Gene description for MYO7A
Gene name myosin VIIA
Gene symbol MYO7A
Other names/aliases DFNA11
DFNB2
MYOVIIA
MYU7A
NSRD2
USH1B
Species Homo sapiens
 Database cross references - MYO7A
ExoCarta ExoCarta_4647
Entrez Gene 4647
HGNC 7606
MIM 276903
UniProt Q13402  
 MYO7A identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocytes 26054723    
 Gene ontology annotations for MYO7A
Molecular Function
    actin filament binding GO:0051015 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    calmodulin binding GO:0005516 IMP
    actin-dependent ATPase activity GO:0030898 IEA
    microfilament motor activity GO:0000146 IDA
    protein homodimerization activity GO:0042803 IEA
    spectrin binding GO:0030507 IDA
    ADP binding GO:0043531 IEA
    protein domain specific binding GO:0019904 IEA
Biological Process
    sensory perception of light stimulus GO:0050953 IMP
    lysosome organization GO:0007040 IDA
    metabolic process GO:0008152 IEA
    post-embryonic organ morphogenesis GO:0048563 IEA
    visual perception GO:0007601 IMP
    actin filament-based movement GO:0030048 IDA
    pigment granule transport GO:0051904 IEA
    equilibrioception GO:0050957 IMP
    auditory receptor cell stereocilium organization GO:0060088 IEA
    intracellular protein transport GO:0006886 IEA
    sensory perception of sound GO:0007605 IMP
    phagolysosome assembly GO:0001845 IEA
    eye photoreceptor cell development GO:0042462 IC
Subcellular Localization
    cytoplasm GO:0005737 IDA
    apical plasma membrane GO:0016324 IEA
    melanosome GO:0042470 IEA
    lysosomal membrane GO:0005765 IDA
    cell cortex GO:0005938 IEA
    photoreceptor connecting cilium GO:0032391 IEA
    photoreceptor inner segment GO:0001917 IDA
    stereocilium GO:0032420 IEA
    photoreceptor outer segment GO:0001750 IDA
    cytosol GO:0005829 IDA
    myosin VII complex GO:0031477 IEA
    synapse GO:0045202 IDA
 Experiment description of studies that identified MYO7A in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MYO7A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 USH1C  
Reconstituted Complex Homo sapiens
2 CTNNB1 1499
Affinity Capture-Western Homo sapiens
3 KEAP1 9817
Two-hybrid Homo sapiens
4 PRKAR1A 5573
Invivo Homo sapiens
Two-hybrid Homo sapiens
5 CTNNA1 1495
Affinity Capture-Western Homo sapiens
6 CDH1 999
Affinity Capture-Western Homo sapiens
7 MAP2 4133
Invivo Homo sapiens
8 MYRIP  
Invitro Homo sapiens
9 VEZATIN  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which MYO7A is involved
PathwayEvidenceSource
The canonical retinoid cycle in rods (twilight vision) TAS Reactome





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