ExoCarta: Gene summary

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Gene description for HERC5
Gene name	HECT and RLD domain containing E3 ubiquitin protein ligase 5
Gene symbol	HERC5
Other names/aliases	CEB1 CEBP1
Species	Homo sapiens
Database cross references - HERC5
ExoCarta	ExoCarta_51191
Vesiclepedia	VP_51191
Entrez Gene	51191
HGNC	24368
MIM	608242
UniProt	Q9UII4
HERC5 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells	33991177
Gastric cancer cells	33991177
Gastric cancer cells	33991177
Mesenchymal stem cells	Unpublished / Not applicable

Gene ontology annotations for HERC5

Molecular Function
	RNA binding	GO:0003723	HDA
	ubiquitin-protein transferase activity	GO:0004842	TAS
	protein binding	GO:0005515	IPI
	ISG15 transferase activity	GO:0042296	IBA
	ISG15 transferase activity	GO:0042296	IDA
	ubiquitin protein ligase activity	GO:0061630	IBA
	ubiquitin protein ligase activity	GO:0061630	IDA
Biological Process
	regulation of cyclin-dependent protein serine/threonine kinase activity	GO:0000079	TAS
	ubiquitin-dependent protein catabolic process	GO:0006511	IBA
	protein ubiquitination	GO:0016567	IBA
	protein ubiquitination	GO:0016567	IDA
	ISG15-protein conjugation	GO:0032020	IBA
	ISG15-protein conjugation	GO:0032020	IDA
	PKR/eIFalpha signaling	GO:0039585	TAS
	innate immune response	GO:0045087	IDA
	regulation of defense response to virus	GO:0050688	IDA
	defense response to virus	GO:0051607	IEA
Subcellular Localization
	cytoplasm	GO:0005737	IBA
	cytoplasm	GO:0005737	IDA
	cytoplasm	GO:0005737	IDA
	cytosol	GO:0005829	TAS
	perinuclear region of cytoplasm	GO:0048471	IEA

Experiment description of studies that identified HERC5 in sEVs

Experiment ID

363

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

364

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

365

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

126

MISEV standards
✘	Biophysical techniques
✔ GAPDH	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [LTQ-FT Ultra]

PubMed ID

Unpublished / Not applicable

Organism

Homo sapiens

Experiment description

Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?

Authors

"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"

Journal name

Mesenchymal Stem Cell Therapy

Publication year

2011

Sample

Mesenchymal stem cells

Sample name

huES9.E1

Isolation/purification methods

HPLC

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Antobody array
Mass spectrometry

Protein-protein interactions for HERC5

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

ISG15

9636

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

PITX2

Reconstituted Complex

Homo sapiens

ZNF346

Affinity Capture-MS

Homo sapiens

ANLN

54443

Affinity Capture-MS

Homo sapiens

ERCC2

2068

Reconstituted Complex

Homo sapiens

TMED5

50999

Affinity Capture-MS

Homo sapiens

RPL13A

23521

Affinity Capture-MS

Homo sapiens

RPL10

6134

Affinity Capture-MS

Homo sapiens

RPL15

6138

Affinity Capture-MS

Homo sapiens

RPL14

9045

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

USP36

Affinity Capture-MS

Homo sapiens

PARK2

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens

RPL10A

4736

Affinity Capture-MS

Homo sapiens

KIF23

9493

Affinity Capture-MS

Homo sapiens

FBXW7

Affinity Capture-MS

Homo sapiens

TMED2

10959

Affinity Capture-MS

Homo sapiens

NPM1

4869

Affinity Capture-MS

Homo sapiens

ANOS1

3730

Affinity Capture-MS

Homo sapiens

ZNF2

Affinity Capture-MS

Homo sapiens

SRP14

6727

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PRKRA

8575

Affinity Capture-MS

Homo sapiens

DNAJC7

7266

Proximity Label-MS

Homo sapiens

RRP8

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPS3A

6189

Affinity Capture-MS

Homo sapiens

NME2

4831

Affinity Capture-Western		Homo sapiens
Two-hybrid		Homo sapiens

NOP16

51491

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

OASL

8638

Affinity Capture-MS

Homo sapiens

RPL19

6143

Affinity Capture-MS

Homo sapiens

HSPA8

3312

Affinity Capture-Western

Homo sapiens

LIN28A

Affinity Capture-MS

Homo sapiens

CD70

970

Affinity Capture-MS

Homo sapiens

CDKN1A

Reconstituted Complex

Homo sapiens

RPL4

6124

Affinity Capture-MS

Homo sapiens

RPS10

6204

Affinity Capture-MS

Homo sapiens

RBM4B

Affinity Capture-MS

Homo sapiens

SRSF6

6431

Affinity Capture-MS

Homo sapiens

CCNB1

891

Reconstituted Complex

Homo sapiens

Srp72

Affinity Capture-MS

Mus musculus

RAN

5901

Affinity Capture-Western

Homo sapiens

NTRK1

4914

Affinity Capture-MS

Homo sapiens

IFI16

3428

Affinity Capture-MS

Homo sapiens

HIST1H1E

3008

Affinity Capture-MS

Homo sapiens

RPL36

25873

Affinity Capture-MS

Homo sapiens

GSG2

Affinity Capture-MS

Homo sapiens

HECTD1

25831

Affinity Capture-MS

Homo sapiens

RPLP0

6175

Affinity Capture-MS

Homo sapiens

MAGEB2

4113

Affinity Capture-MS

Homo sapiens

FGF13

Affinity Capture-MS

Homo sapiens

CCDC59

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FGFBP1

9982

Affinity Capture-MS

Homo sapiens

CCNA2

890

Reconstituted Complex

Homo sapiens

MAP7

9053

Affinity Capture-MS

Homo sapiens

ABT1

29777

Affinity Capture-MS

Homo sapiens

RPS2

6187

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RC3H2

Affinity Capture-MS

Homo sapiens

RBM47

54502

Affinity Capture-MS

Homo sapiens

SIRT7

Affinity Capture-MS

Homo sapiens

CTNNB1

1499

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

UBE2L6

9246

Reconstituted Complex

Homo sapiens

Nrip3

Affinity Capture-MS

Mus musculus

SURF6

Affinity Capture-MS

Homo sapiens

Rrbp1

Affinity Capture-MS

Mus musculus

CCNE1

Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens

TXNRD1

7296

Affinity Capture-Western

Homo sapiens

RPL3

6122

Affinity Capture-MS

Homo sapiens

FOXL1

Affinity Capture-MS

Homo sapiens

PDCD11

22984

Affinity Capture-MS

Homo sapiens

ZC3H10

Affinity Capture-MS

Homo sapiens

KIAA1467

57613

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NXF2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PRR3

Affinity Capture-MS

Homo sapiens

RPL18

6141

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DDRGK1

65992

Affinity Capture-MS

Homo sapiens

RANBP1

5902

Affinity Capture-Western

Homo sapiens

UBE2D2

7322

Reconstituted Complex

Homo sapiens

CCND1

595

Reconstituted Complex

Homo sapiens

ZC3H3

Affinity Capture-MS

Homo sapiens

IRF3

3661

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Biochemical Activity		Homo sapiens

RBMS2

5939

Affinity Capture-MS

Homo sapiens

UBE2D1

7321

Reconstituted Complex

Homo sapiens

RPL13

6137

Affinity Capture-MS

Homo sapiens

SNCA

6622

Affinity Capture-MS

Homo sapiens

TMED10

10972

Affinity Capture-MS

Homo sapiens

ILF3

3609

Affinity Capture-MS

Homo sapiens

FBXL6

Affinity Capture-MS

Homo sapiens

TP53

7157

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens

STK38

11329

Reconstituted Complex

Homo sapiens

SUV420H1

Affinity Capture-MS

Homo sapiens

DGCR8

Affinity Capture-MS

Homo sapiens

SMAD4

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

TTK

7272

Affinity Capture-MS

Homo sapiens

HIST1H1B

3009

Affinity Capture-MS

Homo sapiens

GPATCH4

54865

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

Mta1

Affinity Capture-MS

Mus musculus

UFL1

23376

Affinity Capture-MS

Homo sapiens

RBM4

5936

Affinity Capture-MS

Homo sapiens

NEIL1

Affinity Capture-MS

Homo sapiens

RPL23AP32

56969

Affinity Capture-MS

Homo sapiens

RPS6

6194

Affinity Capture-MS

Homo sapiens

100

FOXM1

Affinity Capture-MS

Homo sapiens

101

RPL23A

6147

Affinity Capture-MS

Homo sapiens

102

HNRNPU

3192

Affinity Capture-MS

Homo sapiens

103

GNB2L1

10399

Affinity Capture-MS

Homo sapiens

104

SRP9

6726

Affinity Capture-MS

Homo sapiens

105

RC3H1

149041

Affinity Capture-MS

Homo sapiens

106

HERC5

51191

Biochemical Activity		Homo sapiens
Biochemical Activity		Homo sapiens

View the network

Pathways in which HERC5 is involved

Pathway	Evidence	Source
Adaptive Immune System	TAS	Reactome
Antigen processing: Ubiquitination & Proteasome degradation	TAS	Reactome
Antiviral mechanism by IFN-stimulated genes	TAS	Reactome
Class I MHC mediated antigen processing & presentation	TAS	Reactome
Cytokine Signaling in Immune system	TAS	Reactome
DDX58/IFIH1-mediated induction of interferon-alpha/beta	TAS	Reactome
Disease	TAS	Reactome
Immune System	TAS	Reactome
Infectious disease	TAS	Reactome
Innate Immune System	TAS	Reactome
Interferon Signaling	TAS	Reactome
ISG15 antiviral mechanism	TAS	Reactome
Negative regulators of DDX58/IFIH1 signaling	TAS	Reactome
PKR-mediated signaling	TAS	Reactome
Respiratory Syncytial Virus Infection Pathway	TAS	Reactome
RSV-host interactions	TAS	Reactome
Viral Infection Pathways	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here