Gene description for PUF60
Gene name poly-U binding splicing factor 60KDa
Gene symbol PUF60
Other names/aliases FIR
RoBPI
SIAHBP1
VRJS
Species Homo sapiens
 Database cross references - PUF60
ExoCarta ExoCarta_22827
Entrez Gene 22827
HGNC 17042
MIM 604819
UniProt Q9UHX1  
 PUF60 identified in exosomes derived from the following tissue/cell type
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PUF60
Molecular Function
    DNA binding GO:0003677 IEA
    nucleotide binding GO:0000166 IEA
    identical protein binding GO:0042802 IPI
    poly(A) RNA binding GO:0044822 IDA
    protein binding GO:0005515 IPI
Biological Process
    mRNA processing GO:0006397 IEA
    transcription, DNA-templated GO:0006351 IEA
    regulation of transcription, DNA-templated GO:0006355 IEA
    RNA splicing GO:0008380 IEA
    apoptotic process GO:0006915 IEA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    cyclin-dependent protein kinase activating kinase holoenzyme complex GO:0019907 IDA
    Golgi apparatus GO:0005794 IDA
    nucleoplasm GO:0005654 IDA
    ribonucleoprotein complex GO:0030529 IEA
 Experiment description of studies that identified PUF60 in exosomes
1
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PUF60
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
2 ELAC2  
Affinity Capture-MS Homo sapiens
3 HCNGP  
Two-hybrid Homo sapiens
4 PABPC1 26986
Affinity Capture-MS Homo sapiens
5 KHSRP 8570
Affinity Capture-MS Homo sapiens
6 PABPC4 8761
Affinity Capture-MS Homo sapiens
7 UGP2 7360
Affinity Capture-MS Homo sapiens
8 CHD3  
Two-hybrid Homo sapiens
9 CD2BP2 10421
Affinity Capture-MS Homo sapiens
10 G3BP1 10146
Affinity Capture-MS Homo sapiens
11 SF3B1 23451
Affinity Capture-MS Homo sapiens
12 SFRS11  
Affinity Capture-MS Homo sapiens
13 HNRNPC 3183
Affinity Capture-MS Homo sapiens
14 SSB 6741
Affinity Capture-MS Homo sapiens
15 TROVE2 6738
Affinity Capture-MS Homo sapiens
16 HNRNPA3 220988
Affinity Capture-MS Homo sapiens
17 PUF60 22827
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 STOM 2040
Affinity Capture-MS Homo sapiens
19 LOH12CR1  
Two-hybrid Homo sapiens
20 PPP1R16A  
Two-hybrid Homo sapiens
21 DDX3X 1654
Affinity Capture-MS Homo sapiens
22 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
23 MOV10 4343
Affinity Capture-MS Homo sapiens
24 HNRNPL 3191
Affinity Capture-MS Homo sapiens
25 TTN 7273
Affinity Capture-MS Homo sapiens
26 RNPS1 10921
Affinity Capture-MS Homo sapiens
27 U2AF2 11338
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
28 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
29 P4HB 5034
Affinity Capture-MS Homo sapiens
30 SARS2  
Affinity Capture-MS Homo sapiens
31 ILK 3611
Affinity Capture-MS Homo sapiens
32 RNMTL1  
Affinity Capture-MS Homo sapiens
33 EWSR1 2130
Affinity Capture-MS Homo sapiens
34 VIM 7431
Two-hybrid Homo sapiens
35 OLA1 29789
Affinity Capture-MS Homo sapiens
36 IGF2BP2 10644
Affinity Capture-MS Homo sapiens
37 SF3A3 10946
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PUF60 is involved
No pathways found





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