Gene description for PPP1R8
Gene name protein phosphatase 1, regulatory subunit 8
Gene symbol PPP1R8
Other names/aliases ARD-1
ARD1
NIPP-1
NIPP1
PRO2047
Species Homo sapiens
 Database cross references - PPP1R8
ExoCarta ExoCarta_5511
Vesiclepedia VP_5511
Entrez Gene 5511
HGNC 9296
MIM 602636
UniProt Q12972  
 PPP1R8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PPP1R8
Molecular Function
    DNA binding GO:0003677 IEA
    RNA binding GO:0003723 TAS
    mRNA binding GO:0003729 IBA
    endonuclease activity GO:0004519 IEA
    protein serine/threonine phosphatase inhibitor activity GO:0004865 IBA
    protein binding GO:0005515 IPI
    ribonuclease E activity GO:0008995 TAS
    molecular function inhibitor activity GO:0140678 EXP
Biological Process
    mRNA processing GO:0006397 IEA
    RNA catabolic process GO:0006401 TAS
    cell population proliferation GO:0008283 IEA
    RNA splicing GO:0008380 IEA
Subcellular Localization
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 IEA
    cytoplasm GO:0005737 IEA
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified PPP1R8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPP1R8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RBM48  
Two-hybrid Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 RIPK2  
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 NME2 4831
Affinity Capture-MS Homo sapiens
6 WIF1 11197
Affinity Capture-MS Homo sapiens
7 MRPL42  
Affinity Capture-MS Homo sapiens
8 PPP1CB 5500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
9 PPP1R3A  
Affinity Capture-MS Homo sapiens
10 HDAC2 3066
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 SARS 6301
Co-fractionation Homo sapiens
12 PRPF4B 8899
Affinity Capture-MS Homo sapiens
13 PREP 5550
Co-fractionation Homo sapiens
14 WDR77 79084
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 CPSF6 11052
Affinity Capture-MS Homo sapiens
16 C14orf1 11161
Two-hybrid Homo sapiens
17 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SNUPN 10073
Co-fractionation Homo sapiens
19 SF3A1 10291
Affinity Capture-MS Homo sapiens
20 SNRPB 6628
Affinity Capture-MS Homo sapiens
21 STXBP1 6812
Co-fractionation Homo sapiens
22 NF2 4771
Co-fractionation Homo sapiens
23 RAD51D  
Affinity Capture-MS Homo sapiens
24 PPP1CA 5499
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
25 Ppp1cb 19046
Affinity Capture-MS Mus musculus
26 FAM98B 283742
Co-fractionation Homo sapiens
27 CTNNBL1 56259
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 SNRPA 6626
Affinity Capture-MS Homo sapiens
29 HDDC3  
Affinity Capture-MS Homo sapiens
30 TOP1 7150
Affinity Capture-MS Homo sapiens
31 RBM39 9584
Affinity Capture-MS Homo sapiens
32 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 U2AF2 11338
Affinity Capture-MS Homo sapiens
34 PPIL1 51645
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
35 CNKSR1  
Reconstituted Complex Homo sapiens
36 GBP2  
Two-hybrid Homo sapiens
37 SNRPF 6636
Affinity Capture-MS Homo sapiens
38 SETDB1  
Two-hybrid Homo sapiens
39 SUZ12  
Affinity Capture-Western Homo sapiens
40 APP 351
Reconstituted Complex Homo sapiens
41 LYN 4067
Biochemical Activity Homo sapiens
42 SHMT2 6472
Co-fractionation Homo sapiens
43 DNAJB6 10049
Affinity Capture-MS Homo sapiens
44 CCDC53 51019
Affinity Capture-MS Homo sapiens
45 PPP1CC 5501
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
46 HOXC5  
Affinity Capture-MS Homo sapiens
47 PRKACA 5566
Biochemical Activity Homo sapiens
48 Cep72  
Affinity Capture-MS Mus musculus
49 EZH2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
50 USP47 55031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 LRRK2 120892
Reconstituted Complex Homo sapiens
52 GTF2E2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 RTCB 51493
Co-fractionation Homo sapiens
54 EZR 7430
Co-fractionation Homo sapiens
55 RTN4IP1  
Two-hybrid Homo sapiens
56 SF3B1 23451
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
57 RPL35A 6165
Affinity Capture-MS Homo sapiens
58 BICD2 23299
Proximity Label-MS Homo sapiens
59 FBXO25  
Biochemical Activity Homo sapiens
60 KRAS 3845
Negative Genetic Homo sapiens
61 DDX1 1653
Co-fractionation Homo sapiens
62 SNRNP27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 TGM1 7051
Affinity Capture-MS Homo sapiens
64 STC2 8614
Two-hybrid Homo sapiens
65 APPBP2  
Two-hybrid Homo sapiens
66 CRMP1 1400
Two-hybrid Homo sapiens
67 SSRP1 6749
Affinity Capture-MS Homo sapiens
68 SF3A2 8175
Affinity Capture-MS Homo sapiens
69 DDX39B 7919
Affinity Capture-MS Homo sapiens
70 VPS4B 9525
Co-fractionation Homo sapiens
71 SNRPC 6631
Affinity Capture-MS Homo sapiens
72 RBBP4 5928
Affinity Capture-Western Homo sapiens
73 EED  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
74 SHC1 6464
Co-fractionation Homo sapiens
View the network image/svg+xml



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