Gene description for DIMT1
Gene name DIM1 dimethyladenosine transferase 1 homolog (S. cerevisiae)
Gene symbol DIMT1
Other names/aliases DIM1
DIMT1L
HSA9761
HUSSY5
Species Homo sapiens
 Database cross references - DIMT1
ExoCarta ExoCarta_27292
Vesiclepedia VP_27292
Entrez Gene 27292
HGNC 30217
MIM 612499
UniProt Q9UNQ2  
 DIMT1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for DIMT1
Molecular Function
    rRNA (adenine-N6,N6-)-dimethyltransferase activity GO:0000179 EXP
    rRNA (adenine-N6,N6-)-dimethyltransferase activity GO:0000179 IBA
    RNA binding GO:0003723 HDA
    18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity GO:0052909 IMP
Biological Process
    rRNA methylation GO:0031167 IBA
    rRNA methylation GO:0031167 IMP
    rRNA methylation GO:0031167 TAS
    ribosomal small subunit biogenesis GO:0042274 IDA
    positive regulation of rRNA processing GO:2000234 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    small-subunit processome GO:0032040 IDA
 Experiment description of studies that identified DIMT1 in exosomes
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DIMT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RPS12 6206
Affinity Capture-MS Homo sapiens
3 SPRTN  
Affinity Capture-MS Homo sapiens
4 PDAP1 11333
Affinity Capture-MS Homo sapiens
5 EIF3K 27335
Co-fractionation Homo sapiens
6 TARDBP 23435
Affinity Capture-MS Homo sapiens
7 EIF2B2 8892
Co-fractionation Homo sapiens
8 TRIM66  
Affinity Capture-MS Homo sapiens
9 ABCF1 23
Co-fractionation Homo sapiens
10 RPS19 6223
Affinity Capture-MS Homo sapiens
11 RPL10 6134
Affinity Capture-MS Homo sapiens
12 BYSL 705
Affinity Capture-MS Homo sapiens
13 CAND1 55832
Affinity Capture-MS Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 APP 351
Reconstituted Complex Homo sapiens
16 ILF2 3608
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
17 USP36  
Affinity Capture-MS Homo sapiens
18 RPL10A 4736
Affinity Capture-MS Homo sapiens
19 IGF2BP3 10643
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
20 KIF23 9493
Affinity Capture-MS Homo sapiens
21 H1FX 8971
Affinity Capture-MS Homo sapiens
22 AP2A1 160
Affinity Capture-MS Homo sapiens
23 FBXW7  
Affinity Capture-MS Homo sapiens
24 RPL24 6152
Affinity Capture-MS Homo sapiens
25 NPM1 4869
Affinity Capture-MS Homo sapiens
26 THTPA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SRP14 6727
Affinity Capture-MS Homo sapiens
28 FAM207A  
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
29 GRWD1 83743
Co-fractionation Homo sapiens
30 ITGA4 3676
Affinity Capture-MS Homo sapiens
31 SRP68 6730
Affinity Capture-MS Homo sapiens
32 VCAM1 7412
Affinity Capture-MS Homo sapiens
33 MYCN  
Affinity Capture-MS Homo sapiens
34 RBM42  
Affinity Capture-MS Homo sapiens
35 RPS11 6205
Co-fractionation Homo sapiens
36 RBM8A 9939
Affinity Capture-MS Homo sapiens
37 RPS26 6231
Affinity Capture-MS Homo sapiens
38 MAP2 4133
Co-fractionation Homo sapiens
39 LIN28A  
Affinity Capture-MS Homo sapiens
40 EDC4 23644
Co-fractionation Homo sapiens
41 SRP9 6726
Affinity Capture-MS Homo sapiens
42 FOXA1  
Affinity Capture-MS Homo sapiens
43 UTP14A 10813
Affinity Capture-MS Homo sapiens
44 RPL26 6154
Affinity Capture-MS Homo sapiens
45 COPE 11316
Affinity Capture-MS Homo sapiens
46 DRG1 4733
Affinity Capture-MS Homo sapiens
47 STAU1 6780
Affinity Capture-MS Homo sapiens
48 RPL5 6125
Affinity Capture-MS Homo sapiens
49 RPL31 6160
Affinity Capture-MS Homo sapiens
50 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
51 NELFB 25920
Co-fractionation Homo sapiens
52 TRIM11  
Affinity Capture-MS Homo sapiens
53 MTPAP 55149
Affinity Capture-MS Homo sapiens
54 RFC4 5984
Co-fractionation Homo sapiens
55 DDX1 1653
Affinity Capture-MS Homo sapiens
56 DHX9 1660
Affinity Capture-MS Homo sapiens
57 HECTD1 25831
Affinity Capture-MS Homo sapiens
58 MAGEB2 4113
Affinity Capture-MS Homo sapiens
59 DDX39B 7919
Affinity Capture-MS Homo sapiens
60 RPS15A 6210
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
61 CEBPA  
Protein-peptide Homo sapiens
62 CYLD  
Affinity Capture-MS Homo sapiens
63 BAG3 9531
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
64 ANLN 54443
Affinity Capture-MS Homo sapiens
65 ZBTB2 57621
Affinity Capture-MS Homo sapiens
66 USP42  
Affinity Capture-MS Homo sapiens
67 DNAJC21  
Affinity Capture-MS Homo sapiens
68 RPL23 9349
Affinity Capture-MS Homo sapiens
69 RPS2 6187
Affinity Capture-MS Homo sapiens
70 PTPN6 5777
Affinity Capture-MS Homo sapiens
71 CTNNB1 1499
Affinity Capture-MS Homo sapiens
72 BRD8 10902
Affinity Capture-MS Homo sapiens
73 CUL3 8452
Affinity Capture-MS Homo sapiens
74 RPL21 6144
Affinity Capture-MS Homo sapiens
75 RPL3 6122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
77 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
78 EIF2B3 8891
Co-fractionation Homo sapiens
79 RPL7 6129
Affinity Capture-MS Homo sapiens
80 GPRASP2  
Affinity Capture-MS Homo sapiens
81 FN1 2335
Affinity Capture-MS Homo sapiens
82 AP2M1 1173
Affinity Capture-MS Homo sapiens
83 MAP2K3 5606
Affinity Capture-MS Homo sapiens
84 PCBP1 5093
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 NMT1 4836
Co-fractionation Homo sapiens
86 DKC1 1736
Co-fractionation Homo sapiens
87 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 RPA3 6119
Proximity Label-MS Homo sapiens
89 RPS13 6207
Co-fractionation Homo sapiens
90 RPL23A 6147
Co-fractionation Homo sapiens
91 FKBP3 2287
Co-fractionation Homo sapiens
92 DDRGK1 65992
Affinity Capture-MS Homo sapiens
93 Eif3e 16341
Affinity Capture-MS Mus musculus
94 BMP4 652
Affinity Capture-MS Homo sapiens
95 DDX52  
Co-fractionation Homo sapiens
96 C9orf72  
Affinity Capture-MS Homo sapiens
97 ILF3 3609
Affinity Capture-MS Homo sapiens
98 DDX55  
Co-fractionation Homo sapiens
99 RPS16 6217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 HIF1A 3091
Affinity Capture-MS Homo sapiens
101 PA2G4 5036
Co-fractionation Homo sapiens
102 FTL 2512
Affinity Capture-MS Homo sapiens
103 YBX1 4904
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
104 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
105 RPS25 6230
Affinity Capture-MS Homo sapiens
106 HDGF 3068
Affinity Capture-MS Homo sapiens
107 FBXL6  
Affinity Capture-MS Homo sapiens
108 XRCC6 2547
Affinity Capture-MS Homo sapiens
109 ZNF746  
Affinity Capture-MS Homo sapiens
110 HNRNPA2B1 3181
Co-fractionation Homo sapiens
111 SRP19 6728
Affinity Capture-MS Homo sapiens
112 PTBP1 5725
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
113 THRAP3 9967
Affinity Capture-MS Homo sapiens
114 SRP72 6731
Affinity Capture-MS Homo sapiens
115 PTPN2 5771
Affinity Capture-MS Homo sapiens
116 RPL15 6138
Affinity Capture-MS Homo sapiens
117 PRRC2B  
Two-hybrid Homo sapiens
118 ESR1  
Affinity Capture-MS Homo sapiens
119 UFL1 23376
Affinity Capture-MS Homo sapiens
120 RPS20 6224
Affinity Capture-MS Homo sapiens
121 API5 8539
Affinity Capture-MS Homo sapiens
122 SMYD2 56950
Affinity Capture-MS Homo sapiens
123 HLA-DPA1  
Affinity Capture-MS Homo sapiens
124 TRIP4 9325
Affinity Capture-MS Homo sapiens
125 RPL7A 6130
Affinity Capture-MS Homo sapiens
126 CIT 11113
Affinity Capture-MS Homo sapiens
127 RPL8 6132
Affinity Capture-MS Homo sapiens
128 HNRNPF 3185
Affinity Capture-MS Homo sapiens
129 BRD1 23774
Affinity Capture-MS Homo sapiens
130 ORC1  
Affinity Capture-MS Homo sapiens
131 LDLR 3949
Positive Genetic Homo sapiens
132 CUL7 9820
Affinity Capture-MS Homo sapiens
133 KRAS 3845
Negative Genetic Homo sapiens
134 HNRNPU 3192
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
135 GNB2L1 10399
Affinity Capture-MS Homo sapiens
136 RC3H1 149041
Affinity Capture-MS Homo sapiens
137 NEMF 9147
Affinity Capture-MS Homo sapiens
138 REPIN1  
Affinity Capture-MS Homo sapiens
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