Gene description for MAPK9
Gene name mitogen-activated protein kinase 9
Gene symbol MAPK9
Other names/aliases JNK-55
JNK2
JNK2A
JNK2ALPHA
JNK2B
JNK2BETA
PRKM9
SAPK
SAPK1a
p54a
p54aSAPK
Species Homo sapiens
 Database cross references - MAPK9
ExoCarta ExoCarta_5601
Vesiclepedia VP_5601
Entrez Gene 5601
HGNC 6886
MIM 602896
UniProt P45984  
 MAPK9 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MAPK9
Molecular Function
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 IEA
    transcription factor binding GO:0008134 IDA
    ATP binding GO:0005524 IEA
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IEA
    protein binding GO:0005515 IPI
    JUN kinase activity GO:0004705 IDA
Biological Process
    JNK cascade GO:0007254 TAS
    rhythmic process GO:0048511 IEA
    protein targeting to mitochondrion GO:0006626 IEA
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    positive regulation of cell morphogenesis involved in differentiation GO:0010770 IEA
    regulation of JNK cascade GO:0046328 IEA
    positive regulation of chemokine production GO:0032722 IEA
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    cellular response to tumor necrosis factor GO:0071356 IEA
    response to drug GO:0042493 IEA
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    cellular response to lipopolysaccharide GO:0071222 IEA
    regulation of protein ubiquitination GO:0031396 IEA
    neuron projection development GO:0031175 IEA
    response to mechanical stimulus GO:0009612 IEA
    positive regulation of prostaglandin biosynthetic process GO:0031394 IEA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    positive regulation of prostaglandin secretion GO:0032308 IEA
    response to stress GO:0006950 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 IEA
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IMP
    cellular response to UV GO:0034644 IEA
    innate immune response GO:0045087 TAS
    regulation of circadian rhythm GO:0042752 ISS
    release of cytochrome c from mitochondria GO:0001836 IEA
    central nervous system development GO:0007417 IEA
    toll-like receptor signaling pathway GO:0002224 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    cellular response to growth factor stimulus GO:0071363 IEA
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    JUN phosphorylation GO:0007258 IEA
    regulation of sequence-specific DNA binding transcription factor activity GO:0051090 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    positive regulation of apoptotic signaling pathway GO:2001235 IEA
    protein phosphorylation GO:0006468 IMP
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    positive regulation of gene expression GO:0010628 IMP
    cellular response to interleukin-1 GO:0071347 IEA
    stress-activated MAPK cascade GO:0051403 TAS
    positive regulation of nitric-oxide synthase biosynthetic process GO:0051770 IEA
    response to amine GO:0014075 IEA
    positive regulation of protein phosphorylation GO:0001934 IEA
    response to cadmium ion GO:0046686 IEA
    response to toxic substance GO:0009636 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IEA
    toll-like receptor 10 signaling pathway GO:0034166 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    mitochondrion GO:0005739 IEA
 Experiment description of studies that identified MAPK9 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAPK9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DUSP19  
Biochemical Activity Homo sapiens
2 DUSP1  
Affinity Capture-Western Homo sapiens
3 TRAF2 7186
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 DUSP10 11221
Affinity Capture-Western Homo sapiens
5 Rps6kb1  
Reconstituted Complex Mus musculus
6 TP53 7157
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Phenotypic Suppression Homo sapiens
7 MAPK8IP2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 ATF7  
Invivo Homo sapiens
Invitro Homo sapiens
9 RB1 5925
Affinity Capture-MS Homo sapiens
10 SF3B4 10262
Two-hybrid Homo sapiens
11 JUND 3727
Invitro Homo sapiens
12 KIAA1712  
Two-hybrid Homo sapiens
13 PRKD1 5587
Affinity Capture-Western Homo sapiens
14 KIAA1377  
Two-hybrid Homo sapiens
15 DUSP16  
Affinity Capture-Western Homo sapiens
16 MAPK8IP1 9479
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 SH3BP5 9467
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
18 ACP5  
Two-hybrid Homo sapiens
19 HSF1  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
20 C19orf14  
Two-hybrid Homo sapiens
21 ZBTB25  
Two-hybrid Homo sapiens
22 MAPK8IP3  
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
23 XPO7 23039
Two-hybrid Homo sapiens
24 XPNPEP1 7511
Two-hybrid Homo sapiens
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