Gene description for C1QB
Gene name complement component 1, q subcomponent, B chain
Gene symbol C1QB
Other names/aliases -
Species Homo sapiens
 Database cross references - C1QB
ExoCarta ExoCarta_713
Vesiclepedia VP_713
Entrez Gene 713
HGNC 1242
MIM 120570
UniProt P02746  
 C1QB identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Malignant pleural effusions 14975938    
Malignant pleural effusions 14975938    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for C1QB
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    complement activation GO:0006956 TAS
    complement activation, classical pathway GO:0006958 IDA
    complement activation, classical pathway GO:0006958 NAS
    innate immune response GO:0045087 IEA
    synapse pruning GO:0098883 ISS
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    collagen trimer GO:0005581 IEA
    complement component C1 complex GO:0005602 NAS
    synapse GO:0045202 ISS
    collagen-containing extracellular matrix GO:0062023 HDA
    complement component C1q complex GO:0062167 IPI
    blood microparticle GO:0072562 HDA
    postsynapse GO:0098794 ISS
 Experiment description of studies that identified C1QB in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 52
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Lung cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
4
Experiment ID 53
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Mesothelioma
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
5
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for C1QB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 COLGALT2  
Affinity Capture-MS Homo sapiens
3 RELA 5970
Two-hybrid Homo sapiens
4 METRN 79006
Affinity Capture-MS Homo sapiens
5 TRHDE 29953
Affinity Capture-MS Homo sapiens
6 NAAA  
Affinity Capture-MS Homo sapiens
7 LRFN3  
Affinity Capture-MS Homo sapiens
8 COL4A2 1284
Affinity Capture-MS Homo sapiens
9 CNTNAP3  
Affinity Capture-MS Homo sapiens
10 P3H4 10609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 MYCBP2 23077
Affinity Capture-MS Homo sapiens
12 METRNL 284207
Affinity Capture-MS Homo sapiens
13 FOXRED2  
Affinity Capture-MS Homo sapiens
14 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
15 ADAMTS7  
Affinity Capture-MS Homo sapiens
16 SEMA6B 10501
Affinity Capture-MS Homo sapiens
17 IL32  
Two-hybrid Homo sapiens
18 UBQLN2 29978
Two-hybrid Homo sapiens
19 BMP1 649
Affinity Capture-MS Homo sapiens
20 CERCAM 51148
Affinity Capture-MS Homo sapiens
21 B3GNT3 10331
Affinity Capture-MS Homo sapiens
22 GNG8  
Affinity Capture-MS Homo sapiens
23 MYOC 4653
Two-hybrid Homo sapiens
24 ATP12A 479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CELSR1 9620
Affinity Capture-MS Homo sapiens
26 LOXL2 4017
Affinity Capture-MS Homo sapiens
27 OLFM2  
Affinity Capture-MS Homo sapiens
28 CHST14 113189
Affinity Capture-MS Homo sapiens
29 INSR 3643
Affinity Capture-MS Homo sapiens
30 DMWD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 VHL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 BDNF 627
Affinity Capture-MS Homo sapiens
33 CLSTN1 22883
Affinity Capture-MS Homo sapiens
34 COL18A1 80781
Affinity Capture-MS Homo sapiens
35 NAGLU 4669
Affinity Capture-MS Homo sapiens
36 FN1 2335
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 COL6A2 1292
Affinity Capture-MS Homo sapiens
38 FGFR3 2261
Affinity Capture-MS Homo sapiens
39 UFL1 23376
Affinity Capture-MS Homo sapiens
40 MGAT5 4249
Affinity Capture-MS Homo sapiens
41 MANBA 4126
Affinity Capture-MS Homo sapiens
42 USP30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 PLOD2 5352
Affinity Capture-MS Homo sapiens
44 VWDE  
Affinity Capture-MS Homo sapiens
45 EDA2R  
Two-hybrid Homo sapiens
46 CHRNA5  
Affinity Capture-MS Homo sapiens
47 TMEM132A 54972
Affinity Capture-MS Homo sapiens
48 MAN2B2 23324
Affinity Capture-MS Homo sapiens
49 MAN2A2 4122
Affinity Capture-MS Homo sapiens
50 CHPF2 54480
Affinity Capture-MS Homo sapiens
51 PCSK9 255738
Affinity Capture-MS Homo sapiens
52 COL12A1 1303
Affinity Capture-MS Homo sapiens
53 PINK1  
Affinity Capture-MS Homo sapiens
54 GUSB 2990
Affinity Capture-MS Homo sapiens
55 NLGN2 57555
Affinity Capture-MS Homo sapiens
56 BMP8A  
Affinity Capture-MS Homo sapiens
57 C6orf120  
Affinity Capture-MS Homo sapiens
58 GK  
Affinity Capture-MS Homo sapiens
59 METAP2 10988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 PCSK6 5046
Affinity Capture-MS Homo sapiens
61 HYAL2 8692
Affinity Capture-MS Homo sapiens
62 P3H3 10536
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 DDRGK1 65992
Affinity Capture-MS Homo sapiens
64 FGFR4 2264
Affinity Capture-MS Homo sapiens
65 NAGA 4668
Affinity Capture-MS Homo sapiens
66 ART5  
Affinity Capture-MS Homo sapiens
67 PTPRU  
Affinity Capture-MS Homo sapiens
68 PLXNA3 55558
Affinity Capture-MS Homo sapiens
69 OBSL1 23363
Affinity Capture-MS Homo sapiens
70 ARSK  
Affinity Capture-MS Homo sapiens
71 CCR1  
Affinity Capture-MS Homo sapiens
72 CBWD1 55871
Affinity Capture-MS Homo sapiens
73 GAS6 2621
Affinity Capture-MS Homo sapiens
74 LAMA5 3911
Affinity Capture-MS Homo sapiens
75 PLXNA1 5361
Affinity Capture-MS Homo sapiens
76 UBE2U  
Affinity Capture-MS Homo sapiens
77 CCRN4L  
Affinity Capture-MS Homo sapiens
78 EED  
Affinity Capture-MS Homo sapiens
79 MAN2B1 4125
Affinity Capture-MS Homo sapiens
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