Gene description for CCDC53
Gene name coiled-coil domain containing 53
Gene symbol CCDC53
Other names/aliases CGI-116
Species Homo sapiens
 Database cross references - CCDC53
ExoCarta ExoCarta_51019
Vesiclepedia VP_51019
Entrez Gene 51019
HGNC 24256
UniProt Q9Y3C0  
 CCDC53 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CCDC53
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    exocytosis GO:0006887 IBA
    protein transport GO:0015031 IEA
    endosomal transport GO:0016197 NAS
    actin filament polymerization GO:0030041 IBA
    regulation of Arp2/3 complex-mediated actin nucleation GO:0034315 NAS
Subcellular Localization
    early endosome membrane GO:0031901 NAS
    WASH complex GO:0071203 IBA
    WASH complex GO:0071203 IDA
    WASH complex GO:0071203 NAS
 Experiment description of studies that identified CCDC53 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CCDC53
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCRS1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 VPS35 55737
Affinity Capture-MS Homo sapiens
3 KRT27 342574
Two-hybrid Homo sapiens
4 BLOC1S6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 KIAA0196 9897
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SYCE1  
Two-hybrid Homo sapiens
7 HSBP1 3281
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 WASF2 10163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ACAA1 30
Affinity Capture-MS Homo sapiens
10 KRT6A 3853
Two-hybrid Homo sapiens
11 CEP55 55165
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 NUP62 23636
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 GDF9 2661
Two-hybrid Homo sapiens
14 MGAT4A 11320
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 FAM21A 387680
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 BFSP2  
Two-hybrid Homo sapiens
17 SETDB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LINC00521  
Two-hybrid Homo sapiens
19 VPS29 51699
Affinity Capture-MS Homo sapiens
20 SNAPIN 23557
Two-hybrid Homo sapiens
21 HSF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ATG13 9776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 LAMC1 3915
Two-hybrid Homo sapiens
24 BLOC1S1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 KRT71 112802
Two-hybrid Homo sapiens
26 WASH3P  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 NDRG1 10397
Affinity Capture-MS Homo sapiens
28 NAV1 89796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 WASH6P  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MCTS1 28985
Affinity Capture-MS Homo sapiens
31 POC1B 282809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CD276 80381
Affinity Capture-MS Homo sapiens
33 WASH1 100287171
Two-hybrid Homo sapiens
34 GGA1 26088
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 IVD 3712
Affinity Capture-MS Homo sapiens
36 GOLGA4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 LHX4  
Two-hybrid Homo sapiens
38 SNX27 81609
Affinity Capture-MS Homo sapiens
39 KNSTRN  
Two-hybrid Homo sapiens
40 SDCBP 6386
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
41 WASF3 10810
Affinity Capture-MS Homo sapiens
42 IFT20 90410
Two-hybrid Homo sapiens
43 SRGAP2B  
Two-hybrid Homo sapiens
44 C15orf59  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
45 CENPK  
Two-hybrid Homo sapiens
46 BANP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SRP9 6726
Affinity Capture-MS Homo sapiens
48 ZFP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 IKBIP 121457
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
50 CAPZA1 829
Affinity Capture-MS Homo sapiens
51 WASF1 8936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 TEX35  
Two-hybrid Homo sapiens
53 RB1CC1 9821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ALDH1A2 8854
Affinity Capture-MS Homo sapiens
55 OPTN 10133
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
56 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
57 POC1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 DTNBP1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
59 GCC2 9648
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 PBXIP1 57326
Two-hybrid Homo sapiens
61 PPP1R8 5511
Affinity Capture-MS Homo sapiens
62 KRT75 9119
Two-hybrid Homo sapiens
63 RAP1A 5906
Affinity Capture-MS Homo sapiens
64 FKBP15 23307
Affinity Capture-MS Homo sapiens
65 LUC7L2 51631
Two-hybrid Homo sapiens
66 BICD2 23299
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 ULK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 HJURP  
Affinity Capture-MS Homo sapiens
69 FAM49B 51571
Affinity Capture-MS Homo sapiens
70 KIAA1033 23325
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 ACTG1 71
Affinity Capture-MS Homo sapiens
72 SNX1 6642
Affinity Capture-MS Homo sapiens
73 PTN  
Two-hybrid Homo sapiens
74 RNF40 9810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 PER2  
Affinity Capture-MS Homo sapiens
76 GOLGA3 2802
Affinity Capture-MS Homo sapiens
77 MOB1B 92597
Two-hybrid Homo sapiens
78 LMNB2 84823
Two-hybrid Homo sapiens
79 TXLNB  
Two-hybrid Homo sapiens
80 TRAF6 7189
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 MYOZ1  
Two-hybrid Homo sapiens
82 RABGAP1 23637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 Wash  
Affinity Capture-MS Mus musculus
84 GNG13  
Two-hybrid Homo sapiens
85 COG6 57511
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
86 ZMPSTE24 10269
Affinity Capture-MS Homo sapiens
87 SCARA5 286133
Two-hybrid Homo sapiens
88 FAM21C 253725
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 NDC80 10403
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
90 HDLBP 3069
Affinity Capture-MS Homo sapiens
91 PCM1 5108
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
92 TKFC 26007
Affinity Capture-MS Homo sapiens
93 NAA10 8260
Affinity Capture-MS Homo sapiens
94 DNAJC13 23317
Affinity Capture-MS Homo sapiens
95 TXLNA 200081
Two-hybrid Homo sapiens
96 HAUS1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
97 IL18 3606
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 PAPOLA 10914
Affinity Capture-MS Homo sapiens
99 BLOC1S2 282991
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
100 HORMAD2  
Affinity Capture-MS Homo sapiens
101 CCHCR1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
102 VPS52 6293
Two-hybrid Homo sapiens
103 IER3IP1 51124
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 ARL8A 127829
Affinity Capture-MS Homo sapiens
105 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 EPHX1 2052
Affinity Capture-MS Homo sapiens
107 ATG101  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 HSPB1 3315
Two-hybrid Homo sapiens
109 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
110 RNF20 56254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 TBC1D2B 23102
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 PHOSPHO2  
Two-hybrid Homo sapiens
113 BRK1 55845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 C20orf202  
Two-hybrid Homo sapiens
115 ABI2 10152
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
116 KDM1A 23028
Two-hybrid Homo sapiens
117 BLOC1S4 55330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 RAB7A 7879
Proximity Label-MS Homo sapiens
119 SNX2 6643
Affinity Capture-MS Homo sapiens
120 MOB1A 55233
Two-hybrid Homo sapiens
121 CKAP4 10970
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CCDC53 is involved
No pathways found





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