Gene description for STK38
Gene name serine/threonine kinase 38
Gene symbol STK38
Other names/aliases NDR
NDR1
Species Homo sapiens
 Database cross references - STK38
ExoCarta ExoCarta_11329
Vesiclepedia VP_11329
Entrez Gene 11329
HGNC 17847
MIM 606964
UniProt Q15208  
 STK38 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for STK38
Molecular Function
    magnesium ion binding GO:0000287 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IPI
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    cadherin binding GO:0045296 HDA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    ubiquitin-like protein reader activity GO:0140035 IDA
    histone reader activity GO:0140566 IDA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    UFM1-modified protein reader activity GO:0141185 IDA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    DNA damage checkpoint signaling GO:0000077 IDA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    DNA damage response GO:0006974 IDA
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IDA
    protein modification process GO:0036211 IDA
    negative regulation of MAP kinase activity GO:0043407 IDA
    postsynapse organization GO:0099173 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    site of double-strand break GO:0035861 IDA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified STK38 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STK38
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRBN  
Affinity Capture-MS Homo sapiens
2 Mdm2  
Affinity Capture-MS Mus musculus
3 HIST2H2BE 8349
Biochemical Activity Homo sapiens
4 Chek2  
Affinity Capture-MS Mus musculus
5 UBE2H 7328
Affinity Capture-MS Homo sapiens
6 ISG15 9636
Affinity Capture-MS Homo sapiens
7 GJA1 2697
Affinity Capture-MS Homo sapiens
8 SKI 6497
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
9 RPA2 6118
Proximity Label-MS Homo sapiens
10 GTF2F1 2962
Co-fractionation Homo sapiens
11 HRAS 3265
Reconstituted Complex Homo sapiens
12 CALM1 801
Reconstituted Complex Homo sapiens
13 ZBTB1  
Affinity Capture-MS Homo sapiens
14 KIAA1429 25962
Affinity Capture-MS Homo sapiens
15 D2HGDH 728294
Affinity Capture-MS Homo sapiens
16 EIF4A3 9775
Affinity Capture-MS Homo sapiens
17 PLK4  
Affinity Capture-MS Homo sapiens
18 FBXW7  
Affinity Capture-Western Homo sapiens
19 AGO3  
Affinity Capture-MS Homo sapiens
20 Stk38 106504
Affinity Capture-MS Mus musculus
21 PRDM4  
Affinity Capture-MS Homo sapiens
22 DNAJB1 3337
Co-fractionation Homo sapiens
23 HIST2H2AC 8338
Biochemical Activity Homo sapiens
24 MPP1 4354
Co-fractionation Homo sapiens
25 MECP2 4204
Affinity Capture-MS Homo sapiens
26 SNX27 81609
Affinity Capture-MS Homo sapiens
27 VDR  
Affinity Capture-MS Homo sapiens
28 LIN28A  
Affinity Capture-MS Homo sapiens
29 YWHAB 7529
Affinity Capture-MS Homo sapiens
30 BANP  
Two-hybrid Homo sapiens
31 MAP3K2 10746
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 PRPH 5630
Proximity Label-MS Homo sapiens
33 CTDP1 9150
Affinity Capture-Western Homo sapiens
34 SHMT1 6470
Co-fractionation Homo sapiens
35 PSMD14 10213
Affinity Capture-MS Homo sapiens
36 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
37 CCNB1 891
Affinity Capture-MS Homo sapiens
38 CDH1 999
Proximity Label-MS Homo sapiens
39 IFI16 3428
Affinity Capture-MS Homo sapiens
40 AGR2 10551
Co-fractionation Homo sapiens
41 S100B 6285
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
42 MAGOH 4116
Affinity Capture-MS Homo sapiens
43 CYLD  
Affinity Capture-MS Homo sapiens
44 ID2  
Affinity Capture-MS Homo sapiens
45 FBXL4 26235
Affinity Capture-MS Homo sapiens
46 PHLPP1  
Proximity Label-MS Homo sapiens
47 GAN 8139
Affinity Capture-MS Homo sapiens
48 MARVELD2 153562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 SOCS2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
50 MOB1B 92597
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
51 YWHAZ 7534
Affinity Capture-MS Homo sapiens
52 RNF43  
Proximity Label-MS Homo sapiens
53 PRKCZ 5590
Affinity Capture-MS Homo sapiens
54 CDK19 23097
Affinity Capture-Western Homo sapiens
55 RICTOR 253260
Affinity Capture-Western Homo sapiens
56 MAP3K1 4214
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
57 FBXO6 26270
Affinity Capture-MS Homo sapiens
58 DUSP16  
Affinity Capture-MS Homo sapiens
59 VHL  
Affinity Capture-MS Homo sapiens
60 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
61 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 MTOR 2475
Affinity Capture-Western Homo sapiens
64 MOB2 81532
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
65 MYSM1  
Affinity Capture-MS Homo sapiens
66 GRK5 2869
Affinity Capture-MS Homo sapiens
67 MOB1A 55233
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
68 ARRB2 409
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
69 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
70 MAPKAP1 79109
Affinity Capture-Western Homo sapiens
71 TP53 7157
Affinity Capture-MS Homo sapiens
72 GBP1 2633
Co-fractionation Homo sapiens
73 CDK8 1024
Affinity Capture-Western Homo sapiens
74 STK38 11329
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
75 SMURF1 57154
Affinity Capture-MS Homo sapiens
76 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
77 STK38L 23012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 MTHFD1 4522
Affinity Capture-MS Homo sapiens
79 TRIM25 7706
Reconstituted Complex Homo sapiens
80 ESR1  
Affinity Capture-MS Homo sapiens
81 UFL1 23376
Affinity Capture-MS Homo sapiens
82 BECN1 8678
Affinity Capture-Western Homo sapiens
83 ARRB1 408
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
84 TCF3  
Affinity Capture-MS Homo sapiens
85 TXNIP 10628
Affinity Capture-MS Homo sapiens
86 CCDC96  
Affinity Capture-MS Homo sapiens
87 RC3H1 149041
Affinity Capture-MS Homo sapiens
88 PRMT1 3276
Affinity Capture-MS Homo sapiens
89 NOTCH1 4851
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
90 HERC5 51191
Reconstituted Complex Homo sapiens
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