Gene description for GRK5
Gene name G protein-coupled receptor kinase 5
Gene symbol GRK5
Other names/aliases GPRK5
Species Homo sapiens
 Database cross references - GRK5
ExoCarta ExoCarta_2869
Vesiclepedia VP_2869
Entrez Gene 2869
HGNC 4544
MIM 600870
UniProt P34947  
 GRK5 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for GRK5
Molecular Function
    protein kinase activity GO:0004672 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    G protein-coupled receptor kinase activity GO:0004703 IEA
    protein kinase C binding GO:0005080 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    phospholipid binding GO:0005543 TAS
    beta-adrenergic receptor kinase activity GO:0047696 IMP
    beta-adrenergic receptor kinase activity GO:0047696 TAS
Biological Process
    apoptotic process GO:0006915 IEA
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 TAS
    tachykinin receptor signaling pathway GO:0007217 IDA
    regulation of G protein-coupled receptor signaling pathway GO:0008277 TAS
    positive regulation of cell population proliferation GO:0008284 IMP
    regulation of signal transduction GO:0009966 IBA
    Wnt signaling pathway GO:0016055 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    fat cell differentiation GO:0045444 IEA
    protein autophosphorylation GO:0046777 IDA
    regulation of cell cycle GO:0051726 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    nuclear speck GO:0016607 IDA
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified GRK5 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GRK5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Affinity Capture-MS Homo sapiens
2 MRPS27 23107
Affinity Capture-MS Homo sapiens
3 SNCB 6620
Biochemical Activity Homo sapiens
4 NCBP1 4686
Affinity Capture-MS Homo sapiens
5 LYZ 4069
Affinity Capture-MS Homo sapiens
6 NOLC1 9221
Affinity Capture-MS Homo sapiens
7 RCVRN  
Affinity Capture-Western Homo sapiens
8 DOCK4 9732
Affinity Capture-MS Homo sapiens
9 NFIB  
Affinity Capture-MS Homo sapiens
10 WDR82 80335
Affinity Capture-MS Homo sapiens
11 EIF2S3 1968
Affinity Capture-MS Homo sapiens
12 PSMD11 5717
Affinity Capture-MS Homo sapiens
13 KPNB1 3837
Affinity Capture-MS Homo sapiens
14 CAND2 23066
Affinity Capture-MS Homo sapiens
15 MAP4 4134
Affinity Capture-MS Homo sapiens
16 USP9X 8239
Affinity Capture-MS Homo sapiens
17 TAB1 10454
Affinity Capture-MS Homo sapiens
18 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
19 CALM1 801
Reconstituted Complex Homo sapiens
20 CFL1 1072
Affinity Capture-MS Homo sapiens
21 PRKDC 5591
Affinity Capture-MS Homo sapiens
22 LRPPRC 10128
Affinity Capture-MS Homo sapiens
23 DAXX  
Affinity Capture-MS Homo sapiens
24 TBL2 26608
Affinity Capture-MS Homo sapiens
25 RUVBL1 8607
Affinity Capture-MS Homo sapiens
26 GLG1 2734
Affinity Capture-MS Homo sapiens
27 AFF4 27125
Affinity Capture-MS Homo sapiens
28 RHO  
Biochemical Activity Homo sapiens
29 BAZ1A 11177
Affinity Capture-MS Homo sapiens
30 SPIN1  
Affinity Capture-MS Homo sapiens
31 AP2A1 160
Affinity Capture-MS Homo sapiens
32 SMARCA1 6594
Affinity Capture-MS Homo sapiens
33 KCTD5 54442
Affinity Capture-MS Homo sapiens
34 SRRM2 23524
Affinity Capture-MS Homo sapiens
35 WDR12 55759
Affinity Capture-MS Homo sapiens
36 YWHAG 7532
Affinity Capture-MS Homo sapiens
37 FMNL2 114793
Affinity Capture-MS Homo sapiens
38 AGTR1  
Affinity Capture-Western Homo sapiens
39 GRWD1 83743
Affinity Capture-MS Homo sapiens
40 GINS4  
Affinity Capture-MS Homo sapiens
41 ATG16L1 55054
Affinity Capture-MS Homo sapiens
42 CALR 811
Affinity Capture-MS Homo sapiens
43 PNPLA6 10908
Affinity Capture-MS Homo sapiens
44 PPP6R3 55291
Affinity Capture-MS Homo sapiens
45 SPIN3  
Affinity Capture-MS Homo sapiens
46 MYCN  
Affinity Capture-MS Homo sapiens
47 FAM120A 23196
Affinity Capture-MS Homo sapiens
48 YWHAB 7529
Affinity Capture-MS Homo sapiens
49 TUFM 7284
Affinity Capture-MS Homo sapiens
50 TCOF1 6949
Affinity Capture-MS Homo sapiens
51 PSMC2 5701
Affinity Capture-MS Homo sapiens
52 PIP 5304
Affinity Capture-MS Homo sapiens
53 TOR4A 54863
Affinity Capture-MS Homo sapiens
54 SNCA  
Biochemical Activity Bos taurus
55 DDX21 9188
Affinity Capture-MS Homo sapiens
56 RBX1 9978
Affinity Capture-Western Homo sapiens
57 CTDP1 9150
Affinity Capture-MS Homo sapiens
58 CUL4B 8450
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
59 RPL4 6124
Affinity Capture-MS Homo sapiens
60 PSMC1 5700
Affinity Capture-MS Homo sapiens
61 ACIN1 22985
Affinity Capture-MS Homo sapiens
62 SPATA31A7  
Affinity Capture-MS Homo sapiens
63 PSMD2 5708
Affinity Capture-MS Homo sapiens
64 MYH9 4627
Affinity Capture-MS Homo sapiens
65 CKAP5 9793
Affinity Capture-MS Homo sapiens
66 GYS1 2997
Affinity Capture-MS Homo sapiens
67 CABP1 9478
Reconstituted Complex Homo sapiens
68 MMS19 64210
Affinity Capture-MS Homo sapiens
69 YWHAQ 10971
Affinity Capture-MS Homo sapiens
70 UBTF 7343
Affinity Capture-MS Homo sapiens
71 LYAR 55646
Affinity Capture-MS Homo sapiens
72 MYH10 4628
Affinity Capture-MS Homo sapiens
73 DHX9 1660
Affinity Capture-MS Homo sapiens
74 ABCF1 23
Affinity Capture-MS Homo sapiens
75 AP3B1 8546
Affinity Capture-MS Homo sapiens
76 RAB11FIP5 26056
Affinity Capture-MS Homo sapiens
77 NCL 4691
Affinity Capture-MS Homo sapiens
78 AVPR2  
Affinity Capture-Western Homo sapiens
79 SRPK1 6732
Affinity Capture-MS Homo sapiens
80 LARP1 23367
Affinity Capture-MS Homo sapiens
81 MAT2A 4144
Affinity Capture-MS Homo sapiens
82 BCLAF1 9774
Affinity Capture-MS Homo sapiens
83 OLA1 29789
Affinity Capture-MS Homo sapiens
84 BMS1  
Affinity Capture-MS Homo sapiens
85 TNFAIP8L1  
Affinity Capture-MS Homo sapiens
86 COPS2 9318
Affinity Capture-MS Homo sapiens
87 HADHB 3032
Affinity Capture-MS Homo sapiens
88 COPS7A 50813
Affinity Capture-MS Homo sapiens
89 MRTO4 51154
Affinity Capture-MS Homo sapiens
90 USP15 9958
Affinity Capture-MS Homo sapiens
91 PNN 5411
Affinity Capture-MS Homo sapiens
92 PSMC6 5706
Affinity Capture-MS Homo sapiens
93 NAT10 55226
Affinity Capture-MS Homo sapiens
94 EEF2 1938
Affinity Capture-MS Homo sapiens
95 SRSF1 6426
Affinity Capture-MS Homo sapiens
96 KCMF1 56888
Affinity Capture-MS Homo sapiens
97 MAP4K4 9448
Affinity Capture-MS Homo sapiens
98 MADD 8567
Affinity Capture-MS Homo sapiens
99 YWHAZ 7534
Affinity Capture-MS Homo sapiens
100 DDB2  
Affinity Capture-Western Homo sapiens
101 RPL3 6122
Affinity Capture-MS Homo sapiens
102 RBMS1 5937
Affinity Capture-MS Homo sapiens
103 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
104 ABI3BP 25890
Affinity Capture-MS Homo sapiens
105 DHX30 22907
Affinity Capture-MS Homo sapiens
106 NAF1  
Affinity Capture-MS Homo sapiens
107 EEF1A1 1915
Affinity Capture-MS Homo sapiens
108 MYO1C 4641
Affinity Capture-MS Homo sapiens
109 NXF1 10482
Affinity Capture-RNA Homo sapiens
110 AVPR1A 552
Affinity Capture-Western Homo sapiens
111 EFTUD2 9343
Affinity Capture-MS Homo sapiens
112 RANBP9 10048
Affinity Capture-MS Homo sapiens
113 GEMIN4 50628
Affinity Capture-MS Homo sapiens
114 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
115 ANKFY1 51479
Affinity Capture-MS Homo sapiens
116 USP47 55031
Affinity Capture-MS Homo sapiens
117 GOT1L1  
Affinity Capture-MS Homo sapiens
118 DLST 1743
Affinity Capture-MS Homo sapiens
119 AP2A2 161
Affinity Capture-MS Homo sapiens
120 MYO9B 4650
Affinity Capture-MS Homo sapiens
121 AKT2 208
Affinity Capture-MS Homo sapiens
122 HSPA4 3308
Affinity Capture-MS Homo sapiens
123 PPFIBP1 8496
Affinity Capture-MS Homo sapiens
124 SKIV2L2 23517
Affinity Capture-MS Homo sapiens
125 DDB1 1642
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
126 NR2F1  
Affinity Capture-MS Homo sapiens
127 FTSJ3 117246
Affinity Capture-MS Homo sapiens
128 XRCC5 7520
Affinity Capture-MS Homo sapiens
129 SPTBN1 6711
Affinity Capture-MS Homo sapiens
130 U2SURP 23350
Affinity Capture-MS Homo sapiens
131 TBXA2R 6915
Biochemical Activity Homo sapiens
132 DHX15 1665
Affinity Capture-MS Homo sapiens
133 GRK5 2869
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
134 SNCA 6622
Biochemical Activity Homo sapiens
135 SPTAN1 6709
Affinity Capture-MS Homo sapiens
136 CLIC1 1192
Affinity Capture-MS Homo sapiens
137 TACR1  
Biochemical Activity Homo sapiens
138 GRK6 2870
Affinity Capture-MS Homo sapiens
139 ILF3 3609
Affinity Capture-MS Homo sapiens
140 JUP 3728
Affinity Capture-MS Homo sapiens
141 SRRM1 10250
Affinity Capture-MS Homo sapiens
142 UBE2O 63893
Affinity Capture-MS Homo sapiens
143 SIN3A  
Affinity Capture-MS Homo sapiens
144 FRYL 285527
Affinity Capture-MS Homo sapiens
145 STK38 11329
Affinity Capture-MS Homo sapiens
146 PPIG 9360
Affinity Capture-MS Homo sapiens
147 HDAC6 10013
Affinity Capture-MS Homo sapiens
148 KANK1  
Affinity Capture-MS Homo sapiens
149 SNCG 6623
Biochemical Activity Homo sapiens
150 CDC37 11140
Affinity Capture-MS Homo sapiens
151 SKP1 6500
Affinity Capture-MS Homo sapiens
152 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
153 EIF4A3 9775
Affinity Capture-MS Homo sapiens
154 ARF4 378
Affinity Capture-MS Homo sapiens
155 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
156 OTUD4  
Affinity Capture-MS Homo sapiens
157 MOV10 4343
Affinity Capture-RNA Homo sapiens
158 ALYREF 10189
Affinity Capture-MS Homo sapiens
159 KANK2 25959
Affinity Capture-MS Homo sapiens
160 ARF1 375
Affinity Capture-MS Homo sapiens
161 C1QBP 708
Affinity Capture-MS Homo sapiens
162 AP3M1 26985
Affinity Capture-MS Homo sapiens
163 HNRNPU 3192
Affinity Capture-MS Homo sapiens
164 CWC22 57703
Affinity Capture-MS Homo sapiens
165 COPS4 51138
Affinity Capture-MS Homo sapiens
166 AP3D1 8943
Affinity Capture-MS Homo sapiens
167 SAP18 10284
Affinity Capture-MS Homo sapiens
168 AP3M2  
Affinity Capture-MS Homo sapiens
169 CAV1 857
Reconstituted Complex Homo sapiens
170 DCAF5  
Affinity Capture-MS Homo sapiens
171 CUL4A 8451
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
172 MAEA 10296
Affinity Capture-MS Homo sapiens
173 BCR 613
Affinity Capture-MS Homo sapiens
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