Gene description for GUSB
Gene name glucuronidase, beta
Gene symbol GUSB
Other names/aliases BG
MPS7
Species Homo sapiens
 Database cross references - GUSB
ExoCarta ExoCarta_2990
Entrez Gene 2990
HGNC 4696
MIM 611499
UniProt P08236  
 GUSB identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 25452312    
 Gene ontology annotations for GUSB
Molecular Function
    beta-glucuronidase activity GO:0004566 TAS
    protein domain specific binding GO:0019904 IPI
    receptor binding GO:0005102 IPI
Biological Process
    hyaluronan metabolic process GO:0030212 TAS
    small molecule metabolic process GO:0044281 TAS
    hyaluronan catabolic process GO:0030214 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    carbohydrate metabolic process GO:0005975 TAS
    glycosaminoglycan catabolic process GO:0006027 TAS
Subcellular Localization
    membrane GO:0016020 IDA
    extracellular exosome GO:0070062 IDA
    lysosomal lumen GO:0043202 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified GUSB in exosomes
1
Experiment ID 13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 238
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R.
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GUSB
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which GUSB is involved
PathwayEvidenceSource
HS-GAG degradation TAS Reactome
Hyaluronan uptake and degradation TAS Reactome
MPS VII - Sly syndrome TAS Reactome





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