Gene description for HS2ST1
Gene name heparan sulfate 2-O-sulfotransferase 1
Gene symbol HS2ST1
Other names/aliases dJ604K5.2
Species Homo sapiens
 Database cross references - HS2ST1
ExoCarta ExoCarta_9653
Vesiclepedia VP_9653
Entrez Gene 9653
HGNC 5193
MIM 604844
UniProt Q7LGA3  
 HS2ST1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for HS2ST1
Molecular Function
    heparan sulfate 2-O-sulfotransferase activity GO:0004394 IBA
    heparan sulfate 2-O-sulfotransferase activity GO:0004394 ISS
    heparan sulfate 2-O-sulfotransferase activity GO:0004394 TAS
    protein binding GO:0005515 IPI
Biological Process
    gene expression GO:0010467 IEA
    heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process GO:0015014 IBA
    heparan sulfate proteoglycan biosynthetic process, enzymatic modification GO:0015015 IBA
    heparan sulfate proteoglycan biosynthetic process, enzymatic modification GO:0015015 TAS
    heparin metabolic process GO:0030202 IEA
    ureteric bud formation GO:0060676 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified HS2ST1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HS2ST1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DPY19L1 23333
Affinity Capture-MS Homo sapiens
2 TGOLN2 10618
Affinity Capture-MS Homo sapiens
3 KIAA1147  
Affinity Capture-MS Homo sapiens
4 RAMP3  
Affinity Capture-MS Homo sapiens
5 VPS35 55737
Affinity Capture-MS Homo sapiens
6 AGPAT5 55326
Affinity Capture-MS Homo sapiens
7 DNAJC11 55735
Affinity Capture-MS Homo sapiens
8 ADRB2  
Two-hybrid Homo sapiens
9 TMEM184A  
Affinity Capture-MS Homo sapiens
10 ATP12A 479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 C9orf78 51759
Affinity Capture-MS Homo sapiens
12 APP 351
Affinity Capture-MS Homo sapiens
13 PDE2A  
Affinity Capture-MS Homo sapiens
14 SERINC2 347735
Affinity Capture-MS Homo sapiens
15 CAPZB 832
Affinity Capture-MS Homo sapiens
16 PTDSS2 81490
Affinity Capture-MS Homo sapiens
17 UXS1 80146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ITGA7 3679
Affinity Capture-MS Homo sapiens
19 DIAPH3 81624
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 TBC1D23 55773
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 GPRC5B 51704
Affinity Capture-MS Homo sapiens
22 MARCH5  
Affinity Capture-MS Homo sapiens
23 SOGA3  
Affinity Capture-MS Homo sapiens
24 ATP2A3 489
Affinity Capture-MS Homo sapiens
25 PGRMC2 10424
Affinity Capture-MS Homo sapiens
26 NLGN2 57555
Affinity Capture-MS Homo sapiens
27 ERMP1 79956
Affinity Capture-MS Homo sapiens
28 SLC7A11 23657
Affinity Capture-MS Homo sapiens
29 CD3E 916
Affinity Capture-MS Homo sapiens
30 HLA-G 3135
Affinity Capture-MS Homo sapiens
31 ATP1A3 478
Affinity Capture-MS Homo sapiens
32 PAM 5066
Affinity Capture-MS Homo sapiens
33 NOMO1 23420
Affinity Capture-MS Homo sapiens
34 FOXA1  
Affinity Capture-MS Homo sapiens
35 PARK2  
Affinity Capture-MS Homo sapiens
36 AMFR 267
Affinity Capture-MS Homo sapiens
37 NMNAT3 349565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 RNF213 57674
Affinity Capture-MS Homo sapiens
39 SDF2L1 23753
Affinity Capture-MS Homo sapiens
40 OGT 8473
Reconstituted Complex Homo sapiens
41 PIGT 51604
Affinity Capture-MS Homo sapiens
42 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
43 DNAJA1 3301
Affinity Capture-MS Homo sapiens
44 LRBA 987
Affinity Capture-MS Homo sapiens
45 PIGS 94005
Affinity Capture-MS Homo sapiens
46 HECTD1 25831
Affinity Capture-MS Homo sapiens
47 STX5 6811
Affinity Capture-MS Homo sapiens
48 CSPG5 10675
Affinity Capture-MS Homo sapiens
49 PNMA6A  
Affinity Capture-MS Homo sapiens
50 SLC7A1 6541
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 P2RX4 5025
Affinity Capture-MS Homo sapiens
52 CDS2 8760
Affinity Capture-MS Homo sapiens
53 GPAA1 8733
Affinity Capture-MS Homo sapiens
54 TMX3 54495
Affinity Capture-MS Homo sapiens
55 CANX 821
Affinity Capture-MS Homo sapiens
56 PON2 5445
Affinity Capture-MS Homo sapiens
57 ASIC2  
Affinity Capture-MS Homo sapiens
58 GPR182  
Affinity Capture-MS Homo sapiens
59 ERGIC3 51614
Affinity Capture-MS Homo sapiens
60 P2RY2 5029
Affinity Capture-MS Homo sapiens
61 CUL3 8452
Affinity Capture-MS Homo sapiens
62 ACAD10  
Affinity Capture-MS Homo sapiens
63 TMEM258  
Affinity Capture-MS Homo sapiens
64 YIPF5 81555
Affinity Capture-MS Homo sapiens
65 CYFIP2 26999
Affinity Capture-MS Homo sapiens
66 SAAL1 113174
Affinity Capture-MS Homo sapiens
67 DCAF6 55827
Affinity Capture-MS Homo sapiens
68 CHRNA9  
Affinity Capture-MS Homo sapiens
69 LMF1 64788
Affinity Capture-MS Homo sapiens
70 RPA3 6119
Proximity Label-MS Homo sapiens
71 ATP2A1 487
Affinity Capture-MS Homo sapiens
72 DDRGK1 65992
Affinity Capture-MS Homo sapiens
73 DYM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 HEATR1 55127
Affinity Capture-MS Homo sapiens
75 HLA-A 3105
Affinity Capture-MS Homo sapiens
76 FUT1  
Affinity Capture-MS Homo sapiens
77 PON3 5446
Affinity Capture-MS Homo sapiens
78 TMED10 10972
Affinity Capture-MS Homo sapiens
79 USE1 55850
Affinity Capture-MS Homo sapiens
80 SPG7  
Affinity Capture-MS Homo sapiens
81 ABCB8 11194
Affinity Capture-MS Homo sapiens
82 SCFD2 152579
Affinity Capture-MS Homo sapiens
83 PEX19 5824
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 TNFSF14  
Affinity Capture-MS Homo sapiens
85 EPHX4 253152
Affinity Capture-MS Homo sapiens
86 TSPAN17 26262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 PIGK 10026
Affinity Capture-MS Homo sapiens
88 PIGU 128869
Affinity Capture-MS Homo sapiens
89 VAC14 55697
Affinity Capture-MS Homo sapiens
90 ERGIC1 57222
Affinity Capture-MS Homo sapiens
91 MGST1 4257
Affinity Capture-MS Homo sapiens
92 SEC62 7095
Affinity Capture-MS Homo sapiens
93 SPPL2B 56928
Affinity Capture-MS Homo sapiens
94 P2RX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 CKAP4 10970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 USP9Y 8287
Affinity Capture-MS Homo sapiens
97 BRI3BP 140707
Affinity Capture-MS Homo sapiens
98 FPR2  
Affinity Capture-MS Homo sapiens
99 SLC5A3 6526
Affinity Capture-MS Homo sapiens
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