Gene description for BHMT2
Gene name betaine--homocysteine S-methyltransferase 2
Gene symbol BHMT2
Other names/aliases -
Species Homo sapiens
 Database cross references - BHMT2
ExoCarta ExoCarta_23743
Vesiclepedia VP_23743
Entrez Gene 23743
HGNC 1048
MIM 605932
UniProt Q9H2M3  
 BHMT2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Urine 19056867    
 Gene ontology annotations for BHMT2
Molecular Function
    zinc ion binding GO:0008270 IDA
    S-adenosylmethionine-homocysteine S-methyltransferase activity GO:0008898 IEA
    betaine-homocysteine S-methyltransferase activity GO:0047150 IDA
    S-methylmethionine-homocysteine S-methyltransferase activity GO:0061627 IBA
    S-methylmethionine-homocysteine S-methyltransferase activity GO:0061627 IDA
Biological Process
    amino-acid betaine metabolic process GO:0006577 IDA
    methylation GO:0032259 IEA
    S-methylmethionine metabolic process GO:0033477 IDA
    S-adenosylmethionine metabolic process GO:0046500 IDA
    L-methionine salvage GO:0071267 IBA
    L-methionine salvage GO:0071267 IDA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified BHMT2 in sEVs
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for BHMT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 TBCE 6905
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 GAPDHS 26330
Affinity Capture-MS Homo sapiens
4 BIRC7  
Affinity Capture-MS Homo sapiens
5 BHMT 635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CUL4A 8451
Affinity Capture-MS Homo sapiens
7 INPPL1 3636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which BHMT2 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome
Sulfur amino acid metabolism TAS Reactome





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