Gene description for NFATC3
Gene name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Gene symbol NFATC3
Other names/aliases NFAT4
NFATX
Species Homo sapiens
 Database cross references - NFATC3
ExoCarta ExoCarta_4775
Vesiclepedia VP_4775
Entrez Gene 4775
HGNC 7777
MIM 602698
UniProt Q12968  
 NFATC3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NFATC3
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IGI
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IMP
    protein binding GO:0005515 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    regulation of transcription by RNA polymerase II GO:0006357 TAS
    protein import into nucleus GO:0006606 IEA
    inflammatory response GO:0006954 TAS
    positive regulation of cardiac muscle hypertrophy GO:0010613 IEA
    calcineurin-NFAT signaling cascade GO:0033173 IBA
    positive regulation of apoptotic process GO:0043065 IEA
    positive thymic T cell selection GO:0045059 ISS
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    negative regulation of miRNA transcription GO:1902894 IGI
    DN4 thymocyte differentiation GO:1904157 ISS
    negative regulation of vascular associated smooth muscle cell differentiation GO:1905064 IDA
    positive regulation of artery morphogenesis GO:1905653 ISS
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified NFATC3 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NFATC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SUFU 51684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 TRAPPC9 83696
Proximity Label-MS Homo sapiens
3 PIP 5304
Affinity Capture-MS Homo sapiens
4 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RP2 6102
Co-fractionation Homo sapiens
6 PABPC3 5042
Affinity Capture-MS Homo sapiens
7 CUL3 8452
Affinity Capture-MS Homo sapiens
8 PPP3R2  
Affinity Capture-MS Homo sapiens
9 SHMT2 6472
Affinity Capture-RNA Homo sapiens
10 TMEM55B 90809
Affinity Capture-MS Homo sapiens
11 SSBP1 6742
Proximity Label-MS Homo sapiens
12 NXF1 10482
Affinity Capture-RNA Homo sapiens
13 APEX1 328
Affinity Capture-RNA Homo sapiens
14 MOV10 4343
Affinity Capture-RNA Homo sapiens
15 ZMIZ1 57178
Affinity Capture-MS Homo sapiens
16 PSMA5 5686
Affinity Capture-MS Homo sapiens
17 MAPK9 5601
Two-hybrid Homo sapiens
18 TRAPPC3 27095
Proximity Label-MS Homo sapiens
19 CDK6 1021
Biochemical Activity Homo sapiens
20 BICD2 23299
Proximity Label-MS Homo sapiens
21 BICD1 636
Proximity Label-MS Homo sapiens
22 ENO1 2023
Affinity Capture-RNA Homo sapiens
23 NCOA3  
Affinity Capture-MS Homo sapiens
24 MAPK8 5599
Two-hybrid Homo sapiens
25 FLOT2 2319
Affinity Capture-MS Homo sapiens
26 FLOT1 10211
Affinity Capture-MS Homo sapiens
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