Experiment description of studies that identified AKAP11 in exosomes

1 Experiment ID 226 MISEV standards ✔ EM EV Biophysical techniques ✔ GAPDH|CD9|FLOT1 EV Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 EV Negative markers ✘ EV Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 236 MISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Enriched markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 488 MISEV standards ✔ EM EV Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for AKAP11

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PPP1CC 5501 Affinity Capture-Western Homo sapiens 2 PPP1CA 5499 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 3 PRKAR2A 5576 Protein-peptide Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 4 PRKAR1A 5573 Invivo Homo sapiens 5 PPP1CB 5500 Affinity Capture-Western Homo sapiens 6 SMYD2 56950 Affinity Capture-MS Homo sapiens 7 GSK3B 2932 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 8 PRKAR2B 5577 Invivo Homo sapiens View the network

Pathways in which AKAP11 is involved

No pathways found