Gene description for AKAP13
Gene name A kinase (PRKA) anchor protein 13
Gene symbol AKAP13
Other names/aliases AKAP-13
AKAP-Lbc
ARHGEF13
BRX
HA-3
Ht31
LBC
PRKA13
PROTO-LB
PROTO-LBC
c-lbc
p47
Species Homo sapiens
 Database cross references - AKAP13
ExoCarta ExoCarta_11214
Vesiclepedia VP_11214
Entrez Gene 11214
HGNC 371
MIM 604686
UniProt Q12802  
 AKAP13 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for AKAP13
Molecular Function
    protein complex scaffold GO:0032947 IEA
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    Rho GTPase binding GO:0017048 IEA
    cAMP-dependent protein kinase activity GO:0004691 NAS
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 TAS
    metal ion binding GO:0046872 IEA
    protein kinase A binding GO:0051018 IEA
Biological Process
    protein phosphorylation GO:0006468 NAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    regulation of glucocorticoid mediated signaling pathway GO:1900169 IEA
    positive regulation of apoptotic process GO:0043065 TAS
    positive regulation of GTPase activity GO:0043547 IEA
    apoptotic signaling pathway GO:0097190 TAS
    regulation of cardiac muscle hypertrophy GO:0010611 IEA
    regulation of protein kinase activity GO:0045859 IEA
    nuclear export GO:0051168 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    membrane GO:0016020 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified AKAP13 in exosomes
1
Experiment ID 234
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AKAP13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ESR1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
2 PRKAR2A 5576
Protein-peptide Homo sapiens
Invitro Homo sapiens
3 BAK1 578
Affinity Capture-MS Homo sapiens
4 GNA12 2768
Affinity Capture-Western Homo sapiens
5 GNAQ 2776
Affinity Capture-Western Homo sapiens
6 RHOA 387
Affinity Capture-Western Homo sapiens
7 PPARA  
Reconstituted Complex Homo sapiens
8 THRA  
Reconstituted Complex Homo sapiens
9 RXRB  
Reconstituted Complex Homo sapiens
10 YWHAB 7529
Affinity Capture-MS Homo sapiens
11 CTNNAL1 8727
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which AKAP13 is involved
PathwayEvidenceSource
G alpha (12/13) signalling events TAS Reactome
NRAGE signals death through JNK TAS Reactome
Rho GTPase cycle TAS Reactome





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