Gene ontology annotations for TMED5
Experiment description of studies that identified TMED5 in exosomes
1
Experiment ID
489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
6
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
8
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
9
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for TMED5
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
ZNF622
90441
Affinity Capture-MS
Homo sapiens
2
Tmed2
56334
Affinity Capture-MS
Mus musculus
3
TMED10
10972
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
4
KLK15
Affinity Capture-MS
Homo sapiens
5
VAPA
9218
Affinity Capture-MS
Homo sapiens
6
TMED9
54732
Affinity Capture-MS
Homo sapiens
7
DAD1
1603
Affinity Capture-MS
Homo sapiens
8
CANX
821
Affinity Capture-MS
Homo sapiens
9
Tmed10
68581
Affinity Capture-MS
Mus musculus
10
TSC2
7249
Affinity Capture-MS
Homo sapiens
11
EMC1
23065
Affinity Capture-MS
Homo sapiens
12
POTEI
653269
Affinity Capture-MS
Homo sapiens
13
TMED4
222068
Affinity Capture-MS
Homo sapiens
14
CHST8
64377
Affinity Capture-MS
Homo sapiens
15
EMC7
56851
Affinity Capture-MS
Homo sapiens
16
CD70
970
Affinity Capture-MS
Homo sapiens
17
NFKBIL1
Affinity Capture-MS
Homo sapiens
18
PRMT6
Two-hybrid
Homo sapiens
19
PTP4A1
7803
Affinity Capture-MS
Homo sapiens
20
ECI1
1632
Cross-Linking-MS (XL-MS)
Homo sapiens
21
TSC1
7248
Affinity Capture-MS
Homo sapiens
22
COPE
11316
Affinity Capture-MS
Homo sapiens
23
YIPF5
81555
Affinity Capture-MS
Homo sapiens
24
COPA
1314
Affinity Capture-MS
Homo sapiens
25
MYBBP1A
10514
Affinity Capture-MS
Homo sapiens
26
FN1
2335
Affinity Capture-MS
Homo sapiens
27
PDE2A
Affinity Capture-MS
Homo sapiens
28
KIAA1429
25962
Affinity Capture-MS
Homo sapiens
29
BRI3BP
140707
Affinity Capture-MS
Homo sapiens
30
LHX1
Affinity Capture-MS
Homo sapiens
31
HOOK1
Proximity Label-MS
Homo sapiens
32
SREBF2
6721
Affinity Capture-MS
Homo sapiens
33
RPA3
6119
Proximity Label-MS
Homo sapiens
34
GOLGB1
2804
Affinity Capture-MS
Homo sapiens
35
S1PR1
1901
Affinity Capture-MS
Homo sapiens
36
GPR45
Affinity Capture-MS
Homo sapiens
37
PLEKHD1
Cross-Linking-MS (XL-MS)
Homo sapiens
38
KIF14
9928
Affinity Capture-MS
Homo sapiens
39
CLTA
1211
Affinity Capture-MS
Homo sapiens
40
GPR182
Affinity Capture-MS
Homo sapiens
41
COPB2
9276
Affinity Capture-MS
Homo sapiens
42
CDK5RAP3
80279
Affinity Capture-MS
Homo sapiens
43
SMPD2
6610
Affinity Capture-MS
Homo sapiens
44
PRC1
9055
Affinity Capture-MS
Homo sapiens
45
TMED2
10959
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
46
CXCL9
Two-hybrid
Homo sapiens
47
DDRGK1
65992
Affinity Capture-MS
Homo sapiens
48
RNF13
11342
Affinity Capture-MS
Homo sapiens
49
FGFRL1
53834
Affinity Capture-MS
Homo sapiens
50
VIPR1
Affinity Capture-MS
Homo sapiens
51
ATP13A3
79572
Affinity Capture-MS
Homo sapiens
52
TMEM132D
Two-hybrid
Homo sapiens
53
ATP2A3
489
Affinity Capture-MS
Homo sapiens
54
PPP1R13B
Affinity Capture-MS
Homo sapiens
55
PRKRIR
Affinity Capture-MS
Homo sapiens
56
HERC5
51191
Affinity Capture-MS
Homo sapiens
57
GNL3L
54552
Cross-Linking-MS (XL-MS)
Homo sapiens
58
LIPG
9388
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which TMED5 is involved