Gene description for TMED5
Gene name transmembrane emp24 protein transport domain containing 5
Gene symbol TMED5
Other names/aliases CGI-100
p28
Species Homo sapiens
 Database cross references - TMED5
ExoCarta ExoCarta_50999
Vesiclepedia VP_50999
Entrez Gene 50999
HGNC 24251
UniProt Q9Y3A6  
 TMED5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TMED5
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    Golgi ribbon formation GO:0090161 IBA
    Golgi ribbon formation GO:0090161 IMP
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IBA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IDA
    Golgi apparatus GO:0005794 IBA
    cis-Golgi network GO:0005801 IDA
    COPII-coated ER to Golgi transport vesicle GO:0030134 IBA
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 IEA
    endoplasmic reticulum exit site GO:0070971 IDA
 Experiment description of studies that identified TMED5 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TMED5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF622 90441
Affinity Capture-MS Homo sapiens
2 Tmed2 56334
Affinity Capture-MS Mus musculus
3 TMED10 10972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
4 KLK15  
Affinity Capture-MS Homo sapiens
5 VAPA 9218
Affinity Capture-MS Homo sapiens
6 TMED9 54732
Affinity Capture-MS Homo sapiens
7 DAD1 1603
Affinity Capture-MS Homo sapiens
8 CANX 821
Affinity Capture-MS Homo sapiens
9 Tmed10 68581
Affinity Capture-MS Mus musculus
10 TSC2 7249
Affinity Capture-MS Homo sapiens
11 EMC1 23065
Affinity Capture-MS Homo sapiens
12 POTEI 653269
Affinity Capture-MS Homo sapiens
13 TMED4 222068
Affinity Capture-MS Homo sapiens
14 CHST8 64377
Affinity Capture-MS Homo sapiens
15 EMC7 56851
Affinity Capture-MS Homo sapiens
16 CD70 970
Affinity Capture-MS Homo sapiens
17 NFKBIL1  
Affinity Capture-MS Homo sapiens
18 PRMT6  
Two-hybrid Homo sapiens
19 PTP4A1 7803
Affinity Capture-MS Homo sapiens
20 ECI1 1632
Cross-Linking-MS (XL-MS) Homo sapiens
21 TSC1 7248
Affinity Capture-MS Homo sapiens
22 COPE 11316
Affinity Capture-MS Homo sapiens
23 YIPF5 81555
Affinity Capture-MS Homo sapiens
24 COPA 1314
Affinity Capture-MS Homo sapiens
25 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
26 FN1 2335
Affinity Capture-MS Homo sapiens
27 PDE2A  
Affinity Capture-MS Homo sapiens
28 KIAA1429 25962
Affinity Capture-MS Homo sapiens
29 BRI3BP 140707
Affinity Capture-MS Homo sapiens
30 LHX1  
Affinity Capture-MS Homo sapiens
31 HOOK1  
Proximity Label-MS Homo sapiens
32 SREBF2 6721
Affinity Capture-MS Homo sapiens
33 RPA3 6119
Proximity Label-MS Homo sapiens
34 GOLGB1 2804
Affinity Capture-MS Homo sapiens
35 S1PR1 1901
Affinity Capture-MS Homo sapiens
36 GPR45  
Affinity Capture-MS Homo sapiens
37 PLEKHD1  
Cross-Linking-MS (XL-MS) Homo sapiens
38 KIF14 9928
Affinity Capture-MS Homo sapiens
39 CLTA 1211
Affinity Capture-MS Homo sapiens
40 GPR182  
Affinity Capture-MS Homo sapiens
41 COPB2 9276
Affinity Capture-MS Homo sapiens
42 CDK5RAP3 80279
Affinity Capture-MS Homo sapiens
43 SMPD2 6610
Affinity Capture-MS Homo sapiens
44 PRC1 9055
Affinity Capture-MS Homo sapiens
45 TMED2 10959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CXCL9  
Two-hybrid Homo sapiens
47 DDRGK1 65992
Affinity Capture-MS Homo sapiens
48 RNF13 11342
Affinity Capture-MS Homo sapiens
49 FGFRL1 53834
Affinity Capture-MS Homo sapiens
50 VIPR1  
Affinity Capture-MS Homo sapiens
51 ATP13A3 79572
Affinity Capture-MS Homo sapiens
52 TMEM132D  
Two-hybrid Homo sapiens
53 ATP2A3 489
Affinity Capture-MS Homo sapiens
54 PPP1R13B  
Affinity Capture-MS Homo sapiens
55 PRKRIR  
Affinity Capture-MS Homo sapiens
56 HERC5 51191
Affinity Capture-MS Homo sapiens
57 GNL3L 54552
Cross-Linking-MS (XL-MS) Homo sapiens
58 LIPG 9388
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMED5 is involved
PathwayEvidenceSource
Signal Transduction TAS Reactome
Signaling by WNT TAS Reactome
WNT ligand biogenesis and trafficking TAS Reactome





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