Gene description for CHST14
Gene name carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14
Gene symbol CHST14
Other names/aliases ATCS
D4ST1
EDSMC1
HNK1ST
Species Homo sapiens
 Database cross references - CHST14
ExoCarta ExoCarta_113189
Entrez Gene 113189
HGNC 24464
MIM 608429
UniProt Q8NCH0  
 CHST14 identified in exosomes derived from the following tissue/cell type
Saliva 19199708    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for CHST14
Molecular Function
    phosphate ion binding GO:0042301 NAS
    N-acetylgalactosamine 4-O-sulfotransferase activity GO:0001537 IDA
Biological Process
    carbohydrate biosynthetic process GO:0016051 IEA
    dermatan sulfate biosynthetic process GO:0030208 TAS
    carbohydrate metabolic process GO:0005975 TAS
    dermatan sulfate proteoglycan metabolic process GO:0050655 IDA
    glycosaminoglycan metabolic process GO:0030203 TAS
    chondroitin sulfate metabolic process GO:0030204 TAS
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    integral component of membrane GO:0016021 NAS
    Golgi membrane GO:0000139 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CHST14 in exosomes
1
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CHST14
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which CHST14 is involved
PathwayEvidenceSource
Defective CHST14 causes EDS, musculocontractural type TAS Reactome
Dermatan sulfate biosynthesis TAS Reactome





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