Gene description for ARHGEF18
Gene name Rho/Rac guanine nucleotide exchange factor (GEF) 18
Gene symbol ARHGEF18
Other names/aliases P114-RhoGEF
Species Homo sapiens
 Database cross references - ARHGEF18
ExoCarta ExoCarta_23370
Entrez Gene 23370
HGNC 17090
UniProt Q6ZSZ5  
 ARHGEF18 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ARHGEF18
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
Biological Process
    positive regulation of apoptotic process GO:0043065 TAS
    positive regulation of GTPase activity GO:0043547 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    regulation of cell shape GO:0008360 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    actin cytoskeleton organization GO:0030036 IDA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    apoptotic signaling pathway GO:0097190 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    cell junction GO:0030054 TAS
    cytosol GO:0005829 TAS
    apical part of cell GO:0045177 IDA
 Experiment description of studies that identified ARHGEF18 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ARHGEF18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RHOA 387
Reconstituted Complex Homo sapiens
2 GNB1 2782
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here