Gene description for LMTK2
Gene name lemur tyrosine kinase 2
Gene symbol LMTK2
Other names/aliases AATYK2
BREK
KPI-2
KPI2
LMR2
PPP1R100
cprk
Species Homo sapiens
 Database cross references - LMTK2
ExoCarta ExoCarta_22853
Entrez Gene 22853
HGNC 17880
MIM 610989
UniProt Q8IWU2  
 LMTK2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for LMTK2
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IDA
    protein binding GO:0005515 IPI
    myosin VI binding GO:0070853 IPI
    protein phosphatase inhibitor activity GO:0004864 IDA
    ATP binding GO:0005524 NAS
Biological Process
    protein autophosphorylation GO:0046777 IDA
    peptidyl-serine phosphorylation GO:0018105 IDA
    protein phosphorylation GO:0006468 IDA
    negative regulation of catalytic activity GO:0043086 IDA
    peptidyl-threonine phosphorylation GO:0018107 IDA
    endocytic recycling GO:0032456 IMP
    early endosome to late endosome transport GO:0045022 IMP
    receptor recycling GO:0001881 IMP
    transferrin transport GO:0033572 IMP
Subcellular Localization
    recycling endosome GO:0055037 IDA
    Golgi apparatus GO:0005794 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    early endosome GO:0005769 IDA
    integral component of membrane GO:0016021 IDA
 Experiment description of studies that identified LMTK2 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for LMTK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK5 1020
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 PPP1R2  
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which LMTK2 is involved
No pathways found





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