Gene description for MOB1B
Gene name MOB kinase activator 1B
Gene symbol MOB1B
Other names/aliases MATS2
MOB4A
MOBKL1A
Species Homo sapiens
 Database cross references - MOB1B
ExoCarta ExoCarta_92597
Vesiclepedia VP_92597
Entrez Gene 92597
HGNC 29801
MIM 609282
UniProt Q7L9L4  
 MOB1B identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
Urine 15326289    
 Gene ontology annotations for MOB1B
Molecular Function
    protein binding GO:0005515 IPI
    kinase activator activity GO:0019209 IDA
    kinase binding GO:0019900 IPI
    protein kinase activator activity GO:0030295 IBA
    protein serine/threonine kinase activator activity GO:0043539 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    regulation of protein autophosphorylation GO:0031952 IDA
    hippo signaling GO:0035329 IBA
    hippo signaling GO:0035329 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MOB1B in exosomes
1
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for MOB1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DST 667
Proximity Label-MS Homo sapiens
2 STK4 6789
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
3 STK3 6788
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
4 TNIP1 10318
Two-hybrid Homo sapiens
5 RASSF2 9770
Affinity Capture-MS Homo sapiens
6 KLC2 64837
Affinity Capture-MS Homo sapiens
7 SOD1 6647
Affinity Capture-MS Homo sapiens
8 DOCK8 81704
Proximity Label-MS Homo sapiens
9 SKI 6497
Affinity Capture-Western Homo sapiens
10 DOCK7 85440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 STK38 11329
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RPA2 6118
Proximity Label-MS Homo sapiens
13 CAV2 858
Affinity Capture-MS Homo sapiens
14 IPO11 51194
Proximity Label-MS Homo sapiens
15 AJUBA  
Affinity Capture-MS Homo sapiens
16 MOB1A 55233
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 MAP4K4 9448
Proximity Label-MS Homo sapiens
18 SLK 9748
Affinity Capture-MS Homo sapiens
19 HSD17B10 3028
Affinity Capture-MS Homo sapiens
20 PPP6R2 9701
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
21 STK38L 23012
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PPP6R3 55291
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
23 ANKRD28 23243
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
24 DOCK6 57572
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
25 APP 351
Reconstituted Complex Homo sapiens
26 LATS1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PKN2 5586
Affinity Capture-MS Homo sapiens
28 NT5C2 22978
Two-hybrid Homo sapiens
29 ANKRD52 283373
Proximity Label-MS Homo sapiens
30 RPA3 6119
Proximity Label-MS Homo sapiens
31 WIBG 84305
Affinity Capture-MS Homo sapiens
32 LRCH1 23143
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
33 RASSF5 83593
Proximity Label-MS Homo sapiens
34 SAV1  
Affinity Capture-MS Homo sapiens
35 PPP6C 5537
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
36 TLN1 7094
Cross-Linking-MS (XL-MS) Homo sapiens
37 MOB2 81532
Proximity Label-MS Homo sapiens
38 CCDC53 51019
Two-hybrid Homo sapiens
39 PPP6R1 22870
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
40 LRCH2  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 PPIF 10105
Co-fractionation Homo sapiens
42 LATS2 26524
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 CRLF3 51379
Affinity Capture-MS Homo sapiens
44 ZNF787  
Affinity Capture-MS Homo sapiens
45 CYLD  
Affinity Capture-MS Homo sapiens
46 Stk38 106504
Affinity Capture-MS Mus musculus
47 LRCH3 84859
Proximity Label-MS Homo sapiens
48 PTPN14 5784
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MOB1B is involved
PathwayEvidenceSource
Signal Transduction TAS Reactome
Signaling by Hippo TAS Reactome





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