Gene description for SMYD2
Gene name SET and MYND domain containing 2
Gene symbol SMYD2
Other names/aliases HSKM-B
KMT3C
ZMYND14
Species Homo sapiens
 Database cross references - SMYD2
ExoCarta ExoCarta_56950
Vesiclepedia VP_56950
Entrez Gene 56950
HGNC 20982
MIM 610663
UniProt Q9NRG4  
 SMYD2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SMYD2
Molecular Function
    RNA polymerase II core binding GO:0000993 ISS
    protein binding GO:0005515 IPI
    histone methyltransferase activity (H3-K36 specific) GO:0046975 ISS
    protein-lysine N-methyltransferase activity GO:0016279 IDA
    p53 binding GO:0002039 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    chromatin organization GO:0006325 TAS
    transcription, DNA-templated GO:0006351 IEA
    histone H3-K36 methylation GO:0010452 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    peptidyl-lysine monomethylation GO:0018026 IDA
    peptidyl-lysine dimethylation GO:0018027 ISS
    negative regulation of cell proliferation GO:0008285 ISS
    regulation of DNA damage response, signal transduction by p53 class mediator GO:0043516 IMP
Subcellular Localization
    cytosol GO:0005829 ISS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 ISS
    nucleus GO:0005634 ISS
 Experiment description of studies that identified SMYD2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMYD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AXIN1  
Affinity Capture-MS Homo sapiens
2 EPB41L3 23136
Affinity Capture-MS Homo sapiens
3 AKAP11 11215
Affinity Capture-MS Homo sapiens
4 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
5 CDC37 11140
Affinity Capture-MS Homo sapiens
6 GSK3B 2932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 CLASP2 23122
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SMYD2 is involved
PathwayEvidenceSource
PKMTs methylate histone lysines TAS Reactome





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