Gene description for CSPG5
Gene name chondroitin sulfate proteoglycan 5 (neuroglycan C)
Gene symbol CSPG5
Other names/aliases NGC
Species Homo sapiens
 Database cross references - CSPG5
ExoCarta ExoCarta_10675
Vesiclepedia VP_10675
Entrez Gene 10675
HGNC 2467
MIM 606775
UniProt O95196  
 CSPG5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CSPG5
Molecular Function
    protein binding GO:0005515 IPI
    growth factor activity GO:0008083 TAS
Biological Process
    cytoskeleton organization GO:0007010 ISS
    signal transduction GO:0007165 IEA
    nervous system development GO:0007399 TAS
    axon regeneration GO:0031103 IEA
    intracellular transport GO:0046907 TAS
    cell projection morphogenesis GO:0048858 IBA
    glial cell projection elongation GO:0106091 ISS
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISS
    regulation of synaptic vesicle exocytosis GO:2000300 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    extracellular region GO:0005576 TAS
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IDA
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IEA
    membrane GO:0016020 TAS
    Golgi-associated vesicle membrane GO:0030660 IDA
    lysosomal lumen GO:0043202 TAS
    synapse GO:0045202 IBA
    postsynaptic membrane GO:0045211 IEA
    glutamatergic synapse GO:0098978 IEA
    GABA-ergic synapse GO:0098982 IEA
 Experiment description of studies that identified CSPG5 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CSPG5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C1GALT1 56913
Affinity Capture-MS Homo sapiens
2 SACM1L 22908
Affinity Capture-MS Homo sapiens
3 COLEC12 81035
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TARBP1 6894
Affinity Capture-MS Homo sapiens
5 CDS1  
Affinity Capture-MS Homo sapiens
6 SCAMP1 9522
Affinity Capture-MS Homo sapiens
7 C1GALT1C1 29071
Affinity Capture-MS Homo sapiens
8 GAPDHS 26330
Affinity Capture-MS Homo sapiens
9 HS2ST1 9653
Affinity Capture-MS Homo sapiens
10 GALNT16 57452
Affinity Capture-MS Homo sapiens
11 LMBR1  
Affinity Capture-MS Homo sapiens
12 TMX1 81542
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ERBB3 2065
Reconstituted Complex Homo sapiens
15 PTPN2 5771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 UFL1 23376
Affinity Capture-MS Homo sapiens
17 SCN4A  
Affinity Capture-MS Homo sapiens
18 CAPZB 832
Affinity Capture-MS Homo sapiens
19 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
20 DIAPH1 1729
Affinity Capture-MS Homo sapiens
21 DPP9 91039
Affinity Capture-MS Homo sapiens
22 TEFM  
Affinity Capture-MS Homo sapiens
23 FAM115C  
Affinity Capture-MS Homo sapiens
24 MLX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 GALNT7 51809
Affinity Capture-MS Homo sapiens
26 HIRA  
Affinity Capture-MS Homo sapiens
27 GALNT2 2590
Affinity Capture-MS Homo sapiens
28 NOG 9241
Affinity Capture-MS Homo sapiens
29 ALG11  
Affinity Capture-MS Homo sapiens
30 SRP14 6727
Affinity Capture-MS Homo sapiens
31 GOPC 57120
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here