Gene description for BAK1
Gene name BCL2-antagonist/killer 1
Gene symbol BAK1
Other names/aliases BAK
BAK-LIKE
BCL2L7
CDN1
Species Homo sapiens
 Database cross references - BAK1
ExoCarta ExoCarta_578
Vesiclepedia VP_578
Entrez Gene 578
HGNC 949
MIM 600516
UniProt Q16611  
 BAK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for BAK1
Molecular Function
    protein binding GO:0005515 IPI
    channel activity GO:0015267 IBA
    porin activity GO:0015288 IDA
    heat shock protein binding GO:0031072 IEA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    transmembrane transporter binding GO:0044325 IPI
    protein-containing complex binding GO:0044877 IEA
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 IPI
    protein-folding chaperone binding GO:0051087 IEA
    BH domain binding GO:0051400 IEA
Biological Process
    B cell homeostasis GO:0001782 IEA
    B cell apoptotic process GO:0001783 IEA
    release of cytochrome c from mitochondria GO:0001836 IBA
    release of cytochrome c from mitochondria GO:0001836 IDA
    release of cytochrome c from mitochondria GO:0001836 IGI
    blood vessel remodeling GO:0001974 IEA
    myeloid cell homeostasis GO:0002262 IEA
    B cell negative selection GO:0002352 IEA
    apoptotic process GO:0006915 IDA
    apoptotic process GO:0006915 NAS
    mitochondrial fusion GO:0008053 IEA
    negative regulation of cell population proliferation GO:0008285 IEA
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IBA
    response to xenobiotic stimulus GO:0009410 IEA
    response to fungus GO:0009620 IEA
    response to mycotoxin GO:0010046 IEA
    response to UV-C GO:0010225 IEA
    establishment or maintenance of transmembrane electrochemical gradient GO:0010248 IDA
    response to gamma radiation GO:0010332 IEA
    positive regulation of calcium ion transport into cytosol GO:0010524 IEA
    negative regulation of gene expression GO:0010629 IEA
    response to organic cyclic compound GO:0014070 IEA
    endocrine pancreas development GO:0031018 IEA
    animal organ regeneration GO:0031100 IEA
    positive regulation of protein-containing complex assembly GO:0031334 IDA
    endoplasmic reticulum calcium ion homeostasis GO:0032469 TAS
    negative regulation of endoplasmic reticulum calcium ion concentration GO:0032471 IEA
    cellular response to unfolded protein GO:0034620 TAS
    cellular response to UV GO:0034644 IMP
    cellular response to UV GO:0034644 ISS
    limb morphogenesis GO:0035108 IEA
    response to hydrogen peroxide GO:0042542 IEA
    positive regulation of apoptotic process GO:0043065 IBA
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of apoptotic process GO:0043065 ISS
    fibroblast apoptotic process GO:0044346 IEA
    response to ethanol GO:0045471 IEA
    positive regulation of proteolysis GO:0045862 IDA
    regulation of mitochondrial membrane permeability GO:0046902 IDA
    post-embryonic camera-type eye morphogenesis GO:0048597 IEA
    epithelial cell proliferation GO:0050673 IEA
    establishment of localization in cell GO:0051649 IEA
    regulation of cell cycle GO:0051726 IEA
    regulation of mitochondrial membrane potential GO:0051881 IDA
    vagina development GO:0060068 IEA
    calcium ion transport into cytosol GO:0060402 IEA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 TAS
    thymocyte apoptotic process GO:0070242 IEA
    cellular response to mechanical stimulus GO:0071260 IEP
    positive regulation of release of cytochrome c from mitochondria GO:0090200 NAS
    negative regulation of release of cytochrome c from mitochondria GO:0090201 IDA
    apoptotic signaling pathway GO:0097190 IMP
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IBA
    intrinsic apoptotic signaling pathway GO:0097193 IDA
    activation of cysteine-type endopeptidase activity GO:0097202 IDA
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 IDA
    positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901030 NAS
    apoptotic process involved in blood vessel morphogenesis GO:1902262 IEA
    positive regulation of IRE1-mediated unfolded protein response GO:1903896 TAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IBA
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 ISS
    mitochondrial outer membrane GO:0005741 TAS
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 IEA
    pore complex GO:0046930 IDA
    Bcl-2 family protein complex GO:0097136 IDA
    Bcl-2 family protein complex GO:0097136 IPI
    BAK complex GO:0097145 IDA
    BAK complex GO:0097145 IPI
 Experiment description of studies that identified BAK1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BAK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HERC1 8925
Protein-peptide Homo sapiens
Co-localization Homo sapiens
2 TOMM7 54543
Co-fractionation Homo sapiens
3 ACTR2 10097
Affinity Capture-MS Homo sapiens
4 BCL2A1  
Two-hybrid Homo sapiens
5 HSD17B4 3295
Two-hybrid Homo sapiens
6 FASN 2194
Positive Genetic Homo sapiens
7 TOMM40 10452
Co-fractionation Homo sapiens
8 BAX 581
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
9 MYCBP2 23077
Affinity Capture-MS Homo sapiens
10 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 A2ML1 144568
Affinity Capture-MS Homo sapiens
12 FRYL 285527
Affinity Capture-MS Homo sapiens
13 MCL1 4170
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 DFFA 1676
Affinity Capture-MS Homo sapiens
15 DBN1 1627
Two-hybrid Homo sapiens
16 BID  
Protein-peptide Homo sapiens
17 AKAP13 11214
Affinity Capture-MS Homo sapiens
18 ARHGDIA 396
Two-hybrid Homo sapiens
19 AMY1C 278
Affinity Capture-MS Homo sapiens
20 KIAA1429 25962
Affinity Capture-MS Homo sapiens
21 BAK1 578
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 BCL2L1 598
Protein-peptide Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
23 BCL2L2 599
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
24 BCL2  
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 SRRM2 23524
Affinity Capture-MS Homo sapiens
26 FBL 2091
Affinity Capture-MS Homo sapiens
27 NFKBIB  
Biochemical Activity Homo sapiens
28 HSPD1 3329
Affinity Capture-Western Homo sapiens
29 ABCB10 23456
Co-fractionation Homo sapiens
30 MAPK8 5599
Affinity Capture-Western Homo sapiens
31 KRT31 3881
Affinity Capture-MS Homo sapiens
32 VDAC2 7417
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
33 GIMAP5  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here