Gene description for PRKD1
Gene name protein kinase D1
Gene symbol PRKD1
Other names/aliases PKC-MU
PKCM
PKD
PRKCM
Species Homo sapiens
 Database cross references - PRKD1
ExoCarta ExoCarta_5587
Vesiclepedia VP_5587
Entrez Gene 5587
HGNC 9407
MIM 605435
UniProt Q15139  
 PRKD1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
 Gene ontology annotations for PRKD1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 IMP
    protein serine/threonine kinase activity GO:0004674 TAS
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 IDA
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 TAS
    calcium,diacylglycerol-dependent serine/threonine kinase activity GO:0004698 IEA
    diacylglycerol-dependent, calcium-independent serine/threonine kinase activity GO:0004699 IEA
    protein kinase C binding GO:0005080 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    kinase activity GO:0016301 IDA
    heat shock protein binding GO:0031072 IEA
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    protein serine kinase activity GO:0106310 IGI
    phosphatidylinositol 3-kinase activator activity GO:0141038 IDA
Biological Process
    angiogenesis GO:0001525 IEA
    positive regulation of endothelial cell proliferation GO:0001938 IGI
    apoptotic process GO:0006915 IEA
    inflammatory response GO:0006954 IEA
    Golgi organization GO:0007030 IMP
    signal transduction GO:0007165 TAS
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IBA
    integrin-mediated signaling pathway GO:0007229 TAS
    nervous system development GO:0007399 IEA
    positive regulation of autophagy GO:0010508 IMP
    positive regulation of endothelial cell migration GO:0010595 IMP
    positive regulation of gene expression GO:0010628 IEA
    regulation of keratinocyte proliferation GO:0010837 ISS
    positive regulation of neuron projection development GO:0010976 IMP
    regulation of skeletal muscle contraction by modulation of calcium ion sensitivity of myofibril GO:0014723 IEA
    peptidyl-serine phosphorylation GO:0018105 IMP
    peptidyl-threonine phosphorylation GO:0018107 IMP
    peptidyl-threonine phosphorylation GO:0018107 ISS
    sphingolipid biosynthetic process GO:0030148 TAS
    cell differentiation GO:0030154 IEA
    cellular response to amino acid starvation GO:0034198 IMP
    cellular response to oxidative stress GO:0034599 IDA
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IMP
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IGI
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IMP
    positive regulation of protein import into nucleus GO:0042307 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    positive regulation of blood vessel endothelial cell migration GO:0043536 IGI
    positive regulation of blood vessel endothelial cell migration GO:0043536 IMP
    innate immune response GO:0045087 IEA
    positive regulation of osteoblast differentiation GO:0045669 ISS
    positive regulation of angiogenesis GO:0045766 IGI
    positive regulation of angiogenesis GO:0045766 IMP
    positive regulation of cell size GO:0045793 IEA
    negative regulation of endocytosis GO:0045806 TAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    protein autophosphorylation GO:0046777 IDA
    protein autophosphorylation GO:0046777 TAS
    positive regulation of protein export from nucleus GO:0046827 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IGI
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IMP
    Golgi vesicle transport GO:0048193 ISS
    defense response to Gram-negative bacterium GO:0050829 IEA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    positive regulation of sarcomere organization GO:0060298 IEA
    cellular response to hydroperoxide GO:0071447 IMP
    cellular response to norepinephrine stimulus GO:0071874 IEA
    positive regulation of peptide hormone secretion GO:0090277 IEA
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 ISS
    cellular response to angiotensin GO:1904385 IEA
    cellular response to phorbol 13-acetate 12-myristate GO:1904628 IEA
    cellular response to endothelin GO:1990859 IEA
    positive regulation of endothelial cell chemotaxis GO:2001028 IGI
    positive regulation of endothelial cell chemotaxis GO:2001028 IMP
    regulation of integrin-mediated signaling pathway GO:2001044 TAS
Subcellular Localization
    autophagosome membrane GO:0000421 IDA
    nucleus GO:0005634 IEA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    cell-cell junction GO:0005911 IEA
    cell cortex GO:0005938 IEA
    Z disc GO:0030018 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified PRKD1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for PRKD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BTK 695
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 DISC1 27185
Affinity Capture-MS Homo sapiens
3 GAN 8139
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 PER2  
Proximity Label-MS Homo sapiens
6 PRKD3 23683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HOXD11  
Affinity Capture-MS Homo sapiens
8 COPS2 9318
Biochemical Activity Homo sapiens
9 COPS7A 50813
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
10 BAD  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 TP53 7157
Biochemical Activity Homo sapiens
12 AKAP13 11214
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TFAP2A  
Biochemical Activity Homo sapiens
15 YWHAE 7531
Affinity Capture-MS Homo sapiens
16 ADAP1  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
17 ZNF777  
Affinity Capture-MS Homo sapiens
18 MAPK8 5599
Affinity Capture-Western Homo sapiens
19 YWHAZ 7534
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
20 GRIPAP1 56850
Affinity Capture-MS Homo sapiens
21 PRKD1 5587
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
22 HSPB1 3315
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
23 FUCA1 2517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
25 PKMYT1  
Affinity Capture-MS Homo sapiens
26 KRT27 342574
Affinity Capture-MS Homo sapiens
27 MT2A 4502
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
28 PPP1R14A 94274
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
29 HDAC7  
Affinity Capture-Western Homo sapiens
30 PLCG2 5336
Affinity Capture-Western Homo sapiens
31 JUN 3725
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
32 YAP1 10413
Affinity Capture-MS Homo sapiens
33 MAPK9 5601
Affinity Capture-Western Homo sapiens
34 IBTK 25998
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
35 PRKD2 25865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 KLHDC10 23008
Affinity Capture-MS Homo sapiens
37 LPIN2  
Affinity Capture-MS Homo sapiens
38 COPS5 10987
Biochemical Activity Homo sapiens
39 C1QBP 708
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
40 YWHAQ 10971
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
41 Mapk13  
Affinity Capture-MS Mus musculus
42 AR 367
Affinity Capture-MS Homo sapiens
43 SYK 6850
Affinity Capture-Western Homo sapiens
44 TJP2 9414
Affinity Capture-MS Homo sapiens
45 PLCG1 5335
Affinity Capture-Western Homo sapiens
46 SPRY2 10253
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
47 HDAC5 10014
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
48 USP28 57646
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
49 NOS1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
50 SIX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PRKD1 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Sphingolipid de novo biosynthesis TAS Reactome
Sphingolipid metabolism TAS Reactome





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