Gene description for SUFU
Gene name suppressor of fused homolog (Drosophila)
Gene symbol SUFU
Other names/aliases PRO1280
SUFUH
SUFUXL
Species Homo sapiens
 Database cross references - SUFU
ExoCarta ExoCarta_51684
Vesiclepedia VP_51684
Entrez Gene 51684
HGNC 16466
MIM 607035
UniProt Q9UMX1  
 SUFU identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SUFU
Molecular Function
    transcription corepressor activity GO:0003714 TAS
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IEA
    protein kinase binding GO:0019901 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    neural tube closure GO:0001843 IEA
    heart looping GO:0001947 IEA
    ventricular septum development GO:0003281 IEA
    regulation of DNA-templated transcription GO:0006355 IDA
    regulation of DNA-templated transcription GO:0006355 ISS
    regulation of DNA-templated transcription GO:0006355 TAS
    signal transduction GO:0007165 TAS
    spermatid development GO:0007286 IEA
    smoothened signaling pathway involved in ventral spinal cord interneuron specification GO:0021775 IEA
    smoothened signaling pathway involved in spinal cord motor neuron cell fate specification GO:0021776 IEA
    aorta development GO:0035904 IEA
    negative regulation of protein import into nucleus GO:0042308 TAS
    negative regulation of DNA-binding transcription factor activity GO:0043433 TAS
    skin development GO:0043588 IEA
    negative regulation of osteoblast differentiation GO:0045668 TAS
    negative regulation of smoothened signaling pathway GO:0045879 IBA
    negative regulation of smoothened signaling pathway GO:0045879 IMP
    negative regulation of smoothened signaling pathway GO:0045879 TAS
    coronary vasculature development GO:0060976 IEA
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 IEA
    positive regulation of cellular response to drug GO:2001040 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IMP
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IMP
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    ciliary tip GO:0097542 TAS
    ciliary base GO:0097546 TAS
    GLI-SUFU complex GO:1990788 IPI
    GLI-SUFU complex GO:1990788 ISS
 Experiment description of studies that identified SUFU in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SUFU
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCNO  
Affinity Capture-MS Homo sapiens
2 ZNF114 163071
Two-hybrid Homo sapiens
3 ZNF747  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 PEX26  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 SUFU 51684
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ARHGAP12  
Affinity Capture-MS Homo sapiens
8 CRTC2 200186
Two-hybrid Homo sapiens
9 LIPT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 LRRC36  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HOOK1  
Proximity Label-MS Homo sapiens
12 OVOL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ZNF785  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ZNF763  
Two-hybrid Homo sapiens
15 ARHGAP9 64333
Affinity Capture-MS Homo sapiens
16 NYAP2  
Affinity Capture-MS Homo sapiens
17 MYEOV  
Affinity Capture-MS Homo sapiens
18 SLC41A3 54946
Two-hybrid Homo sapiens
19 AES 166
Two-hybrid Homo sapiens
20 PCK2 5106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 FXR1 8087
Two-hybrid Homo sapiens
22 ZNF330  
Two-hybrid Homo sapiens
23 MID1IP1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
24 SAP18 10284
Affinity Capture-Western Homo sapiens
25 ALDH1B1 219
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 GLIS3  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
27 EDEM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CUL3 8452
Affinity Capture-MS Homo sapiens
29 FAM173B  
Affinity Capture-MS Homo sapiens
30 LOXL3 84695
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ZSCAN18  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
32 BFSP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
33 GLI1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
34 QPRT 23475
Two-hybrid Homo sapiens
35 ZNF764 92595
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 STX19  
Two-hybrid Homo sapiens
37 C6orf15  
Affinity Capture-MS Homo sapiens
38 ZIC1  
Two-hybrid Homo sapiens
39 DISC1 27185
Two-hybrid Homo sapiens
40 KRT27 342574
Two-hybrid Homo sapiens
41 ABI3 51225
Two-hybrid Homo sapiens
42 CRX  
Two-hybrid Homo sapiens
43 ZNF446  
Two-hybrid Homo sapiens
44 CDC25C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 MAGEL2  
Two-hybrid Homo sapiens
46 ZNF213  
Affinity Capture-MS Homo sapiens
47 HDX  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
48 L3MBTL3  
Two-hybrid Homo sapiens
49 STK36  
Co-localization Homo sapiens
50 SH3RF1  
Two-hybrid Homo sapiens
51 GLI2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
52 RCN3 57333
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
53 GLI3 2737
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
54 ZBTB46  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 KRT75 9119
Two-hybrid Homo sapiens
56 KDM5C  
Affinity Capture-MS Homo sapiens
57 NR2E1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 MEOX2  
Two-hybrid Homo sapiens
59 BICD2 23299
Proximity Label-MS Homo sapiens
60 BICD1 636
Proximity Label-MS Homo sapiens
61 NXPH3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 UBE3D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 NFATC3 4775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 FAM90A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 LCA5  
Two-hybrid Homo sapiens
67 MACROD2  
Co-fractionation Homo sapiens
68 BMP4 652
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 FBXL17  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
70 PRPF31 26121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 PEX11A  
Affinity Capture-MS Homo sapiens
72 IGF2 3481
Affinity Capture-MS Homo sapiens
73 ZNF263  
Two-hybrid Homo sapiens
74 Glis3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
75 TTLL3 26140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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