Gene description for BMP4
Gene name bone morphogenetic protein 4
Gene symbol BMP4
Other names/aliases BMP2B
BMP2B1
MCOPS6
OFC11
ZYME
Species Homo sapiens
 Database cross references - BMP4
ExoCarta ExoCarta_652
Vesiclepedia VP_652
Entrez Gene 652
HGNC 1071
MIM 112262
UniProt P12644  
 BMP4 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
 Gene ontology annotations for BMP4
Molecular Function
    cytokine activity GO:0005125 IBA
    cytokine activity GO:0005125 IDA
    protein binding GO:0005515 IPI
    growth factor activity GO:0008083 IEA
    heparin binding GO:0008201 IEA
    co-receptor binding GO:0039706 IPI
    chemoattractant activity GO:0042056 IDA
    protein serine/threonine kinase activator activity GO:0043539 IDA
    BMP receptor binding GO:0070700 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    osteoblast differentiation GO:0001649 IDA
    metanephros development GO:0001656 IEA
    ureteric bud development GO:0001657 IDA
    branching involved in ureteric bud morphogenesis GO:0001658 IDA
    kidney development GO:0001822 IMP
    mesonephros development GO:0001823 IEP
    neural tube closure GO:0001843 IEA
    positive regulation of protein phosphorylation GO:0001934 IDA
    positive regulation of endothelial cell proliferation GO:0001938 IEA
    endochondral ossification GO:0001958 ISS
    blood vessel endothelial cell proliferation involved in sprouting angiogenesis GO:0002043 IDA
    chondrocyte differentiation GO:0002062 ISS
    hematopoietic progenitor cell differentiation GO:0002244 IDA
    lymphoid progenitor cell differentiation GO:0002320 IMP
    heart morphogenesis GO:0003007 IMP
    renal system process GO:0003014 IEA
    heart induction GO:0003129 IMP
    secondary heart field specification GO:0003139 IMP
    outflow tract septum morphogenesis GO:0003148 ISS
    membranous septum morphogenesis GO:0003149 ISS
    muscular septum morphogenesis GO:0003150 ISS
    sinoatrial node development GO:0003163 NAS
    aortic valve morphogenesis GO:0003180 ISS
    pulmonary valve morphogenesis GO:0003184 ISS
    endocardial cushion development GO:0003197 ISS
    epithelial to mesenchymal transition involved in endocardial cushion formation GO:0003198 ISS
    cardiac right ventricle morphogenesis GO:0003215 ISS
    apoptotic process involved in endocardial cushion morphogenesis GO:0003277 ISS
    cardiac septum development GO:0003279 TAS
    type B pancreatic cell development GO:0003323 IDA
    transcription by RNA polymerase II GO:0006366 IEA
    germ cell development GO:0007281 IEA
    endoderm development GO:0007492 IEA
    mesodermal cell fate determination GO:0007500 IEA
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 ISS
    negative regulation of cell population proliferation GO:0008285 IDA
    negative regulation of cell population proliferation GO:0008285 IMP
    post-embryonic development GO:0009791 IDA
    anterior/posterior axis specification GO:0009948 IEA
    specification of animal organ position GO:0010159 IEA
    regulation of cell fate commitment GO:0010453 IDA
    positive regulation of gene expression GO:0010628 IDA
    negative regulation of gene expression GO:0010629 IDA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IEA
    telencephalon development GO:0021537 IDA
    dorsal/ventral neural tube patterning GO:0021904 IEA
    telencephalon regionalization GO:0021978 IEA
    pituitary gland development GO:0021983 IEA
    erythrocyte differentiation GO:0030218 IEA
    monocyte differentiation GO:0030224 IDA
    macrophage differentiation GO:0030225 IDA
    positive regulation of bone mineralization GO:0030501 IDA
    positive regulation of bone mineralization GO:0030501 IDA
    BMP signaling pathway GO:0030509 IDA
    BMP signaling pathway GO:0030509 IMP
    BMP signaling pathway GO:0030509 IMP
    BMP signaling pathway GO:0030509 ISS
    positive regulation of BMP signaling pathway GO:0030513 ISS
    positive regulation of protein binding GO:0032092 IDA
    negative regulation of chondrocyte differentiation GO:0032331 IEA
    positive regulation of collagen biosynthetic process GO:0032967 IDA
    negative regulation of T cell differentiation in thymus GO:0033085 IMP
    negative regulation of immature T cell proliferation in thymus GO:0033088 IMP
    embryonic hindlimb morphogenesis GO:0035116 IEA
    tendon cell differentiation GO:0035990 ISS
    deltoid tuberosity development GO:0035993 ISS
    ameloblast differentiation GO:0036305 IEA
    regulation of protein import into nucleus GO:0042306 IDA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    odontogenesis GO:0042476 IGI
    regulation of odontogenesis of dentin-containing tooth GO:0042487 IEA
    inner ear auditory receptor cell differentiation GO:0042491 IEA
    embryonic digit morphogenesis GO:0042733 IEA
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of programmed cell death GO:0043068 IDA
    positive regulation of endothelial cell differentiation GO:0045603 IEA
    positive regulation of epidermal cell differentiation GO:0045606 IDA
    negative regulation of myoblast differentiation GO:0045662 IDA
    positive regulation of neuron differentiation GO:0045666 IEA
    positive regulation of osteoblast differentiation GO:0045669 IDA
    positive regulation of osteoblast differentiation GO:0045669 IDA
    positive regulation of ossification GO:0045778 IDA
    negative regulation of cell cycle GO:0045786 IDA
    negative regulation of mitotic nuclear division GO:0045839 IDA
    negative regulation of striated muscle tissue development GO:0045843 IDA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    lung alveolus development GO:0048286 IDA
    intermediate mesodermal cell differentiation GO:0048392 IDA
    positive regulation of smooth muscle cell proliferation GO:0048661 IDA
    neuron fate commitment GO:0048663 IEA
    embryonic cranial skeleton morphogenesis GO:0048701 IEA
    smooth muscle tissue development GO:0048745 IEP
    smooth muscle tissue development GO:0048745 ISS
    stem cell differentiation GO:0048863 ISS
    positive regulation of epithelial cell proliferation GO:0050679 IDA
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    positive chemotaxis GO:0050918 IEA
    smooth muscle cell differentiation GO:0051145 IEA
    regulation of smooth muscle cell differentiation GO:0051150 IEA
    cardiac muscle cell differentiation GO:0055007 IEA
    positive regulation of cardiac muscle fiber development GO:0055020 IMP
    lens induction in camera-type eye GO:0060235 IEA
    embryonic skeletal joint morphogenesis GO:0060272 IEA
    cranial suture morphogenesis GO:0060363 IEA
    positive regulation of SMAD protein signal transduction GO:0060391 IDA
    positive regulation of SMAD protein signal transduction GO:0060391 IMP
    lung morphogenesis GO:0060425 IDA
    lung vasculature development GO:0060426 IMP
    bronchus development GO:0060433 IDA
    trachea development GO:0060438 IDA
    trachea formation GO:0060440 IEA
    epithelial tube branching involved in lung morphogenesis GO:0060441 IDA
    branching involved in prostate gland morphogenesis GO:0060442 IEA
    bud elongation involved in lung branching GO:0060449 IEA
    epithelial cell proliferation involved in lung morphogenesis GO:0060502 IDA
    bud dilation involved in lung branching GO:0060503 IDA
    prostatic bud formation GO:0060513 IEA
    mammary gland formation GO:0060592 IEA
    epithelial-mesenchymal cell signaling GO:0060684 IEA
    negative regulation of prostatic bud formation GO:0060686 IEA
    regulation of branching involved in prostate gland morphogenesis GO:0060687 IEA
    coronary vasculature development GO:0060976 ISS
    positive regulation of cartilage development GO:0061036 IDA
    positive regulation of branching involved in lung morphogenesis GO:0061047 ISS
    pharyngeal arch artery morphogenesis GO:0061626 ISS
    negative regulation of thymocyte apoptotic process GO:0070244 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    cellular response to BMP stimulus GO:0071773 IMP
    glomerular capillary formation GO:0072104 ISS
    negative regulation of glomerular mesangial cell proliferation GO:0072125 IDA
    nephric duct formation GO:0072179 IDA
    ureter morphogenesis GO:0072197 IEA
    negative regulation of mesenchymal cell proliferation involved in ureter development GO:0072200 IDA
    negative regulation of branching involved in ureteric bud morphogenesis GO:0090191 IDA
    negative regulation of glomerulus development GO:0090194 IDA
    positive regulation of protein localization to nucleus GO:1900182 ISS
    positive regulation of p38MAPK cascade GO:1900745 IDA
    positive regulation of odontoblast differentiation GO:1901331 ISS
    regulation of miRNA transcription GO:1902893 IMP
    negative regulation of miRNA transcription GO:1902894 IDA
    pericyte cell differentiation GO:1904238 IEA
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 TAS
    negative regulation of vascular associated smooth muscle cell migration GO:1904753 TAS
    cardiac jelly development GO:1905072 ISS
    positive regulation of cardiac neural crest cell migration involved in outflow tract morphogenesis GO:1905312 IEA
    regulation of mesodermal cell differentiation GO:1905770 IEA
    negative regulation of metanephric S-shaped body morphogenesis GO:2000005 IDA
    negative regulation of metanephric comma-shaped body morphogenesis GO:2000007 IDA
    positive regulation of primary miRNA processing GO:2000636 IDA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 ISS
Subcellular Localization
    extracellular region GO:0005576 ISS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISS
    endoplasmic reticulum lumen GO:0005788 TAS
 Experiment description of studies that identified BMP4 in exosomes
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for BMP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL11 6135
Affinity Capture-MS Homo sapiens
2 SUFU 51684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RPS3A 6189
Affinity Capture-MS Homo sapiens
4 SRPK2 6733
Affinity Capture-MS Homo sapiens
5 CFC1  
Affinity Capture-MS Homo sapiens
6 FGF4  
Affinity Capture-MS Homo sapiens
7 PROS1 5627
Affinity Capture-MS Homo sapiens
8 RPL10 6134
Affinity Capture-MS Homo sapiens
9 TOP1 7150
Affinity Capture-MS Homo sapiens
10 RPL14 9045
Affinity Capture-MS Homo sapiens
11 MVK 4598
Affinity Capture-MS Homo sapiens
12 IL20  
Affinity Capture-MS Homo sapiens
13 APOD 347
Affinity Capture-MS Homo sapiens
14 RPS3 6188
Affinity Capture-MS Homo sapiens
15 DIMT1 27292
Affinity Capture-MS Homo sapiens
16 RPS20 6224
Affinity Capture-MS Homo sapiens
17 CLU 1191
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 UBE2O 63893
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 H1FX 8971
Affinity Capture-MS Homo sapiens
20 SOSTDC1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 EIF4A3 9775
Affinity Capture-MS Homo sapiens
22 RPL36 25873
Affinity Capture-MS Homo sapiens
23 NPM1 4869
Affinity Capture-MS Homo sapiens
24 SRRM2 23524
Affinity Capture-MS Homo sapiens
25 KHDRBS1 10657
Affinity Capture-MS Homo sapiens
26 FBL 2091
Affinity Capture-MS Homo sapiens
27 EWSR1 2130
Affinity Capture-MS Homo sapiens
28 RPS9 6203
Affinity Capture-MS Homo sapiens
29 BMPR1B 658
Reconstituted Complex Homo sapiens
30 NMU  
Affinity Capture-MS Homo sapiens
31 LIPG 9388
Affinity Capture-MS Homo sapiens
32 P3H1 64175
Affinity Capture-MS Homo sapiens
33 CALR 811
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 ZFYVE9  
Two-hybrid Homo sapiens
35 RPL19 6143
Affinity Capture-MS Homo sapiens
36 RPS23 6228
Affinity Capture-MS Homo sapiens
37 RPL27 6155
Affinity Capture-MS Homo sapiens
38 RPL9 6133
Affinity Capture-MS Homo sapiens
39 RPL4 6124
Affinity Capture-MS Homo sapiens
40 CEACAM21  
Affinity Capture-MS Homo sapiens
41 MMP3 4314
Affinity Capture-MS Homo sapiens
42 MMP10 4319
Affinity Capture-MS Homo sapiens
43 STAU1 6780
Affinity Capture-MS Homo sapiens
44 RPL5 6125
Affinity Capture-MS Homo sapiens
45 RPL18A 6142
Affinity Capture-MS Homo sapiens
46 PABPC1 26986
Affinity Capture-MS Homo sapiens
47 SDF2L1 23753
Affinity Capture-MS Homo sapiens
48 CGREF1 10669
Affinity Capture-MS Homo sapiens
49 RPL31 6160
Affinity Capture-MS Homo sapiens
50 NXPH2  
Affinity Capture-MS Homo sapiens
51 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
52 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
53 DNAJA1 3301
Affinity Capture-MS Homo sapiens
54 CLEC11A 6320
Affinity Capture-MS Homo sapiens
55 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
56 RPL27A 6157
Affinity Capture-MS Homo sapiens
57 TDGF1  
Reconstituted Complex Homo sapiens
58 DHX9 1660
Affinity Capture-MS Homo sapiens
59 RPLP0 6175
Affinity Capture-MS Homo sapiens
60 RPS15A 6210
Affinity Capture-MS Homo sapiens
61 CORT  
Affinity Capture-MS Homo sapiens
62 SRPK1 6732
Affinity Capture-MS Homo sapiens
63 RGMB  
Reconstituted Complex Homo sapiens
64 TWSG1  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
65 DDX18 8886
Affinity Capture-MS Homo sapiens
66 HSPA5 3309
Affinity Capture-MS Homo sapiens
67 RBM39 9584
Affinity Capture-MS Homo sapiens
68 MAP4 4134
Affinity Capture-MS Homo sapiens
69 UPF1 5976
Affinity Capture-MS Homo sapiens
70 CALR3  
Affinity Capture-MS Homo sapiens
71 TNFSF8  
Affinity Capture-MS Homo sapiens
72 ZNF622 90441
Affinity Capture-MS Homo sapiens
73 CBLN4  
Affinity Capture-MS Homo sapiens
74 CST8  
Affinity Capture-MS Homo sapiens
75 PRG2  
Affinity Capture-MS Homo sapiens
76 ACVR2A  
Reconstituted Complex Homo sapiens
77 RPS8 6202
Affinity Capture-MS Homo sapiens
78 IFNE  
Affinity Capture-MS Homo sapiens
79 RPL3 6122
Affinity Capture-MS Homo sapiens
80 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
81 DCAF16 54876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 IL5RA  
Affinity Capture-MS Homo sapiens
83 SART1 9092
Affinity Capture-MS Homo sapiens
84 SLC22A23  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 PCBP1 5093
Affinity Capture-MS Homo sapiens
86 BMPR2 659
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
87 CRLF1 9244
Affinity Capture-MS Homo sapiens
88 RPS13 6207
Affinity Capture-MS Homo sapiens
89 FBXO2 26232
Affinity Capture-MS Homo sapiens
90 RPL35A 6165
Affinity Capture-MS Homo sapiens
91 RPL12 6136
Affinity Capture-MS Homo sapiens
92 DDX54 79039
Affinity Capture-MS Homo sapiens
93 RPL22 6146
Affinity Capture-MS Homo sapiens
94 RPL23A 6147
Affinity Capture-MS Homo sapiens
95 RPL18 6141
Affinity Capture-MS Homo sapiens
96 RPS11 6205
Affinity Capture-MS Homo sapiens
97 RPL21 6144
Affinity Capture-MS Homo sapiens
98 RPL7 6129
Affinity Capture-MS Homo sapiens
99 PDP1 54704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 RPL26 6154
Affinity Capture-MS Homo sapiens
101 KIAA1522 57648
Affinity Capture-MS Homo sapiens
102 RPL13 6137
Affinity Capture-MS Homo sapiens
103 YBX1 4904
Affinity Capture-MS Homo sapiens
104 HS3ST1  
Affinity Capture-MS Homo sapiens
105 RPL6 6128
Affinity Capture-MS Homo sapiens
106 METRN 79006
Affinity Capture-MS Homo sapiens
107 HNRNPM 4670
Affinity Capture-MS Homo sapiens
108 PTBP1 5725
Affinity Capture-MS Homo sapiens
109 MRPS15  
Affinity Capture-MS Homo sapiens
110 BMPR1A 657
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
111 PUF60 22827
Affinity Capture-MS Homo sapiens
112 EDDM3A  
Affinity Capture-MS Homo sapiens
113 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
114 GTPBP4 23560
Affinity Capture-MS Homo sapiens
115 RPL23 9349
Affinity Capture-MS Homo sapiens
116 RPS6 6194
Affinity Capture-MS Homo sapiens
117 RPL8 6132
Affinity Capture-MS Homo sapiens
118 RPL28 6158
Affinity Capture-MS Homo sapiens
119 HNF1B  
Affinity Capture-MS Homo sapiens
120 HNRNPU 3192
Affinity Capture-MS Homo sapiens
121 RPS7 6201
Affinity Capture-MS Homo sapiens
122 GPX7 2882
Affinity Capture-MS Homo sapiens
123 FXR2 9513
Affinity Capture-MS Homo sapiens
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