Gene description for GPAA1
Gene name glycosylphosphatidylinositol anchor attachment 1
Gene symbol GPAA1
Other names/aliases GAA1
hGAA1
Species Homo sapiens
 Database cross references - GPAA1
ExoCarta ExoCarta_8733
Vesiclepedia VP_8733
Entrez Gene 8733
HGNC 4446
MIM 603048
UniProt O43292  
 GPAA1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for GPAA1
Molecular Function
    GPI-anchor transamidase activity GO:0003923 NAS
    protein binding GO:0005515 IPI
    GPI anchor binding GO:0034235 IMP
Biological Process
    GPI anchor biosynthetic process GO:0006506 IEA
    protein retention in ER lumen GO:0006621 NAS
    attachment of GPI anchor to protein GO:0016255 IBA
    attachment of GPI anchor to protein GO:0016255 IMP
    attachment of GPI anchor to protein GO:0016255 NAS
    attachment of GPI anchor to protein GO:0016255 TAS
Subcellular Localization
    mitochondrion GO:0005739 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    GPI-anchor transamidase complex GO:0042765 IBA
    GPI-anchor transamidase complex GO:0042765 IDA
    GPI-anchor transamidase complex GO:0042765 IPI
    GPI-anchor transamidase complex GO:0042765 TAS
 Experiment description of studies that identified GPAA1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GPAA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STARD3NL 83930
Affinity Capture-MS Homo sapiens
2 PTGIR  
Affinity Capture-MS Homo sapiens
3 GABRE  
Affinity Capture-MS Homo sapiens
4 HTR3A  
Affinity Capture-MS Homo sapiens
5 GNRHR  
Two-hybrid Homo sapiens
6 HS2ST1 9653
Affinity Capture-MS Homo sapiens
7 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
10 AQP2 359
Two-hybrid Homo sapiens
11 VNN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 VAPB 9217
Affinity Capture-MS Homo sapiens
13 BTNL2  
Affinity Capture-MS Homo sapiens
14 FAM20C 56975
Affinity Capture-MS Homo sapiens
15 TREH 11181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PTP4A2 8073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 NDUFS1 4719
Affinity Capture-MS Homo sapiens
18 GP9 2815
Affinity Capture-MS Homo sapiens
19 SYNGR1 9145
Affinity Capture-MS Homo sapiens
20 RTP2  
Affinity Capture-MS Homo sapiens
21 GABRA3  
Affinity Capture-MS Homo sapiens
22 TCTN3  
Affinity Capture-MS Homo sapiens
23 PIN1 5300
Two-hybrid Homo sapiens
24 PSCA 8000
Affinity Capture-MS Homo sapiens
25 MCOLN3  
Affinity Capture-MS Homo sapiens
26 HTR3C  
Affinity Capture-MS Homo sapiens
27 MOXD1 26002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CRELD1 78987
Affinity Capture-MS Homo sapiens
29 TMEM95  
Affinity Capture-MS Homo sapiens
30 PRSS50  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TSPAN15 23555
Affinity Capture-MS Homo sapiens
32 B3GNT3 10331
Affinity Capture-MS Homo sapiens
33 ARF6 382
Affinity Capture-MS Homo sapiens
34 IFI6  
Affinity Capture-MS Homo sapiens
35 PIGK 10026
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
36 DPEP1 1800
Affinity Capture-MS Homo sapiens
37 PIGU 128869
Co-fractionation Homo sapiens
38 ART4  
Affinity Capture-MS Homo sapiens
39 SPPL2B 56928
Affinity Capture-MS Homo sapiens
40 Rock1  
Affinity Capture-MS Mus musculus
41 STOM 2040
Affinity Capture-MS Homo sapiens
42 DDX58 23586
Affinity Capture-RNA Homo sapiens
43 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 AP1G2 8906
Affinity Capture-MS Homo sapiens
45 EIF3E 3646
Two-hybrid Homo sapiens
46 GPR173  
Affinity Capture-MS Homo sapiens
47 SCN4A  
Affinity Capture-MS Homo sapiens
48 GABRG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 EMR4P  
Affinity Capture-MS Homo sapiens
50 PARK2  
Affinity Capture-MS Homo sapiens
51 TSPAN8 7103
Affinity Capture-MS Homo sapiens
52 SOAT1 6646
Affinity Capture-MS Homo sapiens
53 CD7  
Affinity Capture-MS Homo sapiens
54 RPA3 6119
Proximity Label-MS Homo sapiens
55 FBXO2 26232
Affinity Capture-MS Homo sapiens
56 ATP5B 506
Affinity Capture-MS Homo sapiens
57 ACAP2 23527
Affinity Capture-MS Homo sapiens
58 PIGT 51604
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 EFNA4  
Affinity Capture-MS Homo sapiens
60 GPR182  
Affinity Capture-MS Homo sapiens
61 PRSS8 5652
Affinity Capture-MS Homo sapiens
62 SMPD2 6610
Affinity Capture-MS Homo sapiens
63 P2RY2 5029
Affinity Capture-MS Homo sapiens
64 ENTPD2 954
Affinity Capture-MS Homo sapiens
65 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 GPRC5B 51704
Affinity Capture-MS Homo sapiens
67 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
68 Ptpn13  
Affinity Capture-MS Mus musculus
69 AGTR1  
Two-hybrid Homo sapiens
70 CCDC47 57003
Affinity Capture-MS Homo sapiens
71 THY1 7070
Affinity Capture-MS Homo sapiens
72 FPR2  
Affinity Capture-MS Homo sapiens
73 KCNK16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 TTYH1  
Affinity Capture-MS Homo sapiens
75 ZACN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 PIGS 94005
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
77 CACNA2D3  
Affinity Capture-MS Homo sapiens
78 COX4I1 1327
Affinity Capture-MS Homo sapiens
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