Experiment description of studies that identified MAP2 in sEVs

1 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 193 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ PHB Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 194 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of basement membrane nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 195 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of early IgA nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for MAP2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PA2G4 5036 Co-fractionation Homo sapiens 2 MARK3 4140 Biochemical Activity Homo sapiens 3 YBX1 4904 Co-fractionation Homo sapiens 4 MARK4   Biochemical Activity Homo sapiens 5 ACTN1 87 Reconstituted Complex Homo sapiens 6 NTRK1 4914 Affinity Capture-MS Homo sapiens 7 MYCN   Affinity Capture-MS Homo sapiens 8 NMT1 4836 Co-fractionation Homo sapiens 9 NEDD4 4734 Affinity Capture-Western Homo sapiens 10 GRB2 2885 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 11 PLEC 5339 Reconstituted Complex Homo sapiens 12 EIF3C 8663 Co-fractionation Homo sapiens 13 MAP2 4133 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 14 EIF3K 27335 Co-fractionation Homo sapiens 15 DAG1 1605 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 16 GPM6A 2823 Affinity Capture-MS Homo sapiens 17 DYNC1I2 1781 Co-fractionation Homo sapiens 18 PSMD8 5714 Co-fractionation Homo sapiens 19 ABCF1 23 Co-fractionation Homo sapiens 20 PRKACG   Affinity Capture-MS Homo sapiens 21 HNRNPA0 10949 Co-fractionation Homo sapiens 22 MYO7A 4647 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 23 MECP2 4204 Affinity Capture-MS Homo sapiens 24 MAPRE3   Proximity Label-MS Homo sapiens 25 NUFIP1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 MSX2   Affinity Capture-MS Homo sapiens 27 PRKAR2B 5577 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 APP 351 Reconstituted Complex Homo sapiens 29 TUBA1A 7846 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 30 MAPRE1 22919 Proximity Label-MS Homo sapiens 31 GSK3A 2931 Affinity Capture-MS Homo sapiens 32 DIMT1 27292 Co-fractionation Homo sapiens 33 PRKACA 5566 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 PARK2   Affinity Capture-Western Homo sapiens 35 ARMC1 55156 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 MAPT   Reconstituted Complex Homo sapiens Co-fractionation Homo sapiens 37 IGF2BP3 10643 Co-fractionation Homo sapiens 38 NEFL 4747 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 39 FYN 2534 Affinity Capture-Western Homo sapiens 40 HECW2   Affinity Capture-MS Homo sapiens 41 Nedd4 25489 Biochemical Activity Rattus norvegicus Reconstituted Complex Rattus norvegicus 42 PSMC5 5705 Co-fractionation Homo sapiens 43 DGUOK   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 NEDD4L 23327 Reconstituted Complex Homo sapiens 45 DCTN1 1639 Proximity Label-MS Homo sapiens 46 JUNB   Reconstituted Complex Homo sapiens 47 PRKACB 5567 Affinity Capture-MS Homo sapiens 48 RPL23A 6147 Co-fractionation Homo sapiens 49 SRC 6714 Reconstituted Complex Homo sapiens 50 DHX9 1660 Co-fractionation Homo sapiens 51 FBXW8 26259 Affinity Capture-MS Homo sapiens 52 HAX1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 53 OBSL1 23363 Affinity Capture-MS Homo sapiens 54 PRKAR2A 5576 Affinity Capture-MS Homo sapiens 55 MDM2   Biochemical Activity Homo sapiens 56 RAE1 8480 Co-fractionation Homo sapiens 57 PSMD13 5719 Co-fractionation Homo sapiens View the network

Pathways in which MAP2 is involved

No pathways found