Gene description for FGFR3
Gene name fibroblast growth factor receptor 3
Gene symbol FGFR3
Other names/aliases ACH
CD333
CEK2
HSFGFR3EX
JTK4
Species Homo sapiens
 Database cross references - FGFR3
ExoCarta ExoCarta_2261
Entrez Gene 2261
HGNC 3690
MIM 134934
UniProt P22607  
 FGFR3 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
Urine 25452312    
 Gene ontology annotations for FGFR3
Molecular Function
    ATP binding GO:0005524 IEA
    fibroblast growth factor-activated receptor activity GO:0005007 IMP
    protein tyrosine kinase activity GO:0004713 IDA
    fibroblast growth factor binding GO:0017134 IPI
    protein binding GO:0005515 IPI
Biological Process
    protein autophosphorylation GO:0046777 IDA
    positive regulation of protein ubiquitination GO:0031398 IEA
    endochondral bone growth GO:0003416 TAS
    alveolar secondary septum development GO:0061144 IEA
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 TAS
    positive regulation of endothelial cell proliferation GO:0001938 IEA
    substantia nigra development GO:0021762 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    negative regulation of developmental growth GO:0048640 ISS
    negative regulation of astrocyte differentiation GO:0048712 IEA
    positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway GO:0090080 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    bone mineralization GO:0030282 ISS
    epithelial cell fate commitment GO:0072148 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    bone morphogenesis GO:0060349 TAS
    somatic stem cell maintenance GO:0035019 IEA
    morphogenesis of an epithelium GO:0002009 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    central nervous system myelination GO:0022010 IEA
    positive regulation of MAPK cascade GO:0043410 IMP
    chondrocyte proliferation GO:0035988 TAS
    positive regulation of cell differentiation GO:0045597 IEA
    MAPK cascade GO:0000165 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    positive regulation of tyrosine phosphorylation of Stat3 protein GO:0042517 IMP
    forebrain development GO:0030900 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    fibroblast growth factor receptor apoptotic signaling pathway GO:1902178 IMP
    negative regulation of mitotic nuclear division GO:0045839 IEA
    endochondral ossification GO:0001958 TAS
    digestive tract morphogenesis GO:0048546 IEA
    bone maturation GO:0070977 ISS
    axonogenesis involved in innervation GO:0060385 IEA
    positive regulation of phospholipase activity GO:0010518 IMP
    lens morphogenesis in camera-type eye GO:0002089 IEA
    innate immune response GO:0045087 TAS
    chondrocyte differentiation GO:0002062 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    positive regulation of cell proliferation GO:0008284 IMP
    cochlea development GO:0090102 IEA
    negative regulation of smoothened signaling pathway GO:0045879 IEA
    skeletal system development GO:0001501 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    lens fiber cell development GO:0070307 IEA
    positive regulation of neuron apoptotic process GO:0043525 IEA
    insulin receptor signaling pathway GO:0008286 TAS
    positive regulation of tyrosine phosphorylation of Stat1 protein GO:0042511 IMP
    response to axon injury GO:0048678 IEA
    inner ear receptor cell differentiation GO:0060113 IEA
    cell-cell signaling GO:0007267 IEA
    JAK-STAT cascade GO:0007259 TAS
Subcellular Localization
    integral component of plasma membrane GO:0005887 IDA
    lysosome GO:0005764 IEA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 TAS
    nucleus GO:0005634 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    transport vesicle GO:0030133 IDA
    cell surface GO:0009986 IEA
    extracellular region GO:0005576 IEA
    cytoplasmic side of plasma membrane GO:0009898 IEA
    endoplasmic reticulum GO:0005783 IEA
    focal adhesion GO:0005925 TAS
 Experiment description of studies that identified FGFR3 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 238
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R.
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for FGFR3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CTSK  
Two-hybrid Homo sapiens
2 SLC25A6 293
Two-hybrid Homo sapiens
3 GRB2 2885
Invivo Homo sapiens
4 Sh2bpsm1  
Two-hybrid Mus musculus
5 KRT8 3856
Two-hybrid Homo sapiens
6 GTF3C1  
Two-hybrid Homo sapiens
7 RNF130  
Two-hybrid Homo sapiens
8 SMG7  
Two-hybrid Homo sapiens
9 FLJ10324  
Two-hybrid Homo sapiens
10 KIAA1377  
Two-hybrid Homo sapiens
11 FLJ22624  
Two-hybrid Homo sapiens
12 CCDC17  
Two-hybrid Homo sapiens
13 POLA2  
Two-hybrid Homo sapiens
14 C6orf47  
Two-hybrid Homo sapiens
15 FGF9  
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
16 HNRNPL 3191
Two-hybrid Homo sapiens
17 FGF8  
Invivo Homo sapiens
18 RPL8 6132
Two-hybrid Homo sapiens
19 SH2B1 25970
Affinity Capture-Western Homo sapiens
20 HBZ  
Two-hybrid Homo sapiens
21 CHGB  
Two-hybrid Homo sapiens
22 ATF3  
Two-hybrid Homo sapiens
23 NDUFS6  
Two-hybrid Homo sapiens
24 FGF1  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
25 ARAP1 116985
Two-hybrid Homo sapiens
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