Gene description for PUF60
Gene name poly-U binding splicing factor 60KDa
Gene symbol PUF60
Other names/aliases FIR
RoBPI
SIAHBP1
VRJS
Species Homo sapiens
 Database cross references - PUF60
ExoCarta ExoCarta_22827
Vesiclepedia VP_22827
Entrez Gene 22827
HGNC 17042
MIM 604819
UniProt Q9UHX1  
 PUF60 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
 Gene ontology annotations for PUF60
Molecular Function
    DNA binding GO:0003677 IEA
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    cadherin binding GO:0045296 HDA
Biological Process
    alternative mRNA splicing, via spliceosome GO:0000380 IBA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IBA
    mRNA splice site recognition GO:0006376 IBA
    apoptotic process GO:0006915 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cell junction GO:0030054 IDA
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified PUF60 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 407
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
12
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 405
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 417
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 411
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 413
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 406
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 415
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 408
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 409
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
31
Experiment ID 416
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
33
Experiment ID 410
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for PUF60
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 CRBN  
Affinity Capture-MS Homo sapiens
3 P4HB 5034
Affinity Capture-MS Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 HNRNPC 3183
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
6 ZG16  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 PABPC1 26986
Affinity Capture-MS Homo sapiens
8 SARS2 54938
Affinity Capture-MS Homo sapiens
9 DNAJC8 22826
Co-fractionation Homo sapiens
10 GUK1 2987
Co-fractionation Homo sapiens
11 TH  
Two-hybrid Homo sapiens
12 PKM 5315
Co-fractionation Homo sapiens
13 SRPK2 6733
Affinity Capture-MS Homo sapiens
14 SSB 6741
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SMNDC1  
Co-fractionation Homo sapiens
16 ACTC1 70
Proximity Label-MS Homo sapiens
17 HIRIP3  
Co-fractionation Homo sapiens
18 NOP56 10528
Proximity Label-MS Homo sapiens
19 FAM129B 64855
Co-fractionation Homo sapiens
20 KIF20A 10112
Affinity Capture-MS Homo sapiens
21 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
22 ARCN1 372
Co-fractionation Homo sapiens
23 CAND1 55832
Affinity Capture-MS Homo sapiens
24 PIR 8544
Affinity Capture-MS Homo sapiens
25 PRPF40A 55660
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
26 SNRPF 6636
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
27 SOX2  
Affinity Capture-MS Homo sapiens
28 PCBP3 54039
Two-hybrid Homo sapiens
29 VPS29 51699
Co-fractionation Homo sapiens
30 ILF2 3608
Co-fractionation Homo sapiens
31 CDKN1A  
Affinity Capture-MS Homo sapiens
32 GRB7 2886
Affinity Capture-MS Homo sapiens
33 DDX23 9416
Proximity Label-MS Homo sapiens
34 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
35 RNPS1 10921
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 B3GNT2 10678
Affinity Capture-MS Homo sapiens
37 DIAPH1 1729
Co-fractionation Homo sapiens
38 RAP1GDS1 5910
Two-hybrid Homo sapiens
39 KIF23 9493
Affinity Capture-MS Homo sapiens
40 MCM2 4171
Affinity Capture-MS Homo sapiens
41 DDB1 1642
Co-fractionation Homo sapiens
42 SF3B1 23451
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
43 SRSF10 10772
Affinity Capture-MS Homo sapiens
44 COPS5 10987
Affinity Capture-MS Homo sapiens
45 FBXW7  
Synthetic Lethality Homo sapiens
Affinity Capture-MS Homo sapiens
46 PRC1 9055
Affinity Capture-MS Homo sapiens
47 SRRM2 23524
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
48 YWHAG 7532
Affinity Capture-MS Homo sapiens
49 OBSL1 23363
Affinity Capture-MS Homo sapiens
50 C1qbp 12261
Affinity Capture-MS Mus musculus
51 SF3A2 8175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SUGP1  
Two-hybrid Homo sapiens
53 LAMTOR3 8649
Co-fractionation Homo sapiens
54 FAF1 11124
Co-fractionation Homo sapiens
55 ILK 3611
Affinity Capture-MS Homo sapiens
56 TRMT1 55621
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 GPN1  
Affinity Capture-MS Homo sapiens
58 MYCN  
Affinity Capture-MS Homo sapiens
59 SDCBP 6386
Two-hybrid Homo sapiens
60 CHD3 1107
Two-hybrid Homo sapiens
61 LOH12CR1 118426
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
62 SREK1 140890
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 PABPC4 8761
Affinity Capture-MS Homo sapiens
64 EEF1A1 1915
Co-fractionation Homo sapiens
65 UGP2 7360
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
66 KIF14 9928
Affinity Capture-MS Homo sapiens
67 EWSR1 2130
Affinity Capture-MS Homo sapiens
68 E2F4  
Affinity Capture-MS Homo sapiens
69 G3BP1 10146
Affinity Capture-MS Homo sapiens
70 FTHL17  
Two-hybrid Homo sapiens
71 RAD23B 5887
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
72 JMJD6 23210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 MPV17 4358
Co-fractionation Homo sapiens
74 USP10 9100
Affinity Capture-MS Homo sapiens
75 ZNF207 7756
Affinity Capture-MS Homo sapiens
76 PARK2  
Affinity Capture-MS Homo sapiens
77 STOM 2040
Affinity Capture-MS Homo sapiens
78 TRA2A 29896
Affinity Capture-MS Homo sapiens
79 KLC4 89953
Affinity Capture-MS Homo sapiens
80 PPP1R16A 84988
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
81 UBE2I 7329
Two-hybrid Homo sapiens
82 CRNKL1 51340
Affinity Capture-MS Homo sapiens
83 Ube2k 53323
Affinity Capture-MS Mus musculus
84 SNRPB 6628
Co-fractionation Homo sapiens
85 EP300 2033
Affinity Capture-MS Homo sapiens
86 CDH1 999
Proximity Label-MS Homo sapiens
87 CHMP4B 128866
Affinity Capture-MS Homo sapiens
88 FOXL2  
Affinity Capture-MS Homo sapiens
89 FUBP1 8880
Two-hybrid Homo sapiens
90 BARD1 580
Affinity Capture-MS Homo sapiens
91 TTN 7273
Affinity Capture-MS Homo sapiens
92 NTRK1 4914
Affinity Capture-MS Homo sapiens
93 FOXK2  
Affinity Capture-MS Homo sapiens
94 IK 3550
Affinity Capture-MS Homo sapiens
95 IFI16 3428
Affinity Capture-MS Homo sapiens
96 U2AF2 11338
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
97 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
98 ERP29 10961
Two-hybrid Homo sapiens
99 RAB2A 5862
Affinity Capture-MS Homo sapiens
100 SF3B6 51639
Co-fractionation Homo sapiens
101 Ddx42 72047
Two-hybrid Mus musculus
102 ZC3H14  
Affinity Capture-MS Homo sapiens
103 NELFE 7936
Affinity Capture-MS Homo sapiens
104 KIAA1683  
Two-hybrid Homo sapiens
105 EED  
Affinity Capture-MS Homo sapiens
106 ID3  
Two-hybrid Homo sapiens
107 SON 6651
Co-fractionation Homo sapiens
108 NUDC 10726
Co-fractionation Homo sapiens
109 HNRNPA3 220988
Affinity Capture-MS Homo sapiens
110 PPM1G 5496
Co-fractionation Homo sapiens
111 CEBPA  
Protein-peptide Homo sapiens
112 AIMP2 7965
Co-fractionation Homo sapiens
113 SF3A3 10946
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
114 OGT 8473
Reconstituted Complex Homo sapiens
115 SNRPB2 6629
Co-fractionation Homo sapiens
116 ANLN 54443
Affinity Capture-MS Homo sapiens
117 SRSF11 9295
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 TMPO 7112
Affinity Capture-MS Homo sapiens
119 OLA1 29789
Affinity Capture-MS Homo sapiens
120 PSMA3 5684
Affinity Capture-MS Homo sapiens
121 CACNA1A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
122 SF1 7536
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
123 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 METTL14  
Affinity Capture-MS Homo sapiens
125 CCNC  
Two-hybrid Homo sapiens
126 CLK3  
Affinity Capture-MS Homo sapiens
127 TIPRL 261726
Co-fractionation Homo sapiens
128 RC3H2  
Affinity Capture-MS Homo sapiens
129 ECT2 1894
Affinity Capture-MS Homo sapiens
130 SIRT7  
Affinity Capture-MS Homo sapiens
131 CD2BP2 10421
Affinity Capture-MS Homo sapiens
132 SRSF1 6426
Affinity Capture-MS Homo sapiens
133 SAP30BP  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
134 MECP2 4204
Affinity Capture-MS Homo sapiens
135 EGFR 1956
Negative Genetic Homo sapiens
136 ELAC2 60528
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
137 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
138 FOXL1  
Affinity Capture-MS Homo sapiens
139 METTL3  
Affinity Capture-MS Homo sapiens
140 FN1 2335
Affinity Capture-MS Homo sapiens
141 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
142 TROVE2 6738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
143 ZYX 7791
Co-fractionation Homo sapiens
144 CDK2 1017
Affinity Capture-MS Homo sapiens
145 EIF4H 7458
Co-fractionation Homo sapiens
146 PCBP1 5093
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
147 MREG  
Two-hybrid Homo sapiens
148 TADA2A  
Affinity Capture-MS Homo sapiens
149 MYC  
Affinity Capture-MS Homo sapiens
150 RPA3 6119
Proximity Label-MS Homo sapiens
151 VIM 7431
Two-hybrid Homo sapiens
152 SF3A1 10291
Affinity Capture-MS Homo sapiens
153 INO80B 83444
Affinity Capture-MS Homo sapiens
154 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
155 ABCC1 4363
Co-fractionation Homo sapiens
156 MATR3 9782
Co-fractionation Homo sapiens
157 CUL7 9820
Affinity Capture-MS Homo sapiens
158 DDRGK1 65992
Affinity Capture-MS Homo sapiens
159 FOXQ1  
Affinity Capture-MS Homo sapiens
160 ARGLU1  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
161 HSPD1 3329
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
162 MRPL38  
Affinity Capture-MS Homo sapiens
163 BMP4 652
Affinity Capture-MS Homo sapiens
164 UPF2 26019
Two-hybrid Homo sapiens
165 FHL2 2274
Two-hybrid Homo sapiens
166 PPME1 51400
Affinity Capture-MS Homo sapiens
167 EIF3H 8667
Affinity Capture-MS Homo sapiens
168 U2SURP 23350
Co-fractionation Homo sapiens
169 QRICH1  
Two-hybrid Homo sapiens
170 DDX46 9879
Affinity Capture-MS Homo sapiens
171 DHX15 1665
Co-fractionation Homo sapiens
172 DDX50 79009
Two-hybrid Homo sapiens
173 Ect2  
Affinity Capture-MS Mus musculus
174 SRRM1 10250
Affinity Capture-MS Homo sapiens
175 KIAA0907  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
176 RNMTL1  
Affinity Capture-MS Homo sapiens
177 PAPOLA 10914
Co-fractionation Homo sapiens
178 CPSF6 11052
Co-fractionation Homo sapiens
179 RPL6 6128
Co-fractionation Homo sapiens
180 FOLR1 2348
Affinity Capture-MS Homo sapiens
181 RBM25 58517
Co-fractionation Homo sapiens
182 GSK3A 2931
Affinity Capture-MS Homo sapiens
183 SNRPA1 6627
Co-fractionation Homo sapiens
184 ZNF746  
Affinity Capture-MS Homo sapiens
185 APEX1 328
Proximity Label-MS Homo sapiens
186 SNRPA 6626
Two-hybrid Homo sapiens
187 ARL8A 127829
Two-hybrid Homo sapiens
188 HNRNPM 4670
Co-fractionation Homo sapiens
189 THRAP3 9967
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
190 PUF60 22827
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
191 PTBP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
192 DDIT4L  
Two-hybrid Homo sapiens
193 AXIN1  
Affinity Capture-MS Homo sapiens
194 TARS 6897
Co-fractionation Homo sapiens
195 UFL1 23376
Affinity Capture-MS Homo sapiens
196 MOV10 4343
Affinity Capture-MS Homo sapiens
197 DDX3X 1654
Affinity Capture-MS Homo sapiens
198 NCL 4691
Affinity Capture-MS Homo sapiens
199 HNRNPL 3191
Affinity Capture-MS Homo sapiens
200 H3F3A 3020
Co-fractionation Homo sapiens
201 HNRNPH3 3189
Co-fractionation Homo sapiens
202 UBTFL1  
Two-hybrid Homo sapiens
203 KHSRP 8570
Affinity Capture-MS Homo sapiens
204 UPF1 5976
Affinity Capture-MS Homo sapiens
205 SRSF8 10929
Affinity Capture-MS Homo sapiens
206 SRPK1 6732
Affinity Capture-MS Homo sapiens
207 HNRNPF 3185
Co-fractionation Homo sapiens
208 ERG  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
209 SNW1 22938
Affinity Capture-MS Homo sapiens
210 KRAS 3845
Negative Genetic Homo sapiens
211 RC3H1 149041
Affinity Capture-MS Homo sapiens
212 U2af2 22185
Two-hybrid Mus musculus
213 GAPDH 2597
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
214 Tecpr2  
Affinity Capture-MS Mus musculus
215 MYO18A 399687
Co-fractionation Homo sapiens
216 IGF2BP2 10644
Affinity Capture-MS Homo sapiens
217 XPNPEP1 7511
Co-fractionation Homo sapiens
218 CASC3  
Affinity Capture-MS Homo sapiens
219 Apc  
Affinity Capture-MS Mus musculus
220 MED28  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
221 PNN 5411
Affinity Capture-MS Homo sapiens
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