Gene description for HYAL2
Gene name hyaluronoglucosaminidase 2
Gene symbol HYAL2
Other names/aliases LUCA2
Species Homo sapiens
 Database cross references - HYAL2
ExoCarta ExoCarta_8692
Vesiclepedia VP_8692
Entrez Gene 8692
HGNC 5321
MIM 603551
UniProt Q12891  
 HYAL2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for HYAL2
Molecular Function
    transforming growth factor beta binding GO:0050431 ISS
    hyaluronoglucuronidase activity GO:0033906 IDA
    enzyme binding GO:0019899 IPI
    receptor signaling protein tyrosine kinase inhibitor activity GO:0030294 IDA
    virus receptor activity GO:0001618 IDA
    hyalurononglucosaminidase activity GO:0004415 TAS
    protein binding GO:0005515 IPI
    receptor tyrosine kinase binding GO:0030971 IPI
    hyaluronic acid binding GO:0005540 IDA
Biological Process
    cellular response to UV-B GO:0071493 IDA
    viral entry into host cell GO:0046718 ISS
    glycosaminoglycan catabolic process GO:0006027 IDA
    response to antibiotic GO:0046677 IEP
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    cellular response to tumor necrosis factor GO:0071356 IEP
    hyaluronan catabolic process GO:0030214 TAS
    negative regulation of protein tyrosine kinase activity GO:0061099 IDA
    negative regulation of protein kinase B signaling GO:0051898 IDA
    negative regulation of fibroblast migration GO:0010764 IDA
    kidney development GO:0001822 ISS
    monocyte activation GO:0042117 IDA
    cellular response to transforming growth factor beta stimulus GO:0071560 IDA
    cellular response to interleukin-1 GO:0071347 IDA
    glycosaminoglycan metabolic process GO:0030203 TAS
    skeletal system morphogenesis GO:0048705 IEA
    transformation of host cell by virus GO:0019087 ISS
    hyaluronan metabolic process GO:0030212 TAS
    response to reactive oxygen species GO:0000302 IDA
    positive regulation of protein import into nucleus GO:0042307 ISS
    multicellular organismal aging GO:0010259 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISS
    fusion of virus membrane with host plasma membrane GO:0019064 IDA
    defense response to virus GO:0051607 IEA
    positive regulation of inflammatory response GO:0050729 IDA
    small molecule metabolic process GO:0044281 TAS
    negative regulation of MAP kinase activity GO:0043407 IDA
    response to virus GO:0009615 IDA
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 ISS
    positive regulation of interleukin-8 secretion GO:2000484 IDA
    positive regulation of interleukin-6 secretion GO:2000778 IDA
    positive regulation of urine volume GO:0035810 ISS
    multicellular organismal iron ion homeostasis GO:0060586 IEA
    renal water absorption GO:0070295 ISS
    negative regulation of cell growth GO:0030308 IDA
    cartilage development GO:0051216 IEP
    cellular response to fibroblast growth factor stimulus GO:0044344 IDA
    carbohydrate metabolic process GO:0005975 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    anchored component of external side of plasma membrane GO:0031362 IDA
    Golgi membrane GO:0000139 ISS
    cytoplasm GO:0005737 IDA
    RNA polymerase II transcription factor complex GO:0090575 ISS
    perinuclear region of cytoplasm GO:0048471 IDA
    endoplasmic reticulum GO:0005783 NAS
    microvillus GO:0005902 IDA
    endocytic vesicle GO:0030139 IDA
    membrane raft GO:0045121 IDA
    lysosome GO:0005764 IDA
    cytoplasmic membrane-bounded vesicle GO:0016023 IDA
    cell surface GO:0009986 ISS
    cytoplasmic vesicle GO:0031410 IDA
    apical plasma membrane GO:0016324 IDA
    anchored component of plasma membrane GO:0046658 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified HYAL2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HYAL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ERVWE1  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which HYAL2 is involved
PathwayEvidenceSource
Hyaluronan uptake and degradation TAS Reactome





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