Experiment description of studies that identified ILKAP in sEVs

1 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for ILKAP

Protein Interactor ExoCarta ID Identification method PubMed Species 1 MAP2K7 5609 Co-fractionation Homo sapiens 2 UBE2H 7328 Affinity Capture-MS Homo sapiens 3 Wdr48   Affinity Capture-MS Mus musculus 4 OGT 8473 Reconstituted Complex Homo sapiens 5 AATK   Two-hybrid Homo sapiens 6 ROR2 4920 Two-hybrid Homo sapiens 7 CNKSR1   Reconstituted Complex Homo sapiens 8 VDAC3 7419 Cross-Linking-MS (XL-MS) Homo sapiens 9 ERBB2 2064 Two-hybrid Homo sapiens 10 ROR1 4919 Two-hybrid Homo sapiens 11 RPS11 6205 Affinity Capture-MS Homo sapiens 12 GSK3B 2932 Co-fractionation Homo sapiens 13 CSK 1445 Co-fractionation Homo sapiens 14 TEK   Two-hybrid Homo sapiens 15 KDR   Two-hybrid Homo sapiens 16 STAT5A 6776 Affinity Capture-MS Homo sapiens 17 PAK1 5058 Co-fractionation Homo sapiens 18 MAPK1 5594 Co-fractionation Homo sapiens 19 TAF4B   Affinity Capture-MS Homo sapiens 20 PDK1 5163 Affinity Capture-MS Homo sapiens 21 DBN1 1627 Affinity Capture-MS Homo sapiens 22 HIST1H2BG 8339 Proximity Label-MS Homo sapiens 23 RNH1 6050 Affinity Capture-MS Homo sapiens 24 PAK2 5062 Co-fractionation Homo sapiens 25 KIT 3815 Two-hybrid Homo sapiens 26 PRPF4 9128 Proximity Label-MS Homo sapiens 27 CERK 64781 Affinity Capture-MS Homo sapiens 28 MAPK3 5595 Co-fractionation Homo sapiens 29 LMTK2 22853 Two-hybrid Homo sapiens 30 ERBB4 2066 Two-hybrid Homo sapiens 31 CBX3 11335 Proximity Label-MS Homo sapiens 32 SMAD4   Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 33 EGFR 1956 Two-hybrid Homo sapiens 34 CHEK2   Co-fractionation Homo sapiens 35 RPL4 6124 Cross-Linking-MS (XL-MS) Homo sapiens 36 OTUB1 55611 Affinity Capture-MS Homo sapiens 37 ERBB3 2065 Two-hybrid Homo sapiens 38 LTN1 26046 Affinity Capture-MS Homo sapiens 39 MKI67   Co-fractionation Homo sapiens 40 PRKDC 5591 Cross-Linking-MS (XL-MS) Homo sapiens 41 MYO1C 4641 Affinity Capture-MS Homo sapiens 42 C21orf59 56683 Affinity Capture-MS Homo sapiens 43 FGFR3 2261 Co-fractionation Homo sapiens 44 PPM1G 5496 Affinity Capture-MS Homo sapiens 45 UFL1 23376 Affinity Capture-MS Homo sapiens 46 EPHA3 2042 Two-hybrid Homo sapiens 47 NAMPT 10135 Co-fractionation Homo sapiens 48 MUSK   Two-hybrid Homo sapiens 49 PRPF3   Proximity Label-MS Homo sapiens 50 PTPRE 5791 Affinity Capture-MS Homo sapiens 51 CASQ1 844 Affinity Capture-MS Homo sapiens 52 RPA3 6119 Proximity Label-MS Homo sapiens 53 LUM 4060 Affinity Capture-MS Homo sapiens 54 RRM1 6240 Cross-Linking-MS (XL-MS) Homo sapiens 55 IGF1R 3480 Two-hybrid Homo sapiens 56 CDK9 1025 Co-fractionation Homo sapiens 57 PTPRH 5794 Affinity Capture-MS Homo sapiens 58 SLX4   Affinity Capture-MS Homo sapiens 59 RPA4   Proximity Label-MS Homo sapiens 60 PARP1 142 Proximity Label-MS Homo sapiens 61 CHST15 51363 Affinity Capture-MS Homo sapiens 62 EPHA2 1969 Two-hybrid Homo sapiens 63 XRCC6 2547 Proximity Label-MS Homo sapiens 64 HGS 9146 Reconstituted Complex Homo sapiens 65 DNAJB1 3337 Co-fractionation Homo sapiens 66 DDRGK1 65992 Affinity Capture-MS Homo sapiens 67 SNRNP27   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 68 ILK 3611 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 69 CUX1 1523 Affinity Capture-MS Homo sapiens 70 FBXO18 84893 Affinity Capture-MS Homo sapiens 71 LMNA 4000 Proximity Label-MS Homo sapiens 72 NDUFS6   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 73 TPM4 7171 Affinity Capture-MS Homo sapiens 74 MYO1B 4430 Affinity Capture-MS Homo sapiens 75 PTK7 5754 Two-hybrid Homo sapiens 76 RPS6KA1 6195 Co-fractionation Homo sapiens View the network

Pathways in which ILKAP is involved

No pathways found