Gene description for SUFU
Gene name suppressor of fused homolog (Drosophila)
Gene symbol SUFU
Other names/aliases PRO1280
SUFUH
SUFUXL
Species Homo sapiens
 Database cross references - SUFU
ExoCarta ExoCarta_51684
Vesiclepedia VP_51684
Entrez Gene 51684
HGNC 16466
MIM 607035
UniProt Q9UMX1  
 SUFU identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SUFU
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    beta-catenin binding GO:0008013 IEA
    transcription corepressor activity GO:0003714 TAS
    signal transducer activity GO:0004871 TAS
    transcription factor binding GO:0008134 IDA
Biological Process
    negative regulation of osteoblast differentiation GO:0045668 TAS
    skeletal system development GO:0001501 TAS
    cytoplasmic sequestering of transcription factor GO:0042994 IEA
    multicellular organismal development GO:0007275 TAS
    negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:2000059 IEA
    regulation of transcription, DNA-templated GO:0006355 TAS
    negative regulation of transcription factor import into nucleus GO:0042992 TAS
    negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning GO:1901621 IEA
    signal transduction GO:0007165 TAS
    negative regulation of smoothened signaling pathway GO:0045879 TAS
    neural tube closure GO:0001843 IEA
    skin development GO:0043588 IEA
    smoothened signaling pathway involved in ventral spinal cord interneuron specification GO:0021775 IEA
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 TAS
    heart looping GO:0001947 IEA
    proteolysis GO:0006508 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    smoothened signaling pathway involved in spinal cord motor neuron cell fate specification GO:0021776 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    ciliary base GO:0097546 TAS
    nucleoplasm GO:0005654 IDA
    ciliary tip GO:0097542 TAS
    cytosol GO:0005829 TAS
    primary cilium GO:0072372 IEA
    cytoplasm GO:0005737 NAS
 Experiment description of studies that identified SUFU in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SUFU
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF764 92595
Two-hybrid Homo sapiens
2 RCN3 57333
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 MID1IP1  
Two-hybrid Homo sapiens
4 SUFU 51684
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
5 LGALS3 3958
Affinity Capture-MS Homo sapiens
6 SAP18 10284
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 GLIS3  
Two-hybrid Homo sapiens
8 BTRC 8945
Affinity Capture-MS Homo sapiens
9 HMGA1 3159
Affinity Capture-MS Homo sapiens
10 GLI2  
Reconstituted Complex Homo sapiens
11 GLI1  
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 QPRT 23475
Two-hybrid Homo sapiens
13 STK36  
Invivo Homo sapiens
14 FLJ20626  
Two-hybrid Homo sapiens
15 ZNF263  
Two-hybrid Homo sapiens
16 GLI3 2737
Reconstituted Complex Homo sapiens
17 PEX26  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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