Experiment description of studies that identified DPEP1 in exosomes |
1 |
Experiment ID |
21 |
ISEV standards |
✔
EM|IEM
|
EV Biophysical techniques |
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
✔
CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
19837982
|
Organism |
Homo sapiens |
Experiment description |
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. |
Authors |
Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson |
Journal name |
MCP
|
Publication year |
2009 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1215 |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.10-1.12 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [Orbitrap] Western blotting |
|
|
2 |
Experiment ID |
282 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
3 |
Experiment ID |
283 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
4 |
Experiment ID |
285 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
5 |
Experiment ID |
286 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
6 |
Experiment ID |
4 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63|MHCI|MHCII
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
11487543
|
Organism |
Homo sapiens |
Experiment description |
Intestinal epithelial cells secrete exosome-like vesicles. |
Authors |
van Niel G, Raposo G, Candalh C, Boussac M, Hershberg R, Cerf-Bensussan N, Heyman M |
Journal name |
|
Publication year |
2001 |
Sample |
Intestinal epithelial cells |
Sample name |
HT29-19A T84-DRB1*0401/CIITA |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF] Western blotting |
|
|
7 |
Experiment ID |
13 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
Alix|RAB4|RAB5B|RAB11|TSG101
|
EV Cytosolic markers |
✔
CD9|AQP2|AQP1
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
15326289
|
Organism |
Homo sapiens |
Experiment description |
Identification and proteomic profiling of exosomes in human urine. |
Authors |
Pisitkun T, Shen RF, Knepper MA |
Journal name |
PNAS
|
Publication year |
2004 |
Sample |
Urine |
Sample name |
Urine - Normal |
Isolation/purification methods |
Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LCQ DECA XP] Western blotting |
|
|
8 |
Experiment ID |
63 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
AQP2
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19056867
|
Organism |
Homo sapiens |
Experiment description |
Large-scale proteomics and phosphoproteomics of urinary exosomes. |
Authors |
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA |
Journal name |
JASN
|
Publication year |
2009 |
Sample |
Urine |
Sample name |
Urine - Normal |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting |
|
|
9 |
Experiment ID |
193 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63|CD9
|
EV Membrane markers |
✔
PHB
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21595033
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. |
Authors |
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC. |
Journal name |
Proteomics
|
Publication year |
2011 |
Sample |
Urine |
Sample name |
Urine - Normal |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
10 |
Experiment ID |
194 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21595033
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. |
Authors |
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC. |
Journal name |
Proteomics
|
Publication year |
2011 |
Sample |
Urine |
Sample name |
Urine - Patients of basement membrane nephropathy |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
11 |
Experiment ID |
195 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21595033
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. |
Authors |
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC. |
Journal name |
Proteomics
|
Publication year |
2011 |
Sample |
Urine |
Sample name |
Urine - Patients of early IgA nephropathy |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
12 |
Experiment ID |
196 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
22418980
|
Organism |
Homo sapiens |
Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
Authors |
Raj DA, Fiume I, Capasso G, Pocsfalvi G. |
Journal name |
Kidney Int
|
Publication year |
2012 |
Sample |
Urine |
Sample name |
Urine - Normal high density |
Isolation/purification methods |
Differential centrifugation Sucrose cushion |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
13 |
Experiment ID |
197 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
22418980
|
Organism |
Homo sapiens |
Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
Authors |
Raj DA, Fiume I, Capasso G, Pocsfalvi G. |
Journal name |
Kidney Int
|
Publication year |
2012 |
Sample |
Urine |
Sample name |
Urine - Normal low density |
Isolation/purification methods |
Differential centrifugation Sucrose cushion |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
14 |
Experiment ID |
238 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25452312
|
Organism |
Homo sapiens |
Experiment description |
N-linked (N-) glycoproteomics of urimary exosomes |
Authors |
Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2014 |
Sample |
Urine |
Sample name |
Urine - Normal |
Isolation/purification methods |
Differential centrifugation Filtration Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|