Gene description for Prkacb
Gene name protein kinase, cAMP dependent, catalytic, beta
Gene symbol Prkacb
Other names/aliases Pkacb
Species Mus musculus
 Database cross references - Prkacb
ExoCarta ExoCarta_18749
Vesiclepedia VP_18749
Entrez Gene 18749
UniProt P68181  
 Prkacb identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prkacb
Molecular Function
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    ATP binding GO:0005524 ISO
    magnesium ion binding GO:0000287 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    protein kinase activity GO:0004672 IEA
    nucleotide binding GO:0000166 IEA
    transferase activity GO:0016740 IEA
    cAMP-dependent protein kinase activity GO:0004691 ISO
    kinase activity GO:0016301 IEA
    protein serine/threonine kinase activity GO:0004674 IEA
Biological Process
    regulation of protein processing GO:0070613 IGI
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 TAS
    phosphorylation GO:0016310 IEA
    negative regulation of meiotic cell cycle GO:0051447 ISO
    neural tube closure GO:0001843 IGI
    protein phosphorylation GO:0006468 ISO
    negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning GO:1901621 IGI
Subcellular Localization
    ciliary base GO:0097546 IDA
    plasma membrane GO:0005886 IEA
    cytoplasm GO:0005737 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    cAMP-dependent protein kinase complex GO:0005952 TAS
    cytosol GO:0005829 ISO
    membrane GO:0016020 IEA
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    centrosome GO:0005813 ISO
 Experiment description of studies that identified Prkacb in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prkacb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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