Gene description for BMP4
Gene name bone morphogenetic protein 4
Gene symbol BMP4
Other names/aliases BMP2B
BMP2B1
MCOPS6
OFC11
ZYME
Species Homo sapiens
 Database cross references - BMP4
ExoCarta ExoCarta_652
Vesiclepedia VP_652
Entrez Gene 652
HGNC 1071
MIM 112262
UniProt P12644  
 BMP4 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
 Gene ontology annotations for BMP4
Molecular Function
    chemoattractant activity GO:0042056 IDA
    growth factor activity GO:0008083 IEA
    transforming growth factor beta receptor binding GO:0005160 IBA
    heparin binding GO:0008201 IEA
    BMP receptor binding GO:0070700 IDA
    cytokine activity GO:0005125 IDA
    protein binding GO:0005515 IPI
Biological Process
    endocardial cushion development GO:0003197 TAS
    cranial suture morphogenesis GO:0060363 IEA
    odontogenesis GO:0042476 IGI
    chondrocyte differentiation GO:0002062 ISS
    epithelial-mesenchymal cell signaling GO:0060684 IEA
    positive regulation of DNA-dependent DNA replication GO:2000105 IDA
    negative regulation of apoptotic process GO:0043066 IDA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    negative regulation of cell proliferation involved in heart morphogenesis GO:2000137 IMP
    lung morphogenesis GO:0060425 IDA
    steroid hormone mediated signaling pathway GO:0043401 IMP
    cardiac septum development GO:0003279 TAS
    mesenchymal cell proliferation involved in ureteric bud development GO:0072138 IEA
    activation of MAPKK activity GO:0000186 IDA
    anterior/posterior axis specification GO:0009948 IEA
    monocyte differentiation GO:0030224 IDA
    renal system development GO:0072001 IEP
    osteoblast differentiation GO:0001649 IDA
    type B pancreatic cell development GO:0003323 IDA
    pulmonary artery endothelial tube morphogenesis GO:0061155 IDA
    kidney development GO:0001822 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    telencephalon regionalization GO:0021978 IEA
    bronchus development GO:0060433 IDA
    lymphoid progenitor cell differentiation GO:0002320 IMP
    telencephalon development GO:0021537 IDA
    negative regulation of thymocyte apoptotic process GO:0070244 IMP
    tendon cell differentiation GO:0035990 ISS
    negative regulation of T cell differentiation in thymus GO:0033085 IMP
    smoothened signaling pathway GO:0007224 IEP
    positive regulation of SMAD protein import into nucleus GO:0060391 IDA
    bud elongation involved in lung branching GO:0060449 IEA
    regulation of cell fate commitment GO:0010453 IDA
    negative regulation of striated muscle tissue development GO:0045843 IDA
    cellular response to growth factor stimulus GO:0071363 IEA
    negative regulation of MAP kinase activity GO:0043407 IDA
    specification of organ position GO:0010159 IEA
    ureter smooth muscle cell differentiation GO:0072193 ISS
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    positive regulation of branching involved in lung morphogenesis GO:0061047 ISS
    regulation of branching involved in prostate gland morphogenesis GO:0060687 IEA
    regulation of smooth muscle cell differentiation GO:0051150 IEA
    negative regulation of branching involved in ureteric bud morphogenesis GO:0090191 IDA
    glomerular capillary formation GO:0072104 ISS
    positive regulation of ossification GO:0045778 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    lung alveolus development GO:0048286 IDA
    regulation of protein import into nucleus GO:0042306 IDA
    mesenchymal cell proliferation involved in ureter development GO:0072198 IEA
    negative regulation of glomerular mesangial cell proliferation GO:0072125 IDA
    negative regulation of cell cycle GO:0045786 IDA
    branching morphogenesis of an epithelial tube GO:0048754 IDA
    secondary heart field specification GO:0003139 IMP
    hematopoietic progenitor cell differentiation GO:0002244 IDA
    mesenchymal cell differentiation involved in kidney development GO:0072161 IEA
    extracellular matrix organization GO:0030198 TAS
    negative regulation of prostatic bud formation GO:0060686 IEA
    epithelial cell proliferation involved in lung morphogenesis GO:0060502 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    endochondral ossification GO:0001958 ISS
    BMP signaling pathway involved in renal system segmentation GO:0061151 ISS
    ureteric bud development GO:0001657 IDA
    negative regulation of cell death GO:0060548 IDA
    trachea formation GO:0060440 IEA
    positive regulation of neuron differentiation GO:0045666 IEA
    embryonic skeletal joint morphogenesis GO:0060272 IEA
    trachea development GO:0060438 IDA
    neural tube closure GO:0001843 IEA
    SMAD protein signal transduction GO:0060395 IDA
    renal system process GO:0003014 IEA
    positive regulation of bone mineralization GO:0030501 IDA
    protein localization to nucleus GO:0034504 IDA
    regulation of pathway-restricted SMAD protein phosphorylation GO:0060393 IDA
    mesodermal cell fate determination GO:0007500 IEA
    positive regulation of endothelial cell differentiation GO:0045603 IEA
    branching involved in ureteric bud morphogenesis GO:0001658 IDA
    positive regulation of collagen biosynthetic process GO:0032967 IDA
    positive regulation of kidney development GO:0090184 IDA
    positive regulation of pathway-restricted SMAD protein phosphorylation GO:0010862 IMP
    positive regulation of epidermal cell differentiation GO:0045606 IDA
    mammary gland formation GO:0060592 IEA
    regulation of odontogenesis of dentin-containing tooth GO:0042487 IEA
    positive regulation of cartilage development GO:0061036 IDA
    intermediate mesodermal cell differentiation GO:0048392 IDA
    positive regulation of protein binding GO:0032092 IDA
    embryonic hindlimb morphogenesis GO:0035116 IEA
    specification of ureteric bud anterior/posterior symmetry by BMP signaling pathway GO:0072101 IDA
    common-partner SMAD protein phosphorylation GO:0007182 IDA
    cloacal septation GO:0060197 IEA
    epithelial tube branching involved in lung morphogenesis GO:0060441 IDA
    negative regulation of mesenchymal cell proliferation involved in ureter development GO:0072200 IDA
    positive regulation of endothelial cell migration GO:0010595 IDA
    lens induction in camera-type eye GO:0060235 IEA
    embryonic cranial skeleton morphogenesis GO:0048701 IEA
    negative regulation of metanephric S-shaped body morphogenesis GO:2000005 IDA
    positive regulation of epithelial cell proliferation GO:0050679 IDA
    positive regulation of cell death GO:0010942 IDA
    blood vessel endothelial cell proliferation involved in sprouting angiogenesis GO:0002043 IDA
    BMP signaling pathway involved in ureter morphogenesis GO:0061149 ISS
    branching involved in prostate gland morphogenesis GO:0060442 IEA
    erythrocyte differentiation GO:0030218 IEA
    neuron fate commitment GO:0048663 IEA
    pituitary gland development GO:0021983 IEA
    BMP signaling pathway involved in nephric duct formation GO:0071893 IDA
    mesenchymal to epithelial transition involved in metanephros morphogenesis GO:0003337 IDA
    negative regulation of myoblast differentiation GO:0045662 IDA
    positive regulation of osteoblast differentiation GO:0045669 IDA
    mesonephros development GO:0001823 IEP
    negative regulation of phosphorylation GO:0042326 IDA
    metanephric collecting duct development GO:0072205 ISS
    smooth muscle tissue development GO:0048745 ISS
    bud dilation involved in lung branching GO:0060503 IDA
    glomerular visceral epithelial cell development GO:0072015 IEA
    negative regulation of immature T cell proliferation in thymus GO:0033088 IMP
    positive chemotaxis GO:0050918 IDA
    BMP signaling pathway GO:0030509 IMP
    BMP signaling pathway involved in heart induction GO:0003130 IMP
    dorsal/ventral neural tube patterning GO:0021904 IEA
    negative regulation of metanephric comma-shaped body morphogenesis GO:2000007 IDA
    positive regulation of endothelial cell proliferation GO:0001938 IDA
    germ cell development GO:0007281 IEA
    negative regulation of branch elongation involved in ureteric bud branching by BMP signaling pathway GO:0072097 IDA
    post-embryonic development GO:0009791 IDA
    negative regulation of cell proliferation GO:0008285 IDA
    positive regulation of BMP signaling pathway GO:0030513 ISS
    macrophage differentiation GO:0030225 IDA
    negative regulation of glomerulus development GO:0090194 IDA
    negative regulation of chondrocyte differentiation GO:0032331 IEA
    positive regulation of protein phosphorylation GO:0001934 IDA
    ureter epithelial cell differentiation GO:0072192 ISS
    negative regulation of mitotic nuclear division GO:0045839 IDA
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    embryonic digit morphogenesis GO:0042733 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    deltoid tuberosity development GO:0035993 ISS
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of smooth muscle cell proliferation GO:0048661 IDA
    inner ear receptor cell differentiation GO:0060113 IEA
    positive regulation of cardiac muscle fiber development GO:0055020 IMP
Subcellular Localization
    extracellular space GO:0005615 ISS
    extracellular region GO:0005576 TAS
    proteinaceous extracellular matrix GO:0005578 IEA
 Experiment description of studies that identified BMP4 in exosomes
1
Experiment ID 207
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for BMP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZFYVE9  
Affinity Capture-MS Homo sapiens
2 NOG 9241
Invivo Homo sapiens
3 BMPR2 659
Affinity Capture-Western Homo sapiens
4 BMP4 652
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
5 BMPR1A 657
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
6 BMPR1B 658
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
7 SOSTDC1  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which BMP4 is involved
PathwayEvidenceSource
Molecules associated with elastic fibres TAS Reactome





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