Gene description for SMPD2
Gene name sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)
Gene symbol SMPD2
Other names/aliases ISC1
NSMASE
NSMASE1
Species Homo sapiens
 Database cross references - SMPD2
ExoCarta ExoCarta_6610
Vesiclepedia VP_6610
Entrez Gene 6610
HGNC 11121
MIM 603498
UniProt O60906  
 SMPD2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SMPD2
Molecular Function
    sphingomyelin phosphodiesterase activity GO:0004767 IBA
    sphingomyelin phosphodiesterase activity GO:0004767 IDA
    protein binding GO:0005515 IPI
    phosphoric diester hydrolase activity GO:0008081 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    sphingomyelin metabolic process GO:0006684 IBA
    sphingomyelin catabolic process GO:0006685 IDA
    sphingolipid catabolic process GO:0030149 IBA
    ceramide biosynthetic process GO:0046513 IBA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    caveola GO:0005901 IBA
    cell periphery GO:0071944 IBA
 Experiment description of studies that identified SMPD2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMPD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PGRMC2 10424
Two-hybrid Homo sapiens
2 NKAIN1  
Affinity Capture-MS Homo sapiens
3 REEP5 7905
Affinity Capture-MS Homo sapiens
4 MTHFR 4524
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SLC30A9 10463
Affinity Capture-MS Homo sapiens
6 FAM189B  
Affinity Capture-MS Homo sapiens
7 KCNMB3  
Affinity Capture-MS Homo sapiens
8 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
9 EDNRA 1909
Affinity Capture-MS Homo sapiens
10 PCDHB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 PIGS 94005
Affinity Capture-MS Homo sapiens
12 GAD2  
Two-hybrid Homo sapiens
13 RPN2 6185
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 RXFP1  
Affinity Capture-MS Homo sapiens
15 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
16 PCDHGA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ADCK3  
Two-hybrid Homo sapiens
18 RTN3 10313
Affinity Capture-MS Homo sapiens
19 SRPRB 58477
Affinity Capture-MS Homo sapiens
20 TEX28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TMED5 50999
Affinity Capture-MS Homo sapiens
22 PCDHGB4 8641
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PITPNC1  
Two-hybrid Homo sapiens
24 KCNC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MANSC1 54682
Affinity Capture-MS Homo sapiens
26 IL22RA1 58985
Affinity Capture-MS Homo sapiens
27 STAR  
Two-hybrid Homo sapiens
28 TMEM14B  
Two-hybrid Homo sapiens
29 NMUR2  
Affinity Capture-MS Homo sapiens
30 TMED6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 HSD17B11 51170
Proximity Label-MS Homo sapiens
32 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
33 PCDHA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CIB1 10519
Affinity Capture-MS Homo sapiens
35 OR10H2  
Affinity Capture-MS Homo sapiens
36 LRRC32 2615
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 SCAP  
Affinity Capture-MS Homo sapiens
38 ZDHHC12  
Affinity Capture-MS Homo sapiens
39 ARFIP1 27236
Two-hybrid Homo sapiens
40 PCDHGB2  
Affinity Capture-MS Homo sapiens
41 NDRG4 65009
Two-hybrid Homo sapiens
42 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
43 CD300C  
Affinity Capture-MS Homo sapiens
44 PCDHA11  
Affinity Capture-MS Homo sapiens
45 PBX3  
Two-hybrid Homo sapiens
46 PCDHGC4  
Affinity Capture-MS Homo sapiens
47 PLVAP 83483
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PCK1 5105
Affinity Capture-MS Homo sapiens
50 SLC22A2 6582
Affinity Capture-MS Homo sapiens
51 SLC10A1  
Two-hybrid Homo sapiens
52 LRRTM1 347730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 HARS2 23438
Affinity Capture-MS Homo sapiens
54 MARCH4  
Affinity Capture-MS Homo sapiens
55 TMED2 10959
Affinity Capture-MS Homo sapiens
56 PCDHB16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 FNTB 2342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 ANAPC10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 TMEM30A 55754
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 CDY2A  
Affinity Capture-MS Homo sapiens
61 TMEM31  
Two-hybrid Homo sapiens
62 GRIN2A  
Affinity Capture-MS Homo sapiens
63 GPAA1 8733
Affinity Capture-MS Homo sapiens
64 CYR61 3491
Two-hybrid Homo sapiens
65 LAMP5  
Affinity Capture-MS Homo sapiens
66 TMED7 51014
Affinity Capture-MS Homo sapiens
67 RTN4 57142
Affinity Capture-MS Homo sapiens
68 CD1B 910
Affinity Capture-MS Homo sapiens
69 C3orf52  
Affinity Capture-MS Homo sapiens
70 TTYH1  
Affinity Capture-MS Homo sapiens
71 PCDHAC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 TMEM167B  
Two-hybrid Homo sapiens
View the network image/svg+xml



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