Gene description for GAPDHS
Gene name glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Gene symbol GAPDHS
Other names/aliases GAPD2
GAPDH-2
GAPDS
HSD-35
Species Homo sapiens
 Database cross references - GAPDHS
ExoCarta ExoCarta_26330
Vesiclepedia VP_26330
Entrez Gene 26330
HGNC 24864
MIM 609169
UniProt O14556  
 GAPDHS identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for GAPDHS
Molecular Function
    glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 IBA
    glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 TAS
    protein binding GO:0005515 IPI
    NADP binding GO:0050661 IEA
    NAD binding GO:0051287 IEA
Biological Process
    glucose metabolic process GO:0006006 IEA
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 IEA
    flagellated sperm motility GO:0030317 ISS
    positive regulation of glycolytic process GO:0045821 TAS
Subcellular Localization
    nucleus GO:0005634 HDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified GAPDHS in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
4
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GAPDHS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZMYND19  
Two-hybrid Homo sapiens
2 ISX  
Affinity Capture-MS Homo sapiens
3 MAFF 23764
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LINGO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CCDC127  
Affinity Capture-MS Homo sapiens
6 ADCK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ELAVL2 1993
Affinity Capture-MS Homo sapiens
8 SLC45A2 51151
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SH3BP4 23677
Two-hybrid Homo sapiens
10 LPAR6  
Affinity Capture-MS Homo sapiens
11 KLC3  
Affinity Capture-MS Homo sapiens
12 LOC146880  
Affinity Capture-MS Homo sapiens
13 MFSD5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ATM 472
Affinity Capture-MS Homo sapiens
15 CLEC12A  
Affinity Capture-MS Homo sapiens
16 UNKL  
Affinity Capture-MS Homo sapiens
17 DNAJC15  
Affinity Capture-MS Homo sapiens
18 SOX2  
Affinity Capture-MS Homo sapiens
19 APP 351
Reconstituted Complex Homo sapiens
20 PCBP3 54039
Affinity Capture-MS Homo sapiens
21 LRRC37A2  
Affinity Capture-MS Homo sapiens
22 ZNF623  
Affinity Capture-MS Homo sapiens
23 DPPA4  
Affinity Capture-MS Homo sapiens
24 CEACAM21  
Affinity Capture-MS Homo sapiens
25 INSL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ZNF74  
Affinity Capture-MS Homo sapiens
27 ELOVL2  
Affinity Capture-MS Homo sapiens
28 TCTEX1D1  
Affinity Capture-MS Homo sapiens
29 CKMT1A 548596
Affinity Capture-MS Homo sapiens
30 SLC39A11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PRC1 9055
Affinity Capture-MS Homo sapiens
32 ZNF664  
Affinity Capture-MS Homo sapiens
33 LETM2  
Affinity Capture-MS Homo sapiens
34 CTH 1491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 NUSAP1 51203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ZNF576  
Affinity Capture-MS Homo sapiens
37 GPX8 493869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 BHMT2 23743
Affinity Capture-MS Homo sapiens
39 ZNF780A  
Affinity Capture-MS Homo sapiens
40 CCDC102A 92922
Affinity Capture-MS Homo sapiens
41 C12orf4  
Affinity Capture-MS Homo sapiens
42 CHMP4C 92421
Affinity Capture-MS Homo sapiens
43 TMEM220  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GRIA1  
Affinity Capture-MS Homo sapiens
45 SPG21 51324
Affinity Capture-MS Homo sapiens
46 C19orf44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ATP1B4  
Affinity Capture-MS Homo sapiens
48 RNFT1  
Affinity Capture-MS Homo sapiens
49 GALNT16 57452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 C12orf50  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TRA2A 29896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 PURG  
Affinity Capture-MS Homo sapiens
53 ASCL1  
Affinity Capture-MS Homo sapiens
54 DDX20 11218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 NOL7  
Affinity Capture-MS Homo sapiens
56 PRDM16 63976
Affinity Capture-MS Homo sapiens
57 NCAPD3 23310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 OR12D3  
Affinity Capture-MS Homo sapiens
59 SRBD1  
Affinity Capture-MS Homo sapiens
60 OR4E2  
Affinity Capture-MS Homo sapiens
61 CKAP5 9793
Affinity Capture-MS Homo sapiens
62 CFAP46  
Affinity Capture-MS Homo sapiens
63 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
64 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
65 IL15  
Affinity Capture-MS Homo sapiens
66 ITK 3702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 ANXA1 301
Protein-peptide Homo sapiens
68 DNAJB5  
Affinity Capture-MS Homo sapiens
69 CSPG5 10675
Affinity Capture-MS Homo sapiens
70 FOXS1  
Affinity Capture-MS Homo sapiens
71 DSN1 79980
Affinity Capture-MS Homo sapiens
72 PSG11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 CCDC59  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 APOF 319
Affinity Capture-MS Homo sapiens
75 RNF40 9810
Affinity Capture-MS Homo sapiens
76 HYAL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 C16orf70  
Affinity Capture-MS Homo sapiens
78 LOC613266  
Affinity Capture-MS Homo sapiens
79 RASGEF1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 SECTM1 6398
Affinity Capture-MS Homo sapiens
81 SFRP4 6424
Affinity Capture-MS Homo sapiens
82 ICA1  
Affinity Capture-MS Homo sapiens
83 PCDHGC3 5098
Affinity Capture-MS Homo sapiens
84 SRSF5 6430
Affinity Capture-MS Homo sapiens
85 SLC5A2 6524
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 ARHGEF16 27237
Affinity Capture-MS Homo sapiens
87 CMTM2  
Affinity Capture-MS Homo sapiens
88 CBR1 873
Affinity Capture-MS Homo sapiens
89 THAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 NTHL1  
Affinity Capture-MS Homo sapiens
91 CCDC50 152137
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 AURKB 9212
Affinity Capture-MS Homo sapiens
93 S100A7L2  
Affinity Capture-MS Homo sapiens
94 ZSCAN5A  
Affinity Capture-MS Homo sapiens
95 SPZ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 SLC25A18  
Affinity Capture-MS Homo sapiens
97 COA5  
Affinity Capture-MS Homo sapiens
98 PROK2  
Affinity Capture-MS Homo sapiens
99 AP5B1 91056
Affinity Capture-MS Homo sapiens
100 NPIPB6  
Affinity Capture-MS Homo sapiens
101 KLRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 FBXO15 201456
Affinity Capture-MS Homo sapiens
103 CTSF 8722
Affinity Capture-MS Homo sapiens
104 SUMO1P1  
Affinity Capture-MS Homo sapiens
105 DYM  
Affinity Capture-MS Homo sapiens
106 BATF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
108 TRIP6 7205
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 MKRN1 23608
Affinity Capture-MS Homo sapiens
110 MEIOB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 SWSAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 UBA5 79876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 CDA 978
Affinity Capture-MS Homo sapiens
114 PCDHGA7 56108
Affinity Capture-MS Homo sapiens
115 ABHD10 55347
Affinity Capture-MS Homo sapiens
116 PTPN20B  
Affinity Capture-MS Homo sapiens
117 ODF2L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 LMAN1 3998
Affinity Capture-MS Homo sapiens
119 AVPI1  
Affinity Capture-MS Homo sapiens
120 C17orf89  
Affinity Capture-MS Homo sapiens
121 CDC6  
Affinity Capture-MS Homo sapiens
122 TMEM161B  
Affinity Capture-MS Homo sapiens
123 RAB32 10981
Affinity Capture-MS Homo sapiens
124 ZDHHC23  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 TMLHE 55217
Affinity Capture-MS Homo sapiens
126 TUT1  
Affinity Capture-MS Homo sapiens
127 ANKH 56172
Affinity Capture-MS Homo sapiens
128 ZNF684  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 PDYN  
Affinity Capture-MS Homo sapiens
130 GDE1 51573
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 RABGEF1 27342
Affinity Capture-MS Homo sapiens
132 MRPS18B 28973
Affinity Capture-MS Homo sapiens
133 GAPDH 2597
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 PHACTR4 65979
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 KCNA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 FAM117B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 FECH 2235
Affinity Capture-MS Homo sapiens
138 TMEM38B  
Affinity Capture-MS Homo sapiens
139 TRPT1 83707
Affinity Capture-MS Homo sapiens
140 SAMD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 SLC25A52  
Affinity Capture-MS Homo sapiens
142 ULK3 25989
Affinity Capture-MS Homo sapiens
143 TAP1 6890
Affinity Capture-MS Homo sapiens
144 GLRB  
Affinity Capture-MS Homo sapiens
145 SNX29  
Affinity Capture-MS Homo sapiens
146 DCP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
147 LRRTM3  
Affinity Capture-MS Homo sapiens
148 MGST3 4259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 CUL4A 8451
Affinity Capture-MS Homo sapiens
150 DARS2 55157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 L3MBTL4  
Affinity Capture-MS Homo sapiens
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