Gene description for GRK5
Gene name G protein-coupled receptor kinase 5
Gene symbol GRK5
Other names/aliases GPRK5
Species Homo sapiens
 Database cross references - GRK5
ExoCarta ExoCarta_2869
Vesiclepedia VP_2869
Entrez Gene 2869
HGNC 4544
MIM 600870
UniProt P34947  
 GRK5 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for GRK5
Molecular Function
    beta-adrenergic receptor kinase activity GO:0047696 IMP
    protein kinase C binding GO:0005080 TAS
    ATP binding GO:0005524 IEA
    G-protein coupled receptor kinase activity GO:0004703 IEA
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 TAS
    protein serine/threonine kinase activity GO:0004674 IDA
Biological Process
    protein autophosphorylation GO:0046777 IDA
    termination of G-protein coupled receptor signaling pathway GO:0038032 IEA
    tachykinin receptor signaling pathway GO:0007217 IDA
    regulation of cell cycle GO:0051726 IMP
    positive regulation of cell proliferation GO:0008284 IMP
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 TAS
    regulation of G-protein coupled receptor protein signaling pathway GO:0008277 TAS
    Wnt signaling pathway GO:0016055 IEA
    apoptotic process GO:0006915 IEA
    negative regulation of apoptotic process GO:0043066 IDA
Subcellular Localization
    nuclear membrane GO:0031965 IDA
    focal adhesion GO:0005925 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified GRK5 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GRK5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AVPR1A 552
Affinity Capture-Western Homo sapiens
2 OXTR 5021
Affinity Capture-Western Homo sapiens
3 SNCG 6623
Invitro Homo sapiens
4 CHRM2  
Invitro Homo sapiens
5 TACR1  
Invitro Homo sapiens
6 GIT1 28964
Invivo Homo sapiens
7 AGTR1  
Affinity Capture-Western Homo sapiens
8 CALM1 801
Reconstituted Complex Homo sapiens
9 RCV1  
Invivo Homo sapiens
10 RHO  
Invitro Homo sapiens
Invitro Homo sapiens
11 CAV1 857
Reconstituted Complex Homo sapiens
12 AVPR2  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which GRK5 is involved
PathwayEvidenceSource
G alpha (q) signalling events TAS Reactome
G alpha (q) signalling events TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here