Gene description for GALNT16
Gene name polypeptide N-acetylgalactosaminyltransferase 16
Gene symbol GALNT16
Other names/aliases GALNACT16
GALNTL1
GalNAc-T16
Species Homo sapiens
 Database cross references - GALNT16
ExoCarta ExoCarta_57452
Vesiclepedia VP_57452
Entrez Gene 57452
HGNC 23233
MIM 615132
UniProt Q8N428  
 GALNT16 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GALNT16
Molecular Function
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IBA
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IDA
    carbohydrate binding GO:0030246 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    protein O-linked glycosylation GO:0006493 IBA
    protein O-linked glycosylation via serine GO:0018242 IDA
    protein O-linked glycosylation via threonine GO:0018243 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi apparatus GO:0005794 IBA
 Experiment description of studies that identified GALNT16 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GALNT16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 REEP5 7905
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ARHGAP17 55114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SCN4A  
Affinity Capture-MS Homo sapiens
4 CLEC12B  
Affinity Capture-MS Homo sapiens
5 NIPAL1  
Affinity Capture-MS Homo sapiens
6 GAPDHS 26330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ST14 6768
Affinity Capture-MS Homo sapiens
8 PRPSAP2 5636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PARM1  
Affinity Capture-MS Homo sapiens
10 CCL3  
Affinity Capture-MS Homo sapiens
11 CSPG5 10675
Affinity Capture-MS Homo sapiens
12 IPO8 10526
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 RMND1 55005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TMPRSS13  
Affinity Capture-MS Homo sapiens
15 SAAL1 113174
Affinity Capture-MS Homo sapiens
16 SLC39A11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SCPEP1 59342
Affinity Capture-MS Homo sapiens
18 PEX19 5824
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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