Gene description for PRKAR1A
Gene name protein kinase, cAMP-dependent, regulatory, type I, alpha
Gene symbol PRKAR1A
Other names/aliases ACRDYS1
ADOHR
CAR
CNC
CNC1
PKR1
PPNAD1
PRKAR1
TSE1
Species Homo sapiens
 Database cross references - PRKAR1A
ExoCarta ExoCarta_5573
Entrez Gene 5573
HGNC 9388
MIM 188830
UniProt P10644  
 PRKAR1A identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for PRKAR1A
Molecular Function
    cAMP binding GO:0030552 IEA
    protein kinase A catalytic subunit binding GO:0034236 IPI
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IDA
    ubiquitin protein ligase binding GO:0031625 IDA
    cAMP-dependent protein kinase regulator activity GO:0008603 IDA
    protein binding GO:0005515 IPI
Biological Process
    small molecule metabolic process GO:0044281 TAS
    transmembrane transport GO:0055085 TAS
    blood coagulation GO:0007596 TAS
    energy reserve metabolic process GO:0006112 TAS
    negative regulation of cAMP-dependent protein kinase activity GO:2000480 IDA
    innate immune response GO:0045087 TAS
    activation of phospholipase C activity GO:0007202 TAS
    cellular response to glucagon stimulus GO:0071377 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    mesoderm formation GO:0001707 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    female meiotic division GO:0007143 IEA
    regulation of insulin secretion GO:0050796 TAS
    negative regulation of meiotic nuclear division GO:0045835 IEA
    water transport GO:0006833 TAS
    renal water homeostasis GO:0003091 TAS
    sarcomere organization GO:0045214 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    activation of protein kinase A activity GO:0034199 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    intracellular signal transduction GO:0035556 TAS
    signal transduction GO:0007165 TAS
    cardiac muscle cell proliferation GO:0060038 IEA
Subcellular Localization
    neuromuscular junction GO:0031594 IEA
    cAMP-dependent protein kinase complex GO:0005952 IEA
    AMP-activated protein kinase complex GO:0031588 IDA
    plasma membrane GO:0005886 IEA
    protein complex GO:0043234 IDA
    membrane GO:0016020 IDA
    ciliary base GO:0097546 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PRKAR1A in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRKAR1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2M 9040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 MCRS1  
Affinity Capture-MS Homo sapiens
3 AKAP11 11215
Invivo Homo sapiens
4 PRKX 5613
Invivo Homo sapiens
Invitro Homo sapiens
5 PRKAR1A 5573
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 PRKACA 5566
Affinity Capture-MS Homo sapiens
7 ARFGEF2 10564
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 PLEKHF2 79666
Two-hybrid Homo sapiens
9 ARK5  
Affinity Capture-MS Homo sapiens
10 ARFGEF1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 PRKAR1B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 TUSC4  
Affinity Capture-MS Homo sapiens
13 EGFR 1956
Affinity Capture-Western Homo sapiens
14 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
15 AKAP1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
16 C2orf88 84281
Two-hybrid Homo sapiens
17 PRPF40A 55660
Affinity Capture-MS Homo sapiens
18 PYCARD  
Two-hybrid Homo sapiens
19 MYO7A 4647
Invivo Homo sapiens
Two-hybrid Homo sapiens
20 AKAP4  
Two-hybrid Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
21 AKAP10  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
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