Gene description for SFRP4
Gene name secreted frizzled-related protein 4
Gene symbol SFRP4
Other names/aliases FRP-4
FRPHE
sFRP-4
Species Homo sapiens
 Database cross references - SFRP4
ExoCarta ExoCarta_6424
Vesiclepedia VP_6424
Entrez Gene 6424
HGNC 10778
MIM 606570
UniProt Q6FHJ7  
 SFRP4 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for SFRP4
Molecular Function
    protein binding GO:0005515 IPI
    Wnt-protein binding GO:0017147 IBA
    Wnt-protein binding GO:0017147 IDA
    Wnt-protein binding GO:0017147 IPI
    Wnt-protein binding GO:0017147 NAS
Biological Process
    positive regulation of receptor internalization GO:0002092 IDA
    negative regulation of cell population proliferation GO:0008285 IMP
    response to hormone GO:0009725 NAS
    positive regulation of gene expression GO:0010628 IDA
    cell differentiation GO:0030154 IEA
    negative regulation of Wnt signaling pathway GO:0030178 NAS
    regulation of BMP signaling pathway GO:0030510 ISS
    non-canonical Wnt signaling pathway GO:0035567 IBA
    positive regulation of apoptotic process GO:0043065 IMP
    negative regulation of DNA-binding transcription factor activity GO:0043433 IDA
    positive regulation of epidermal cell differentiation GO:0045606 IDA
    phosphate ion homeostasis GO:0055062 IDA
    canonical Wnt signaling pathway GO:0060070 IBA
    bone morphogenesis GO:0060349 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 IDA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    positive regulation of canonical Wnt signaling pathway GO:0090263 IGI
    positive regulation of keratinocyte apoptotic process GO:1902174 IDA
    negative regulation of non-canonical Wnt signaling pathway GO:2000051 ISS
    negative regulation of sodium-dependent phosphate transport GO:2000119 IDA
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cell surface GO:0009986 IDA
 Experiment description of studies that identified SFRP4 in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for SFRP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP2R3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HSD17B11 51170
Two-hybrid Homo sapiens
3 RASSF8 11228
Affinity Capture-MS Homo sapiens
4 FBN2 2201
Affinity Capture-MS Homo sapiens
5 AKAP11 11215
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GAPDHS 26330
Affinity Capture-MS Homo sapiens
7 CALR 811
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 FBLN1 2192
Affinity Capture-MS Homo sapiens
9 ANKRD17 26057
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 DCAF16 54876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 KBTBD7  
Affinity Capture-MS Homo sapiens
12 TUBB1 81027
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 DCAF8 50717
Affinity Capture-MS Homo sapiens
14 ANKHD1 54882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 UBE2O 63893
Affinity Capture-MS Homo sapiens
16 B3GALNT2 148789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 DICER1  
Affinity Capture-MS Homo sapiens
18 SIRT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 PASK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 PRKAR1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 LRP1B 53353
Affinity Capture-MS Homo sapiens
22 DCAF5  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SFRP4 is involved
No pathways found





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