Gene description for PRKD1
Gene name protein kinase D1
Gene symbol PRKD1
Other names/aliases PKC-MU
PKCM
PKD
PRKCM
Species Homo sapiens
 Database cross references - PRKD1
ExoCarta ExoCarta_5587
Vesiclepedia VP_5587
Entrez Gene 5587
HGNC 9407
MIM 605435
UniProt Q15139  
 PRKD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
 Gene ontology annotations for PRKD1
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    protein serine/threonine kinase activity GO:0004674 TAS
    metal ion binding GO:0046872 IEA
    protein kinase C activity GO:0004697 IDA
    ATP binding GO:0005524 IEA
Biological Process
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IGI
    regulation of keratinocyte proliferation GO:0010837 ISS
    intracellular signal transduction GO:0035556 IMP
    positive regulation of blood vessel endothelial cell migration GO:0043536 IMP
    regulation of integrin-mediated signaling pathway GO:2001044 TAS
    angiogenesis GO:0001525 IEA
    protein kinase D signaling GO:0089700 IGI
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    small molecule metabolic process GO:0044281 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    integrin-mediated signaling pathway GO:0007229 TAS
    cellular response to oxidative stress GO:0034599 IDA
    positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway GO:0038033 IMP
    positive regulation of endothelial cell migration GO:0010595 IMP
    signal transduction GO:0007165 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    cell proliferation GO:0008283 TAS
    positive regulation of angiogenesis GO:0045766 IMP
    sphingolipid biosynthetic process GO:0030148 TAS
    Golgi organization GO:0007030 IMP
    sphingolipid metabolic process GO:0006665 TAS
    innate immune response GO:0045087 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IMP
    positive regulation of histone deacetylase activity GO:1901727 IGI
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IMP
    Golgi vesicle transport GO:0048193 ISS
    positive regulation of CREB transcription factor activity GO:0032793 IGI
    protein autophosphorylation GO:0046777 TAS
    positive regulation of neuron projection development GO:0010976 IMP
    positive regulation of endothelial cell proliferation GO:0001938 IGI
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IMP
    negative regulation of cell death GO:0060548 IMP
    apoptotic process GO:0006915 IEA
    negative regulation of endocytosis GO:0045806 TAS
    positive regulation of endothelial cell chemotaxis GO:2001028 IMP
    inflammatory response GO:0006954 IEA
    positive regulation of osteoblast differentiation GO:0045669 ISS
Subcellular Localization
    cytosol GO:0005829 TAS
    cell cortex GO:0005938 IEA
    trans-Golgi network GO:0005802 IDA
    cell-cell junction GO:0005911 IEA
    Golgi apparatus GO:0005794 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IEA
    plasma membrane GO:0005886 IDA
    integral component of plasma membrane GO:0005887 TAS
 Experiment description of studies that identified PRKD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 275
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for PRKD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C1QBP 708
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
2 SYK 6850
Affinity Capture-Western Homo sapiens
3 YWHAQ 10971
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
4 CENTA1  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
5 MT2A 4502
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 BTK 695
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 PLCG1 5335
Affinity Capture-Western Homo sapiens
8 YWHAZ 7534
Reconstituted Complex Homo sapiens
9 PLCG2 5336
Affinity Capture-Western Homo sapiens
10 MAPK8 5599
Affinity Capture-Western Homo sapiens
11 HDAC5 10014
Affinity Capture-Western Homo sapiens
12 MAPK9 5601
Affinity Capture-Western Homo sapiens
13 JUN 3725
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which PRKD1 is involved
PathwayEvidenceSource
Sphingolipid de novo biosynthesis TAS Reactome





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