapiens
10 STX16 8675
Two-hybrid Homo sapiens
11 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 SHC1 6464
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 UGP2 7360
Co-fractionation Homo sapiens
14 IFT20 90410
Two-hybrid Homo sapiens
15 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CSH1  
Two-hybrid Homo sapiens
17 PTPN6 5777
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ASAP1 50807
Proximity Label-MS Homo sapiens
19 RAB5A 5868
Proximity Label-MS Homo sapiens
20 NEDD9 4739
Reconstituted Complex Homo sapiens
21 BCAR1 9564
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
22 PACSIN2 11252
Co-fractionation Homo sapiens
23 TEX10 54881
Two-hybrid Homo sapiens
24 LMTK2 22853
Two-hybrid Homo sapiens
25 ERBB4 2066
Two-hybrid Homo sapiens
26 EFS  
Reconstituted Complex Homo sapiens
27 EGFR 1956
PCA Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
28 SORBS2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
29 PTK2 5747
Proximity Label-MS Homo sapiens
30 IGF1R 3480
Two-hybrid Homo sapiens
31 ERBB3 2065
Two-hybrid Homo sapiens
32 ANKS1A 23294
Co-fractionation Homo sapiens
33 DDX58 23586
Affinity Capture-RNA Homo sapiens
34 ZFP2  
Two-hybrid Homo sapiens
35 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
36 LPXN 9404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 ROR2 4920
Two-hybrid Homo sapiens
38 PLOD2 5352
Co-fractionation Homo sapiens
39 BAG6 7917
Two-hybrid Homo sapiens
40 WASF1 8936
Affinity Capture-Western Homo sapiens
41 THAP2  
Two-hybrid Homo sapiens
42 PSTPIP1 9051
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
43 FGFR2 2263
Two-hybrid Homo sapiens
44 DEK 7913
Co-fractionation Homo sapiens
45 NEFM 4741
Affinity Capture-MS Homo sapiens
46 PSTPIP2 9050
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 PXN 5829
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RBM19 9904
Two-hybrid Homo sapiens
50 EPN1 29924
Co-fractionation Homo sapiens
51 PTK2B 2185
Biochemical Activity Homo sapiens
52 TGFB1I1 7041
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
53 DNPEP 23549
Co-fractionation Homo sapiens
54 GANAB 23193
Co-fractionation Homo sapiens
55 FGFR4 2264
Two-hybrid Homo sapiens
56 DYNLL2 140735
Affinity Capture-MS Homo sapiens
57 VASN 114990
Co-fractionation Homo sapiens
58 KDR  
Two-hybrid Homo sapiens
59 NANOG  
Affinity Capture-MS Homo sapiens
60 UTRN 7402
Two-hybrid Homo sapiens
61 JAK2 3717
Affinity Capture-Western Homo sapiens
62 DYNLL1 8655
Affinity Capture-MS Homo sapiens
63 VAPB 9217
Affinity Capture-MS Homo sapiens
64 CSH2  
Two-hybrid Homo sapiens
65 TRIM47 91107
Co-fractionation Homo sapiens
66 PTK7 5754
Two-hybrid Homo sapiens
67 NUSAP1 51203
Co-fractionation Homo sapiens
68 AIFM1 9131
Affinity Capture-MS Homo sapiens
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