Gene description for BAK1
Gene name BCL2-antagonist/killer 1
Gene symbol BAK1
Other names/aliases BAK
BAK-LIKE
BCL2L7
CDN1
Species Homo sapiens
 Database cross references - BAK1
ExoCarta ExoCarta_578
Vesiclepedia VP_578
Entrez Gene 578
HGNC 949
MIM 600516
UniProt Q16611  
 BAK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for BAK1
Molecular Function
    BH domain binding GO:0051400 IEA
    chaperone binding GO:0051087 IEA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IBA
    protein heterodimerization activity GO:0046982 IPI
    metal ion binding GO:0046872 IEA
    heat shock protein binding GO:0031072 IEA
Biological Process
    myeloid cell homeostasis GO:0002262 IEA
    activation of signaling protein activity involved in unfolded protein response GO:0006987 TAS
    B cell apoptotic process GO:0001783 IEA
    establishment or maintenance of transmembrane electrochemical gradient GO:0010248 IDA
    negative regulation of gene expression GO:0010629 IEA
    positive regulation of proteolysis GO:0045862 IDA
    aging GO:0007568 IEA
    cellular response to UV GO:0034644 ISS
    positive regulation of apoptotic process GO:0043065 IMP
    fibroblast apoptotic process GO:0044346 IEA
    response to organic cyclic compound GO:0014070 IEA
    response to fungus GO:0009620 IEA
    endoplasmic reticulum calcium ion homeostasis GO:0032469 TAS
    response to hydrogen peroxide GO:0042542 IEA
    regulation of mitochondrial membrane permeability GO:0046902 IDA
    regulation of mitochondrial membrane potential GO:0051881 IDA
    negative regulation of cell proliferation GO:0008285 IEA
    response to drug GO:0042493 IEA
    organ regeneration GO:0031100 IEA
    programmed cell death GO:0012501 TAS
    positive regulation of release of cytochrome c from mitochondria GO:0090200 IEA
    regulation of protein homodimerization activity GO:0043496 IDA
    blood vessel remodeling GO:0001974 IEA
    limb morphogenesis GO:0035108 IEA
    negative regulation of endoplasmic reticulum calcium ion concentration GO:0032471 IEA
    response to UV-C GO:0010225 IEA
    mitochondrial fusion GO:0008053 IEA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 TAS
    negative regulation of peptidyl-serine phosphorylation GO:0033137 IEA
    thymocyte apoptotic process GO:0070242 IEA
    regulation of cell cycle GO:0051726 IEA
    B cell homeostasis GO:0001782 IEA
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IBA
    cellular response to mechanical stimulus GO:0071260 IEP
    apoptotic signaling pathway GO:0097190 IMP
    positive regulation of endoplasmic reticulum unfolded protein response GO:1900103 IMP
    activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c GO:0008635 IEA
    response to ethanol GO:0045471 IEA
    apoptotic process involved in patterning of blood vessels GO:1902262 IEA
    response to mycotoxin GO:0010046 IEA
    apoptotic process GO:0006915 TAS
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IBA
    activation of cysteine-type endopeptidase activity GO:0097202 IDA
    endocrine pancreas development GO:0031018 IEA
    brain development GO:0007420 IEA
    post-embryonic camera-type eye morphogenesis GO:0048597 IEA
    B cell negative selection GO:0002352 IEA
    release of cytochrome c from mitochondria GO:0001836 IGI
    cell proliferation GO:0008283 IEA
    cytolysis GO:0019835 IMP
    positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901030 TAS
    vagina development GO:0060068 IEA
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    positive regulation of IRE1-mediated unfolded protein response GO:1903896 TAS
    response to gamma radiation GO:0010332 IEA
    positive regulation of calcium ion transport into cytosol GO:0010524 IEA
    regulation of protein heterodimerization activity GO:0043497 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    mitochondrial outer membrane GO:0005741 TAS
    integral component of mitochondrial outer membrane GO:0031307 ISS
    mitochondrion GO:0005739 IDA
    pore complex GO:0046930 IDA
    cytosol GO:0005829 IEA
 Experiment description of studies that identified BAK1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BAK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FRYL 285527
Affinity Capture-MS Homo sapiens
2 DFFA 1676
Affinity Capture-MS Homo sapiens
3 MCL1 4170
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 BCL2L1 598
Protein-peptide Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 BAK1 578
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 SRRM2 23524
Affinity Capture-MS Homo sapiens
7 FBL 2091
Affinity Capture-MS Homo sapiens
8 HSPD1 3329
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
9 BCL2  
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
10 BCL2A1  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
11 BAX 581
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
12 KRT31 3881
Affinity Capture-MS Homo sapiens
13 MYCBP2 23077
Affinity Capture-MS Homo sapiens
14 VDAC2 7417
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 AKAP13 11214
Affinity Capture-MS Homo sapiens
17 ACTR2 10097
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which BAK1 is involved
PathwayEvidenceSource
Activation and oligomerization of BAK protein TAS Reactome





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