Gene description for U2AF2
Gene name U2 small nuclear RNA auxiliary factor 2
Gene symbol U2AF2
Other names/aliases U2AF65
Species Homo sapiens
 Database cross references - U2AF2
ExoCarta ExoCarta_11338
Entrez Gene 11338
HGNC 23156
MIM 191318
UniProt P26368  
 U2AF2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
 Gene ontology annotations for U2AF2
Molecular Function
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    C2H2 zinc finger domain binding GO:0070742 IEA
    poly(A) RNA binding GO:0044822 IDA
    enzyme binding GO:0019899 IPI
Biological Process
    gene expression GO:0010467 TAS
    mRNA export from nucleus GO:0006406 TAS
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    positive regulation of protein targeting to mitochondrion GO:1903955 IMP
    RNA splicing GO:0008380 TAS
    positive regulation of RNA splicing GO:0033120 IDA
    mRNA splicing, via spliceosome GO:0000398 TAS
    mRNA processing GO:0006397 TAS
    regulation of mitochondrion degradation GO:1903146 IMP
    mRNA 3'-end processing GO:0031124 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nuclear speck GO:0016607 IDA
    spliceosomal complex GO:0005681 IDA
    Prp19 complex GO:0000974 IDA
 Experiment description of studies that identified U2AF2 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for U2AF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C1orf190  
Two-hybrid Homo sapiens
2 SMAD5 4090
Affinity Capture-MS Homo sapiens
3 U2AF1L2  
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
4 SFRS11  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
5 DVL2 1856
Two-hybrid Homo sapiens
6 GEMIN2 8487
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 SRPK2 6733
Two-hybrid Homo sapiens
8 SRSF1 6426
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 TCERG1  
Affinity Capture-MS Homo sapiens
10 SF1 7536
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 PUF60 22827
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
12 PRPF40A 55660
Affinity Capture-MS Homo sapiens
13 THAP1  
Two-hybrid Homo sapiens
14 DPPA2  
Two-hybrid Homo sapiens
15 RNPS1 10921
Affinity Capture-MS Homo sapiens
16 VCL 7414
Affinity Capture-MS Homo sapiens
17 WT1  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
18 BEND7 222389
Two-hybrid Homo sapiens
19 CHIC2  
Two-hybrid Homo sapiens
20 SFRS2IP  
Invivo Homo sapiens
Two-hybrid Homo sapiens
21 KIAA0907  
Two-hybrid Homo sapiens
22 U2AF1 7307
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
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